Abstract
ABSTRACT A modified cry 9Aa2 gene under the transcriptional control of the CaMV 35S promoter was transferred to four potato cultivars (‘Iwa’, ‘Karaka’, ‘Pacific’ and ‘Red Rascal’) using Agrobacterium ‐mediated gene transfer. Field plots were established for 170 independently regenerated transgenic lines derived from 113 transformation events. Phenotypic changes such as stunted plants, reduced vigour and/or leaf puckering, were observed in 29% of these lines. Foliage from the remaining 117 lines inhibited larval growth of potato tuber moth ( Phthorimaea operculella Zeller) compared with non‐transgenic controls. A high correlation was observed between greenhouse‐ and field‐grown foliage for insect resistance using a detached leaf bioassay. Independently regenerated lines recovered from the same transformation event exhibited similar insect resistance. However, these lines derived from the same transformed cell differed markedly in phenotypic appearance and tuber yield. The first shoot regenerated from each transformation event was equivalent to the non‐transgenic control for yield traits in only eight transformation events. A further 19 transformation events exhibited agronomic performance equivalent to the non‐transgenic control when the second or subsequent regenerated shoots were evaluated. In clonal crops, multiple shoots from each transformation event should be independently assessed to ensure recovery of high‐performing transgenic lines.
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Claims 8
Claim · model-written summary
Phenotypic changes occurred in 29% of the potato transgenic lines.
- Taxon
- potato
Solanum tuberosumt0 - Outcome
- Phenotypic changese0
- Value
- 29%e0
- Qualifier
- stunted plants, reduced vigour and/or leaf puckeringe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phenotypic changes such as stunted plants, reduced vigour and/or leaf puckering, were observed in 29% of these lines.”
t0“potato plants”
Claim · model-written summary
Foliage from 117 potato lines inhibited potato tuber moth larval growth compared with non-transgenic controls.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- remaining 117 linese0
- Interactor
- Phthorimaea operculella Zeller
Phthorimaea operculellae0pest - Outcome
- larval growth of potato tuber mothe0
- Direction
- inhibitede0
- Value
- 117 linese0
- Comparator
- non‐transgenic controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Foliage from the remaining 117 lines inhibited larval growth of potato tuber moth ( Phthorimaea operculella Zeller) compared with non‐transgenic controls.”
t0“potato plants”
Claim · model-written summary
Greenhouse and field foliage bioassay resistance results were highly correlated.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- insect resistancee0
- Setting
- greenhouse‐ and field‐grown foliagee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A high correlation was observed between greenhouse‐ and field‐grown foliage for insect resistance using a detached leaf bioassay.”
t0“potato plants”
Claim · model-written summary
Lines regenerated from the same potato transformation event showed similar insect resistance.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- similar insect resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Independently regenerated lines recovered from the same transformation event exhibited similar insect resistance.”
t0“potato plants”
Claim · model-written summary
Lines from the same transformed cell differed markedly in phenotypic appearance and tuber yield.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- phenotypic appearance and tuber yielde0
- Direction
- differed markedlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these lines derived from the same transformed cell differed markedly in phenotypic appearance and tuber yield.”
t0“potato plants”
Claim · model-written summary
The first regenerated shoot matched non-transgenic yield traits in only eight events.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- yield traitse0
- Direction
- equivalente0
- Value
- eight transformation eventse0
- Comparator
- non‐transgenic controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The first shoot regenerated from each transformation event was equivalent to the non‐transgenic control for yield traits in only eight transformation events.”
t0“potato plants”
Claim · model-written summary
Second or later shoots matched control agronomic performance in 19 further events.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- agronomic performancee0
- Direction
- equivalente0
- Value
- 19 transformation eventse0
- Comparator
- non‐transgenic controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A further 19 transformation events exhibited agronomic performance equivalent to the non‐transgenic control when the second or subsequent regenerated shoots were evaluated.”
t0“potato plants”
Claim · model-written summary
Multiple shoots per transformation event should be assessed independently in clonal crops.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- multiple shoots from each transformation event should be independently assessede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In clonal crops, multiple shoots from each transformation event should be independently assessed to ensure recovery of high‐performing transgenic lines.”
t0“potato plants”