Abstract
Tomato (Solanum lycopersicum) fruit ripening-specific N-glycan processing enzyme, β-D-N-acetylhexosaminidase (β-Hex), plays an important role in the ripening-associated fruit-softening process. However, the regulation of fruit ripening-specific expression of β-Hex is not well understood. We have identified and functionally characterized the fruit ripening-specific promoter of β-Hex and provided insights into its transcriptional regulation during fruit ripening. Our results demonstrate that RIPENING INHIBITOR (RIN), a global fruit ripening regulator, and ABSCISIC ACID STRESS RIPENING 1 (SlASR1), a poorly characterized ripening-related protein, are the transcriptional regulators of β-Hex. Both RIN and SlASR1 directly bound to the β-Hex promoter fragments containing CArG and C₂₋₃(C/G)A cis-acting elements, the binding sites for RIN and SlASR1, respectively. Moreover, β-Hex expression/promoter activity in tomato fruits was downregulated once expression of either RIN or SlASR1 was suppressed; indicating that RIN and SlASR1 positively regulate the transcription of β-Hex during fruit ripening. Interestingly, RIN could also bind to the SlASR1 promoter, which contains several CArG cis-acting elements, and SlASR1 expression was suppressed in rin mutant fruits, indicating that RIN also acts as a positive regulator of SlASR1 expression during fruit ripening. Taken together, these results suggest that RIN, both directly and indirectly, through SlASR1, regulates the transcription of β-Hex during fruit ripening. The fruit ripening-specific promoter of β-Hex could be a useful tool in regulating gene expression during fruit ripening.
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Claims 8
Claim · model-written summary
A fruit ripening-specific β-Hex promoter was identified and characterized.
- Taxon
- Tomato
Solanum lycopersicumt0 - Gene
- β-Hexe0
- Outcome
- fruit ripening-specific promoter of β-Hexe0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have identified and functionally characterized the fruit ripening-specific promoter of β-Hex”
t0“Tomato (Solanum lycopersicum) fruit ripening-specific N-glycan processing enzyme”
Claim · model-written summary
RIN bound β-Hex promoter fragments containing CArG elements.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- RINe0
- Outcome
- bound to the β-Hex promoter fragments containing CArGe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both RIN and SlASR1 directly bound to the β-Hex promoter fragments containing CArG and C₂₋₃(C/G)A cis-acting elements”
t0“Tomato (Solanum lycopersicum) fruit ripening-specific N-glycan processing enzyme”
Claim · model-written summary
SlASR1 bound β-Hex promoter fragments containing C₂₋₃(C/G)A elements.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- SlASR1e0
- Outcome
- bound to the β-Hex promoter fragments containing CArG and C₂₋₃(C/G)A cis-acting elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both RIN and SlASR1 directly bound to the β-Hex promoter fragments containing CArG and C₂₋₃(C/G)A cis-acting elements”
t0“Tomato (Solanum lycopersicum) fruit ripening-specific N-glycan processing enzyme”
Claim · model-written summary
Suppressing RIN downregulated β-Hex expression or promoter activity in tomato fruit.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- expression of either RIN or SlASR1 was suppressede0
- Gene
- RINe0
- Outcome
- β-Hex expression/promoter activitye0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“β-Hex expression/promoter activity in tomato fruits was downregulated once expression of either RIN or SlASR1 was suppressed”
Claim · model-written summary
RIN bound the SlASR1 promoter.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- RINe0
- Outcome
- bind to the SlASR1 promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RIN could also bind to the SlASR1 promoter, which contains several CArG cis-acting elements”
t0“Tomato (Solanum lycopersicum) fruit ripening-specific N-glycan processing enzyme”
Claim · model-written summary
SlASR1 expression was suppressed in rin mutant fruit.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- rin mutante0
- Gene
- SlASR1e0
- Outcome
- SlASR1 expressione0
- Direction
- suppressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlASR1 expression was suppressed in rin mutant fruits”
t0“Tomato (Solanum lycopersicum) fruit ripening-specific N-glycan processing enzyme”
Claim · model-written summary
RIN positively regulates β-Hex transcription directly and through SlASR1 during fruit ripening.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- RINe0
- Outcome
- transcription of β-Hexe0
- Qualifier
- through SlASR1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RIN, both directly and indirectly, through SlASR1, regulates the transcription of β-Hex during fruit ripening”
t0“Tomato (Solanum lycopersicum) fruit ripening-specific N-glycan processing enzyme”
Claim · model-written summary
The β-Hex fruit ripening promoter may be useful for regulating gene expression during ripening.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- β-Hexe0
- Outcome
- regulating gene expression during fruit ripeninge0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fruit ripening-specific promoter of β-Hex could be a useful tool in regulating gene expression during fruit ripening”
t0“Tomato (Solanum lycopersicum) fruit ripening-specific N-glycan processing enzyme”
The same questions in other papers
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- positive
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See all 11 papers →stage · molecular: expression · Solanum lycopersicum (tomato)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.