Paper · 2021 · Frontiers in Plant Science

Clonal Propagation of Valeriana jatamansi Retains the Essential Oil Profile of Mother Plants: An Approach Toward Generating Homogenous Grade of Essential Oil for Industrial Use

Rahul Dev Gautam; Ajay Kumar; Ravi Kumar; et al.

5
claims
9
verbatim quotes
18
citations
4
research areas
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Open access · cc-by

Abstract

Valeriana jatamansi Jones (Syn. V. wallichii DC.) is an aromatic, medicinal herb used as a tranquilizer and in treating sleep disorders. Rhizome is mainly used to extract essential oil (EO) and valepotriates. High quality and economic yield of rhizomes are available in the third year of growth. Therefore, the cultivation of V. jatamansi is not picking up, and over-exploitation of this plant from wild habitats to meet the increasing demand of the pharmaceutical industry is the cause of threat to the genetic diversity of the species. Further, collections from the wild are heterogeneous, resulting in variable produce. The development of clonal lines can ensure uniform quality and yield of rhizome biomass. An effective clonal propagation method was standardized using different hormonal concentrations of naphthalene acetic acid (NAA) on apical shoot cuttings from the selected clone CSIR-IHBT-VJ-05 for different time durations and raised over various planting media. NAA treatment of 50 ppm concentration for 30 min was found optimum for root induction in apical shoots of V. jatamansi . Variations for EO composition within the clone were non-significant, while samples of the control population were variable. The best quality EO (patchouli alcohol ∼62%) was available during the third year of plant growth. A propagation technique for large-scale quality plant material (QPM) production has been standardized to reduce the stress over natural resources and promote V. jatamansi for use in the aromatic and pharmaceutical industry.

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Claims 5

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

NAA at 50 ppm for 30 minutes was optimal for rooting apical shoots.

Taxon
V. jatamansiValeriana jatamansie0
Organ / stage
apical shootse0
Treatment
NAA treatment of 50 ppm concentration for 30 mine0
Outcome
root inductione0
Value
50 ppme0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Valeriana jatamansi
Valeriana jatamansi · TaxonDaderot · Public domain · Wikimedia Commons ↗

Verbatim

e0

“NAA treatment of 50 ppm concentration for 30 min was found optimum for root induction in apical shoots of V. jatamansi”

✓ located verbatim in the source · abstract, characters 975–1093 · show in text ↑
Observationobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Essential oil composition varied nonsignificantly within the clone.

Taxon
Valeriana jatamansiValeriana jatamansit0
Outcome
EO compositione0
Direction
non-significante0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Valeriana jatamansi
Valeriana jatamansi · TaxonDaderot · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Variations for EO composition within the clone were non-significant”

✓ located verbatim in the source · abstract, characters 1096–1163 · show in text ↑
t0

“Valeriana jatamansi”

✓ located verbatim in the source · title, characters 22–41 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Control population essential oil composition was variable.

Taxon
Valeriana jatamansiValeriana jatamansit0
Material
control populatione0
Outcome
variablee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Valeriana jatamansi
Valeriana jatamansi · TaxonDaderot · Public domain · Wikimedia Commons ↗

Verbatim

e0

“samples of the control population were variable”

✓ located verbatim in the source · abstract, characters 1171–1218 · show in text ↑
t0

“Valeriana jatamansi”

✓ located verbatim in the source · title, characters 22–41 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Best essential oil quality occurred in year three with about 62% patchouli alcohol.

Taxon
Valeriana jatamansiValeriana jatamansit0
Outcome
best quality EOe0
Value
∼62%e0
Qualifier
third year of plant growthe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Valeriana jatamansi
Valeriana jatamansi · TaxonDaderot · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The best quality EO (patchouli alcohol ∼62%) was available during the third year of plant growth”

✓ located verbatim in the source · abstract, characters 1220–1316 · show in text ↑
t0

“Valeriana jatamansi”

✓ located verbatim in the source · title, characters 22–41 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

A propagation technique for large-scale quality plant material production was standardized.

Taxon
Valeriana jatamansiValeriana jatamansit0
Outcome
propagation technique for large-scale quality plant material (QPM) productione0
Value
large-scalee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Valeriana jatamansi
Valeriana jatamansi · TaxonDaderot · Public domain · Wikimedia Commons ↗

Verbatim

e0

“A propagation technique for large-scale quality plant material (QPM) production has been standardized”

✓ located verbatim in the source · abstract, characters 1318–1419 · show in text ↑
t0

“Valeriana jatamansi”

✓ located verbatim in the source · title, characters 22–41 · show in text ↑