Claim · model-written summary
HsfA2 plasmid restored transcription factor expression and reporter activation in CS protoplasts.
- Taxon
- tomato
Solanum lycopersicumt0 - Treatment
- transformation with HsfA2 expression plasmide0
- Gene
- HsfA2e0
- Outcome
- normal expression of the transcription factor and reporter gene activatione0
- Direction
- normale0
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Only transformation with HsfA2 expression plasmid led to normal expression of the transcription factor and reporter gene activation”
t0“tomato”
Title
Shravan Kumar Mishra; Joanna Tripp; Sybille Winkelhaus; et al. Genes & Development 2002. doi:10.1101/gad.228802
Other claims from this paper (10)
- Observation HsfA1 overexpressing plants contained one transgene cassette.
- Mechanism Tandem inverted cassette repeats led to HsfA1 cosuppression and small interfering RNAs.
- Observation Developmental parameters were similar among wild type, overexpressing, and cosuppressed plants in normal growth.
- Observation Cosuppressed tomato plants and fruits were highly temperature sensitive.
- Observation Heat stress induced chaperone and Hsf synthesis was reduced or absent in cosuppressed plants.
- Mechanism HsfA1 had a master regulator role in induced thermotolerance.
- Observation Plasmid HsfA1 and HsfA2 were expressed in wild type protoplasts.
- Observation Cosuppression was reproduced in CS protoplasts.
- Observation HsfA1 expression plasmids remained silenced at high amounts.
- Intervention effect Plasmid borne HsfA2 restored thermotolerance, chaperones, luciferase thermoprotection, and heat stress granules in CS protoplasts.