Extraction, Evaluation, and Antioxidant Activity of Total Phenol from Callus of Abutilon indicum (L.) Sweet

Abstract

In this present study, callus induction from the leaves of Abutilon indicum was performed and evaluated antioxidant potential after TLC, PTLC. For induction of callus, Murashige, and Skoog (MS) medium with 2.5 mg/L concentration of 2, 4 Dichloro phenoxy acetic acid (2,4-D) and for assay, Folin Ciocalteau method practiced for evaluation of total phenol, modified spectrophotometer method used for DPPH assay. Based on total phenolic content, total antioxidant capacity, DPPH, assay, and reducing power assay performed after separation and partial purification of total phenols by Thin-layer chromatography (TLC) and Preparative thin-layer chromatography (PTLC), respectively. For total phenol estimation, hot water extract shows the highest total phenol content (10.56 μg/ml) by comparing another extract; similarly, PTLC fraction of callus extract from Abutilon indicum shows 61% of inhibition in DPPH at 10 μg/ml. From this study, compared to leaf, callus shows a higher concentration of total phenol and shows promising antioxidant activity. So, it gives scope for further exploration and purification and application of total phenol from callus of Abutilon indicum.

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