Abstract
In date palm micropropagation protocols, the female inflorescences play an important role, being available tissue culture-explants, alternatively of the limited number of renewing offshoots explants of date palm that used for in vitro propagation. This study conducted to investigate the effect of different auxins types (2,4-D, NAA, NOA) at different concentrations, supplemented in induction culture medium of constant concentration of cytokinins, on inducing the direct organogenesis by adventitious shoots differentiation, of the date palm immature female inflorescences explants, of Barhe cultivar. Also, the interaction effect of slicing method (for the first time studying) of the starting spikelet explants was studied. The results showed that, the induction medium of the appropriate ratio of the NAA auxin type to cytokinins of induction medium, with applying the slicing method of fine chopping for the cultured spikelet, showed the most satisfying results, of direct adventitious shoots formation, direct somatic embryos, and less formation of direct adventitious roots. Developing the date palm micropropagation protocol by inflorescences explants considered a reliable and rapid opportunity to avoid the shortage of date palm offshoots and covering the commercial demands on the date palm plantlets.
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Claims 4
Claim · model-written summary
NAA-to-cytokinin ratio with fine chopping favored direct adventitious shoot formation.
- Treatment
- appropriate ratio of the NAA auxin type to cytokinins of induction medium, with applying the slicing method of fine choppinge0
- Outcome
- direct adventitious shoots formatione0
- Direction
- most satisfyinge0
Not reportedTaxonComparator
Verbatim
e0“the induction medium of the appropriate ratio of the NAA auxin type to cytokinins of induction medium, with applying the slicing method of fine chopping for the cultured spikelet, showed the most satisfying results, of direct adventitious shoots formation”
Claim · model-written summary
The same treatment favored direct somatic embryos.
- Outcome
- direct somatic embryose0
Not reportedTaxonTreatmentComparatorDirection
Verbatim
e0“direct somatic embryos”
Claim · model-written summary
The same treatment produced less direct adventitious root formation.
Not reportedTaxonTreatmentComparator
Verbatim
e0“less formation of direct adventitious roots”
Claim · model-written summary
Authors considered inflorescence micropropagation a rapid way to address offshoot shortage and plantlet demand.
- Taxon
- date palm
Phoenix dactyliferae0 - Organ / stage
- inflorescences explantse0
- Outcome
- reliable and rapid opportunitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Developing the date palm micropropagation protocol by inflorescences explants considered a reliable and rapid opportunity to avoid the shortage of date palm offshoots and covering the commercial demands on the date palm plantlets.”