Abstract
Abstract Background Gloriosa superba L. belongs to Colchicaceae, which is an important medicinal plant containing high-priced alkaloid colchicines and other potent phytochemicals. Due to its extensive importance at the industrial level, this plant is overexploited. Moreover, indiscriminate harvesting for raw material leads to a decline in the population of this plant in the natural environment. Thus, the present study deals with the optimization of colchicine accumulation from the different intervals of in vitro and in vivo tubers of Gloriosa . Result To obtain in vitro tuberization, shoot tip explants were inoculated on Murashige and Skoog medium prepared with 3 mg/l BA and 0.5 mg/l Kn + 1 mg/l 2, 4-D followed by 2 mg/l BA + 0.2 mg/l NAA. In the high-performance thin-layer chromatography study (HPTLC), the linearity range of colchicine was set at a concentration range of 100–1000 ng/spot with a regression value (r) of 0.99. Its R f value (0.25) was recorded at 254 nm. The colchicine amount in the in vivo tuber was 7.75 ± 0.25% dry weight, while the nearby amount of 7.7 ± 0.40% dry weight of colchicine was produced from 2 weeks in vitro old tuber. This value was followed by the 4th-week old tuber with 6.35 ± 0.17% dry weight and then a gradual decrease in its accumulation. Conclusion The significant results for the accumulation of colchicine at different stages were observed. Hence, this strategy of colchicine production creates a new possibility for improved production of colchicine under in vitro conditions which will be helpful to various pharmaceutical industries without damaging the plants from the natural environment. Graphical abstract
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Claims 5
Claim · model-written summary
The in vivo tuber of Gloriosa superba contained colchicine at 7.75 ± 0.25% dry weight.
- Taxon
- Gloriosa superba L.
Gloriosa superbae0 - Organ / stage
- in vivo tubere1
- Outcome
- colchicine amounte1
- Value
- 7.75 ± 0.25% dry weighte1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gloriosa superba L. belongs to Colchicaceae”
e1“The colchicine amount in the in vivo tuber was 7.75 ± 0.25% dry weight”
Claim · model-written summary
Colchicine produced from the 2-week-old in vitro tuber was 7.7 ± 0.40% dry weight, close to the in vivo tuber amount.
- Taxon
- Gloriosa superba L.Gloriosa superbae0
- Organ / stage
- 2 weeks in vitro old tubere1
- Outcome
- colchicinee1
- Value
- 7.7 ± 0.40% dry weighte1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gloriosa superba L. belongs to Colchicaceae”
e1“the nearby amount of 7.7 ± 0.40% dry weight of colchicine was produced from 2 weeks in vitro old tuber”
Claim · model-written summary
The 4th-week old in vitro tuber had colchicine accumulation of 6.35 ± 0.17% dry weight, followed by a gradual decrease in accumulation.
- Taxon
- Gloriosa superba L.Gloriosa superbae0
- Organ / stage
- 4th-week old tubere1
- Outcome
- accumulatione1
- Direction
- gradual decreasee1
- Value
- 6.35 ± 0.17% dry weighte1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gloriosa superba L. belongs to Colchicaceae”
e1“followed by the 4th-week old tuber with 6.35 ± 0.17% dry weight and then a gradual decrease in its accumulation”
Claim · model-written summary
The HPTLC method for colchicine was linear over 100-1000 ng/spot with a regression value (r) of 0.99.
Verbatim
e0“the linearity range of colchicine was set at a concentration range of 100–1000 ng/spot with a regression value (r) of 0.99”
Claim · model-written summary
Colchicine showed an R f value of 0.25 recorded at 254 nm in HPTLC.
Verbatim
e0“Its R f value (0.25) was recorded at 254 nm”