Paper · 2022 · Agronomy

16S rRNA Gene-Based Metagenomic Analysis of Rhizosphere Soil Bacteria in Arkansas Rice Crop Fields

Cássia Oliveira; Ehsan Shakiba; Dustin North; et al.

5
claims
10
verbatim quotes
21
citations
2
research areas
✓
Open access · cc-by

Abstract

The rhizomicrobiome is composed of microbes that live in association with plant roots. From nutrient cycling to carbon sequestration, soil microorganisms have provided a solid base for natural and agricultural ecosystems to function. The relationship between plant roots and soil microorganisms is especially relevant in food staples such as rice (Oryza sativa L.), as the various properties of these microbes can influence crop yield and plant health, thereby affecting a major portion of the food supply for an ever-growing world population. In this study, we used 16S rRNA gene-based metagenomic analysis to investigate the impact of crop rotation and soil cultivation methods (no-till or tillage) on rhizosphere bacterial diversity and composition in eight crop fields in Arkansas. Illumina MiSeq sequencing revealed 56 Phyla, with four major Phyla: Proteobacteria, Acidobacteria, Actinobacteria, and Bacteroidetes. Soil microbial communities in the samples studied were phylogenetically diverse but with a stable community structure. Crop rotation and tillage did not significantly affect bacterial diversity.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 5

Observationobservedoriginal resultmeasured

Claim · model-written summary

Sequencing identified 56 bacterial phyla in rice rhizosphere samples.

Outcome
Phylae0
Value
56 Phylae0
Setting
Arkansast0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Illumina MiSeq sequencing revealed 56 Phyla, with four major Phyla: Proteobacteria, Acidobacteria, Actinobacteria, and Bacteroidetes”

✓ located verbatim in the source · abstract, characters 786–918 · show in text ↑
t0

“Rhizosphere Soil Bacteria in Arkansas Rice Crop Fields”

✓ located verbatim in the source · title, characters 44–98 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Proteobacteria, Acidobacteria, Actinobacteria and Bacteroidetes were the four major phyla.

Outcome
Proteobacteria, Acidobacteria, Actinobacteria, and Bacteroidetese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“four major Phyla: Proteobacteria, Acidobacteria, Actinobacteria, and Bacteroidetes”

✓ located verbatim in the source · abstract, characters 836–918 · show in text ↑
t0

“Rhizosphere Soil Bacteria in Arkansas Rice Crop Fields”

✓ located verbatim in the source · title, characters 44–98 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Sampled soil microbial communities were phylogenetically diverse but structurally stable.

Outcome
phylogenetically diverse but with a stable community structuree0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Soil microbial communities in the samples studied were phylogenetically diverse but with a stable community structure”

✓ located verbatim in the source · abstract, characters 920–1037 · show in text ↑
t0

“Rice Crop Fields”

✓ located verbatim in the source · title, characters 82–98 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Crop rotation did not significantly affect rice rhizosphere bacterial diversity.

Treatment
Crop rotatione0
Outcome
bacterial diversitye0
Direction
did not significantly affecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Crop rotation and tillage did not significantly affect bacterial diversity”

✓ located verbatim in the source · abstract, characters 1039–1113 · show in text ↑
t0

“Rice Crop Fields”

✓ located verbatim in the source · title, characters 82–98 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Tillage did not significantly affect rice rhizosphere bacterial diversity.

Treatment
tillagee0
Outcome
bacterial diversitye0
Direction
did not significantly affecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Crop rotation and tillage did not significantly affect bacterial diversity”

✓ located verbatim in the source · abstract, characters 1039–1113 · show in text ↑
t0

“Rice Crop Fields”

✓ located verbatim in the source · title, characters 82–98 · show in text ↑

rotation · soil: biology · Oryza sativa (rice)Positive and negative results

5 papers: 4 positive, 1 negative or no effect

4 in the opposite direction

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

tillage · soil: biology · Oryza sativa (rice)Positive and negative results

16 papers: 13 positive, 3 negative or no effect

2 papers in the same direction as this paper · 13 in the opposite direction

  1. positive
  2. positive
  3. positive

12 papers more in this group

See all 16 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.