Abstract
Garcinia indica popularly known as ‘Kokum’ or Murugalu”, is a medium-sized evergreen tree found in the western-ghats of India. This tree species is highly exploited to produce anti-obesity drugs and culinary purposes. Its population is threatened by overexploitation and loss of habitat. The development of microsatellite markers would help in understanding the genetic structure and further to develop appropriate conservation strategies. In this study, using next generation sequencing platform Illumina Hiseq 2000, we have sequenced the partial genome of G. indica and identified 3725 microsatellites. Forty-eight microsatellite markers were analyzed using 30 accessions. Polymorphism information content (PIC) values ranged from 0.718 to 0.968 with a mean value of 0.922. Allele per locus ranged from 3 to 33 per locus. The probability of identity values ranged from 0.00329 to 0.30489. Cross-species amplification SSR primers in the related species showed a moderate transferability from 12.5 % (for G. morella) to 18.7%(for G. gummigutta)
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Claims 6
Claim · model-written summary
microsatellites were reported for Garcinia indica markers.
- Taxon
- Garcinia indica
Garcinia indicat0 - Outcome
- microsatellitese0
- Value
- 3725e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Garcinia indica”
e0“identified 3725 microsatellites”
Claim · model-written summary
Polymorphism information content (PIC) values were reported for Garcinia indica markers.
- Taxon
- Garcinia indicaGarcinia indicat0
- Outcome
- Polymorphism information content (PIC) valuese0
- Value
- 0.718 to 0.968e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Garcinia indica”
e0“Polymorphism information content (PIC) values ranged from 0.718 to 0.968 with a mean value of 0.922”
Claim · model-written summary
Allele per locus were reported for Garcinia indica markers.
- Taxon
- Garcinia indicaGarcinia indicat0
- Outcome
- Allele per locuse0
- Value
- 3 to 33 per locuse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Garcinia indica”
e0“Allele per locus ranged from 3 to 33 per locus”
Claim · model-written summary
probability of identity values were reported for Garcinia indica markers.
- Taxon
- Garcinia indicaGarcinia indicat0
- Outcome
- probability of identity valuese0
- Value
- 0.00329 to 0.30489e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Garcinia indica”
e0“The probability of identity values ranged from 0.00329 to 0.30489”
Claim · model-written summary
SSR primers showed 12.5 % transferability in G. morella.
- Taxon
- G. morella
Garcinia morellae0 - Outcome
- transferabilitye0
- Value
- 12.5 %e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Garcinia indica”
e0“Cross-species amplification SSR primers in the related species showed a moderate transferability from 12.5 % (for G. morella) to 18.7%(for G. gummigutta)”
Claim · model-written summary
SSR primers showed 18.7% transferability in G. gummigutta.
Not reportedDirection
Verbatim
t0“Garcinia indica”
e0“Cross-species amplification SSR primers in the related species showed a moderate transferability from 12.5 % (for G. morella) to 18.7%(for G. gummigutta)”