Abstract
Symbiotic nodulation is initiated in the roots of legumes in response to low nitrogen and rhizobial signal molecules and is dynamically regulated by a complex regulatory network that coordinates rhizobial infection and nodule organogenesis. It has been shown that the miR156-SPL module mediates nodulation in legumes; however, conclusive evidence of how this module exerts its function during nodulation remains elusive. Here, we report that the miR156b-GmSPL9d module regulates symbiotic nodulation by targeting multiple key regulatory genes in the nodulation signalling pathway of soybean. miR156 family members are differentially expressed during nodulation, and miR156b negatively regulates nodulation by mainly targeting soybean SQUAMOSA promoter-binding protein-like 9d (GmSPL9d), a positive regulator of soybean nodulation. GmSPL9d directly binds to the miR172c promoter and activates its expression, suggesting a conserved role of GmSPL9d. Furthermore, GmSPL9d was coexpressed with the soybean nodulation marker genes nodule inception a (GmNINa) and GmENOD40-1 during nodule formation and development. Intriguingly, GmSPL9d can bind to the promoters of GmNINa and GmENOD40-1 and regulate their expression. Our data demonstrate that the miR156b-GmSPL9d module acts as an upstream master regulator of soybean nodulation, which coordinates multiple marker genes involved in soybean nodulation.
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Claims 6
Claim · model-written summary
miR156 family members are differentially expressed during nodulation in soybean.
- Taxon
- soybean
Glycine maxt0 - Organ / stage
- nodulatione0
- Gene
- miR156e0
- Outcome
- differentially expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR156 family members are differentially expressed during nodulation”
t0“soybean”
Claim · model-written summary
miR156b negatively regulates nodulation, mainly by targeting soybean GmSPL9d.
- Taxon
- soybeanGlycine maxe0
- Gene
- miR156be0
- Outcome
- nodulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR156b negatively regulates nodulation by mainly targeting soybean SQUAMOSA promoter-binding protein-like 9d (GmSPL9d)”
Claim · model-written summary
GmSPL9d is a positive regulator of soybean nodulation.
- Taxon
- soybeanGlycine maxe0
- Gene
- GmSPL9de0
- Outcome
- nodulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“(GmSPL9d), a positive regulator of soybean nodulation”
Claim · model-written summary
GmSPL9d directly binds to the miR172c promoter and activates its expression.
- Taxon
- soybeanGlycine maxt0
- Gene
- GmSPL9de0
- Outcome
- binds to the miR172c promoter and activates its expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmSPL9d directly binds to the miR172c promoter and activates its expression”
t0“soybean”
Claim · model-written summary
GmSPL9d was coexpressed with soybean nodulation marker genes GmNINa and GmENOD40-1 during nodule formation and development.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- nodule formation and developmente0
- Gene
- GmSPL9de0
- Outcome
- coexpressede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmSPL9d was coexpressed with the soybean nodulation marker genes nodule inception a (GmNINa) and GmENOD40-1 during nodule formation and development”
Claim · model-written summary
GmSPL9d can bind to the promoters of GmNINa and GmENOD40-1 and regulate their expression.
- Taxon
- soybeanGlycine maxt0
- Gene
- GmSPL9de0
- Outcome
- bind to the promoters of GmNINa and GmENOD40-1 and regulate their expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmSPL9d can bind to the promoters of GmNINa and GmENOD40-1 and regulate their expression”
t0“soybean”