Abstract
Abstract Background Ralstonia solanacearum , one of the most devastating bacterial plant pathogens, is the causal agent of bacterial wilt. Recently, several studies on resistance to bacterial wilt have been conducted using the Arabidopsis- R. solanacearum system. However, the progress of R. solanacearum infection in Arabidopsis is still unclear. Results We generated a bioluminescent R. solanacearum by expressing plasmid-based lux CDABE. Expression of lux CDABE did not alter the bacterial growth and pathogenicity. The light intensity of bioluminescent R. solanacearum was linearly related to bacterial concentrations from 10 4 to 10 8 CFU·mL −1 . After root inoculation with bioluminescent R. solanacearum strain, light signals in tomato and Arabidopsis were found to be transported from roots to stems via the vasculature. Quantification of light intensity from the bioluminescent strain accurately reported the difference in disease resistance between Arabidopsis wild type and resistant mutants. Conclusions Bioluminescent R. solanacearum strain spatially and quantitatively measured bacterial growth in tomato and Arabidopsis, and offered a tool for the high-throughput study of R. solanacearum -Arabidopsis interaction in the future.
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Claims 4
Claim · model-written summary
Light intensity of bioluminescent R. solanacearum was linearly related to bacterial concentrations from 10 4 to 10 8 CFU·mL −1.
- Taxon
- tomato
Solanum lycopersicumt0 - Interactor
- R. solanacearum
Ralstonia solanacearume0pathogen - Outcome
- light intensitye0
- Value
- 10 4 to 10 8 CFU·mL −1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“The light intensity of bioluminescent R. solanacearum was linearly related to bacterial concentrations from 10 4 to 10 8 CFU·mL −1”
Claim · model-written summary
After root inoculation, bioluminescent light signals of R. solanacearum were transported from roots to stems via the vasculature in tomato and Arabidopsis.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- roots to stemse1
- Treatment
- root inoculation with bioluminescent R. solanacearum straine1
- Interactor
- R. solanacearumRalstonia solanacearume1pathogen
- Outcome
- light signalse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e1“After root inoculation with bioluminescent R. solanacearum strain, light signals in tomato and Arabidopsis were found to be transported from roots to stems via the vasculature”
Claim · model-written summary
Quantified light intensity reported the difference in disease resistance between Arabidopsis wild type and resistant mutants.
- Taxon
- Arabidopsis
Arabidopsist1 - Material
- resistant mutantse2
- Outcome
- difference in disease resistancee2
- Comparator
- wild typee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Arabidopsis”
e2“Quantification of light intensity from the bioluminescent strain accurately reported the difference in disease resistance between Arabidopsis wild type and resistant mutants”
Claim · model-written summary
Expression of lux CDABE did not alter the growth or pathogenicity of the bacterium on tomato.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Expression of lux CDABEe3
- Outcome
- bacterial growth and pathogenicitye3
- Direction
- did not altere3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e3“Expression of lux CDABE did not alter the bacterial growth and pathogenicity”
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