Claim · model-written summary
Plasmid HsfA1 and HsfA2 were expressed in wild type protoplasts.
- Taxon
- tomato
Solanum lycopersicume1 - Organ / stage
- mesophyll protoplastse1
- Gene
- HsfA1 and HsfA2e0
- Outcome
- well expressede0
Not reportedMaterialCompoundInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plasmid-encoded HsfA1 and HsfA2 were well expressed”
e1“mesophyll protoplasts from WT tomato”
Title
Shravan Kumar Mishra; Joanna Tripp; Sybille Winkelhaus; et al. Genes & Development 2002. doi:10.1101/gad.228802
Other claims from this paper (10)
- Observation HsfA1 overexpressing plants contained one transgene cassette.
- Mechanism Tandem inverted cassette repeats led to HsfA1 cosuppression and small interfering RNAs.
- Observation Developmental parameters were similar among wild type, overexpressing, and cosuppressed plants in normal growth.
- Observation Cosuppressed tomato plants and fruits were highly temperature sensitive.
- Observation Heat stress induced chaperone and Hsf synthesis was reduced or absent in cosuppressed plants.
- Mechanism HsfA1 had a master regulator role in induced thermotolerance.
- Observation Cosuppression was reproduced in CS protoplasts.
- Intervention effect HsfA2 plasmid restored transcription factor expression and reporter activation in CS protoplasts.
- Observation HsfA1 expression plasmids remained silenced at high amounts.
- Intervention effect Plasmid borne HsfA2 restored thermotolerance, chaperones, luciferase thermoprotection, and heat stress granules in CS protoplasts.