Abstract
Stable transformation of tomato (Lycopersicon esculentum cv Ailsa Craig) plants with a construct containing the antisense sequence for the receiver domain and 3'-untranslated portion of the tomato ethylene receptor (LeETR1) under the control of an enhanced cauliflower mosaic virus 35S promoter resulted in some expected and unexpected phenotypes. In addition to reduced LeETR1 transcript levels, the two most consistently observed phenotypes in the transgenic lines were delayed abscission and reduced plant size. Fruit coloration and softening were essentially unaffected, and all the seedlings from first generation seed displayed a normal triple response to ethylene. Two independent lines with a single copy of the transgene and reduced LeETR1 transcript accumulation were selected for detailed phenotypic analysis of second generation (R1) plants. Delayed abscission, shorter internode length, and reduced auxin movement all correlated with the presence of the transgene and the degree of reduced LeETR1 transcript accumulation. No significant differences were noted for fruit coloration or fruit softening on R1 plants and all seedlings from R1 and R2 seed displayed a normal triple response. LeETR2 transcript accumulation was only slightly reduced in the R1 plants compared with azygous plants, and LeETR3 (NR) transcript levels appeared to be unaffected by the transgene. We propose that ethylene signal transduction occurs through parallel paths that partially intersect to regulate shared ethylene responses.
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Claims 11
Claim · model-written summary
Antisense transformation reduced LeETR1 transcript levels.
- Taxon
- Tomato
Solanum lycopersicumt0 - Treatment
- Transgenict0
- Gene
- LeETR1e0
- Outcome
- LeETR1 transcript levelse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In addition to reduced LeETR1 transcript levels”
t0“Transgenic Tomato”
Claim · model-written summary
Transgenic tomato lines showed delayed abscission.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- transgenic linese0
- Outcome
- abscissione0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the two most consistently observed phenotypes in the transgenic lines were delayed abscission and reduced plant size”
t0“Tomato”
Claim · model-written summary
Transgenic tomato lines showed reduced plant size.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- transgenic linese0
- Outcome
- plant sizee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the two most consistently observed phenotypes in the transgenic lines were delayed abscission and reduced plant size”
t0“Tomato”
Claim · model-written summary
In R1 plants, shorter internodes correlated with the transgene and lower LeETR1 accumulation.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- LeETR1e0
- Outcome
- shorter internode lengthe0
- Direction
- correlatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Delayed abscission, shorter internode length, and reduced auxin movement all correlated with the presence of the transgene and the degree of reduced LeETR1 transcript accumulation”
t0“Tomato”
Claim · model-written summary
Reduced auxin movement correlated with transgene presence and lower LeETR1 accumulation.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- LeETR1e0
- Outcome
- auxin movemente0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduced auxin movement all correlated with the presence of the transgene and the degree of reduced LeETR1 transcript accumulation”
t0“Tomato”
Claim · model-written summary
Fruit coloration showed no significant difference in R1 plants.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- R1 plantse0
- Outcome
- fruit coloratione0
- Direction
- No significant differencese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No significant differences were noted for fruit coloration or fruit softening on R1 plants”
t0“Tomato”
Claim · model-written summary
Fruit softening showed no significant difference in R1 plants.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- R1 plantse0
- Outcome
- fruit softeninge0
- Direction
- No significant differencese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No significant differences were noted for fruit coloration or fruit softening on R1 plants”
t0“Tomato”
Claim · model-written summary
R1 and R2 seedlings had a normal ethylene triple response.
- Taxon
- TomatoSolanum lycopersicumt0
- Outcome
- normal triple responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all seedlings from R1 and R2 seed displayed a normal triple response”
t0“Tomato”
Claim · model-written summary
LeETR2 transcript accumulation was slightly lower in R1 than azygous plants.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- LeETR2e0
- Outcome
- LeETR2 transcript accumulatione0
- Direction
- slightly reducede0
- Comparator
- azygous plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LeETR2 transcript accumulation was only slightly reduced in the R1 plants compared with azygous plants”
t0“Tomato”
Claim · model-written summary
LeETR3 transcript levels appeared unaffected by the transgene.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- transgenee0
- Gene
- LeETR3 (NR)e0
- Outcome
- transcript levelse0
- Direction
- unaffectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LeETR3 (NR) transcript levels appeared to be unaffected by the transgene”
t0“Tomato”
Claim · model-written summary
The authors propose partially intersecting parallel ethylene signaling pathways.
- Taxon
- TomatoSolanum lycopersicumt0
- Outcome
- shared ethylene responsese0
- Qualifier
- parallel paths that partially intersecte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We propose that ethylene signal transduction occurs through parallel paths that partially intersect to regulate shared ethylene responses”
t0“Tomato”
The same questions in other papers
transgenic · molecular: expression · Solanum lycopersicum (tomato)Positive and negative results
5 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
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7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.