Abstract
Abstract Drought is one of the main abiotic stresses adversely affecting maize growth and grain yield. Identifying drought tolerance‐related genes and breeding varieties with enhanced tolerance are effective strategies for minimizing the effects of drought stress. In this study, the leaf relative water content (LRWC) was used for evaluating drought tolerance. QTL‐seq analysis of 419 F 2 individuals from a cross between ZhengT22 (the drought‐tolerant line with high LRWC) and ZhengA88 (the drought‐sensitive line with low LRWC) revealed four LRWC‐related QTLs ( qLRWC2 , qLRWC10a , qLRWC10b , and qLRWC10c ) in maize seedlings under water deficit. Of these QTLs, qLRWC2 was located in a 2.03‐Mb interval on chromosome 2, whereas qLRWC10a , qLRWC10b , and qLRWC10c were located in 2.85‐, 3.99‐, and 2.05‐Mb intervals, respectively, on chromosome 10, and the 93 genes contained the variation loci locating in the four QTLs regions. To identify the candidate genes within the QTLs, an RNA‐seq analysis was performed for the parents exposed to water deficit. Seven genes with effective variation loci showed significant difference in expression either in ZhengA88 or ZhengT22 in response to water deficit. Moreover, among the genes, ZmPrx64 , ZmCIPK , HSP90 , and ABCG34 have all been shown to be related to water stress in the previous studies. Thus, they are primary considered as the potential candidate genes controlling LRWC under water deficit at the seeding stage of maize in this study. These findings will help clarify the molecular basis of drought tolerance in maize seedlings and may be relevant for future functional analysis and for breeding drought‐tolerant maize varieties.
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Claims 9
Claim · model-written summary
ZhengT22 had high LRWC and ZhengA88 low LRWC.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“ZhengT22 (the drought‐tolerant line with high LRWC) and ZhengA88 (the drought‐sensitive line with low LRWC)”
Claim · model-written summary
Four QTLs were identified for maize seedling LRWC under water deficit.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“revealed four LRWC‐related QTLs ( qLRWC2 , qLRWC10a , qLRWC10b , and qLRWC10c ) in maize seedlings under water deficit”
Claim · model-written summary
qLRWC2 mapped to a 2.03-Mb interval on chromosome 2.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“qLRWC2 was located in a 2.03‐Mb interval on chromosome 2”
Claim · model-written summary
qLRWC10a mapped to a 2.85-Mb interval on chromosome 10.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“qLRWC10a , qLRWC10b , and qLRWC10c were located in 2.85‐, 3.99‐, and 2.05‐Mb intervals, respectively, on chromosome 10”
Claim · model-written summary
qLRWC10b mapped to a 3.99-Mb interval on chromosome 10.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“qLRWC10a , qLRWC10b , and qLRWC10c were located in 2.85‐, 3.99‐, and 2.05‐Mb intervals, respectively, on chromosome 10”
Claim · model-written summary
qLRWC10c mapped to a 2.05-Mb interval on chromosome 10.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“qLRWC10a , qLRWC10b , and qLRWC10c were located in 2.85‐, 3.99‐, and 2.05‐Mb intervals, respectively, on chromosome 10”
Claim · model-written summary
Ninety-three genes contained variation loci in the four QTL regions.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“the 93 genes contained the variation loci locating in the four QTLs regions”
Claim · model-written summary
Seven variant-bearing genes had significantly different expression in one or both parents after water deficit.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“Seven genes with effective variation loci showed significant difference in expression either in ZhengA88 or ZhengT22 in response to water deficit”
Claim · model-written summary
ZmPrx64, ZmCIPK, HSP90 and ABCG34 were proposed as candidate genes for LRWC control.
- Organ / stage
- seeding stagee0
- Gene
- ZmPrx64 , ZmCIPK , HSP90 , and ABCG34e0
- Outcome
- potential candidate genes controlling LRWC under water deficite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“ZmPrx64 , ZmCIPK , HSP90 , and ABCG34 have all been shown to be related to water stress in the previous studies. Thus, they are primary considered as the potential candidate genes controlling LRWC under water deficit at the seeding stage of maize”