Abstract
Begomoviruses are the main biotic threat of tomatoes, resulting in substantial losses worldwide.The coat protein is the most conserved protein in begomoviruses and is essentially required by monopartite begomoviruses for infection in susceptible plants.Its expression in transgenic plants may interfere with the uncoating of the viral DNA upon infection.A study was conducted to investigate the effect of expressing viral coat protein in transgenic plants on the induction of resistance to Tomato yellow leaf curl virus Oman (TYLCV-OM).The synthetic codon-optimized coat protein (CP syn ) of TYLCV-OM was transformed into Tomato var.Pusa Ruby using Agrobacterium.CP syn was expressed in transgenic tomato plants to avoid gene silencing of the transgene upon virus infection.T 1 transgenic lines were challenged with TYLCV-OM for resistance evaluation.Plants of three transgenic lines out of seven showed resistance response and most plants did not develop disease symptoms.Real-time quantitative PCR showed that the CP syn helped reduce virus particles by 150-fold.Transforming tomato plants with CP syn resulted in the induction of resistance to TYLCV-OM.The transgenic plants are valuable resource to understand the function of the coat protein and to provide resistance against the main begomoviruses infecting tomatoes.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 4
Claim · model-written summary
Three of seven T1 transgenic tomato lines expressing CP syn showed a resistance response to TYLCV-OM.
- Taxon
- tomato
Solanum lycopersicumt0 - Material
- T 1 transgenic linese3
- Treatment
- CP syn was expressed in transgenic tomato plantse1
- Interactor
- Tomato yellow leaf curl virus Oman (TYLCV-OM)e0pathogen
- Gene
- CP syne1
- Outcome
- resistance responsee2
- Value
- three transgenic lines out of sevene2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Expressed in Transgenic Tomato”
e0“the induction of resistance to Tomato yellow leaf curl virus Oman (TYLCV-OM)”
e1“CP syn was expressed in transgenic tomato plants”
e2“Plants of three transgenic lines out of seven showed resistance response and most plants did not develop disease symptoms”
e3“T 1 transgenic lines were challenged with TYLCV-OM for resistance evaluation”
Claim · model-written summary
Most transgenic CP syn tomato plants did not develop disease symptoms after TYLCV-OM challenge.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- CP syn was expressed in transgenic tomato plantse1
- Interactor
- Tomato yellow leaf curl virus Oman (TYLCV-OM)e0pathogen
- Outcome
- disease symptomse2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Expressed in Transgenic Tomato”
e0“the induction of resistance to Tomato yellow leaf curl virus Oman (TYLCV-OM)”
e1“CP syn was expressed in transgenic tomato plants”
e2“Plants of three transgenic lines out of seven showed resistance response and most plants did not develop disease symptoms”
Claim · model-written summary
CP syn reduced TYLCV-OM virus particles in transgenic tomato by 150-fold, as measured by real-time quantitative PCR.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- CP syne1
- Interactor
- Tomato yellow leaf curl virus Oman (TYLCV-OM)e0pathogen
- Outcome
- virus particlese1
- Direction
- reducee1
- Value
- 150-folde1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Expressed in Transgenic Tomato”
e0“the induction of resistance to Tomato yellow leaf curl virus Oman (TYLCV-OM)”
e1“the CP syn helped reduce virus particles by 150-fold”
Claim · model-written summary
Transforming tomato plants with CP syn induced resistance to TYLCV-OM.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Transforming tomato plants with CP syne1
- Interactor
- Tomato yellow leaf curl virus Oman (TYLCV-OM)e0pathogen
- Outcome
- induction of resistancee1
- Direction
- increaseinferrede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Expressed in Transgenic Tomato”
e0“the induction of resistance to Tomato yellow leaf curl virus Oman (TYLCV-OM)”
e1“Transforming tomato plants with CP syn resulted in the induction of resistance to TYLCV-OM”
The same questions in other papers
transgenic · molecular: expression · Solanum lycopersicum (tomato)Positive and negative results
4 papers in the same direction as this paper · 6 in the opposite direction
- no effect
- negative
- negative
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.