Abstract
A new transgenic technique—— hyperosmosis transformation is studied and explored.Oryza stiva L, Variety TP309 and Lycopersion esculetum,Variety moneymaker are used as the acceptor materials,and pBI121 and pBG are used as the exogenous DNA.The main results are as follows.The calli of rice and tomato kept active after immersed in sucrose (0.75 mg/L) solution for 4 hours,their differentiation is restrained,especially by hypoosmosis treatment following hyperosmosis treatment.The calli of tomato or rice is immerged in a solution containing sucrose or mannitol,plasmid DNA pBI121 or pBG and salmon sperm DNA.4 hours later,CaCl2 or PEG is added to the solution every one hour.Then the treated calli are cultured and identified.The result shows that the growth speed of the calli of tomato obviously decreased after hyperosmosis transformation.The average GUS transient expression is about 38.9%,The GUS transient expression of tomato calli dropped after hyperosmosis transformation mediated by PEG(20%).There are hardly differences in GUS transient expression between calli from seeds and those from spikelets of rice,and between transformations by sucrose solution and by mannitol solution. Some anti-basta calli of rice and tomato and 29 anti-basta buds of tomato are found.A new plant transgenic technique——hyperosmosis transformation is established.
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Claims 7
Claim · model-written summary
Rice and tomato calli stayed active after sucrose immersion, but differentiation was restrained.
- Taxon
- rice
Oryza sativae0 - Treatment
- sucrose (0.75 mg/L) solution for 4 hourse0
- Outcome
- differentiatione0
- Direction
- restrainede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The calli of rice and tomato kept active after immersed in sucrose (0.75 mg/L) solution for 4 hours,their differentiation is restrained”
Claim · model-written summary
Tomato callus growth speed decreased after hyperosmosis transformation.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- hyperosmosis transformatione0
- Outcome
- growth speed of the callie0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“growth speed of the calli of tomato obviously decreased after hyperosmosis transformation”
Claim · model-written summary
Average GUS transient expression was about 38.9%.
Not reportedTaxon
Verbatim
e0“The average GUS transient expression is about 38.9%”
Claim · model-written summary
PEG-mediated transformation lowered GUS transient expression in tomato calli.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- hyperosmosis transformation mediated by PEG(20%)e0
- Outcome
- GUS transient expressione0
- Direction
- droppede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GUS transient expression of tomato calli dropped after hyperosmosis transformation mediated by PEG(20%).”
Claim · model-written summary
GUS transient expression hardly differed between rice calli from seeds and spikelets.
- Taxon
- riceOryza sativae0
- Organ / stage
- seedse0
- Outcome
- GUS transient expressione0
- Direction
- hardly differencese0
- Comparator
- spikeletse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There are hardly differences in GUS transient expression between calli from seeds and those from spikelets of rice”
Claim · model-written summary
GUS transient expression hardly differed between sucrose and mannitol transformation solutions.
Not reportedTaxonDirection
Verbatim
e0“between transformations by sucrose solution and by mannitol solution”
e1“The average GUS transient expression is about 38.9%”
Claim · model-written summary
Anti-basta calli and buds were obtained following transformation.
- Taxon
- tomatoSolanum lycopersicume0
- Outcome
- anti-basta budse0
- Value
- 29e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some anti-basta calli of rice and tomato and 29 anti-basta buds of tomato are found.”
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