Paper · 2021 · Advances in Horticultural Science

Effect of a double phase culture system and activated charcoal on in vitro propagation of Malus sylvestris (L.) Mill.

Valbona Sota; Carla Benelli; Brunilda Çuko; et al.

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claims
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verbatim quotes
10
citations
4
research areas
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Open access · cc-by

Abstract

The effectiveness of a double phase (solid/liquid) culture system (DPS) in comparison to a conventional (solid) system (CS) as well as, the role of various concentrations of activated charcoal in both systems on the enhancement of micropropagation of Malus sylvestris (L.) Mill. were investigated. In this study, lateral shoots were used as primary explants and a comparison for shoots regeneration and rooting abilities was assessed between DPS and CS micropropagation systems. Also, the effect of activated charcoal concentration (0, 250, 500, 1000 mg l-­1) during rooting stage was evaluated for both micropropagation systems. All assessed biometric parameters were higher in the DPS propagation system. The addition of activated charcoal induced effectively rhizogenesis in both systems, whereas the highest value of roots length (13.16 cm) was in the DPS system supplemented with activated charcoal at 500 mg l-­1. The DPS culture system represents a promising low-­cost and time­-saving technique which may improve micropropagation efficiency in producing a large quantity of homogenous wild apple plants.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

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Claims 4

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

All biometric parameters were higher in the double phase system.

Taxon
Malus sylvestris (L.) Mill.Malus sylvestrist0
Treatment
DPS propagation systeme0
Outcome
assessed biometric parameterse0
Direction
highere0
Comparator
conventional (solid) system (CS)e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus sylvestris
Malus sylvestris · TaxonPer Arvid Åsen · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“All assessed biometric parameters were higher in the DPS propagation system”

✓ located verbatim in the source · abstract, characters 630–705 · show in text ↑
t0

“Malus sylvestris (L.) Mill.”

✓ located verbatim in the source · title, characters 90–117 · show in text ↑
t1

“Malus sylvestris (L.) Mill.”

✓ located verbatim in the source · title, characters 90–117 · show in text ↑
e1

“conventional (solid) system (CS)”

✓ located verbatim in the source · abstract, characters 91–123 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Activated charcoal induced rhizogenesis in both systems.

Taxon
Malus sylvestris (L.) Mill.Malus sylvestrist0
Treatment
activated charcoale0
Outcome
rhizogenesise0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus sylvestris
Malus sylvestris · TaxonPer Arvid Åsen · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The addition of activated charcoal induced effectively rhizogenesis in both systems”

✓ located verbatim in the source · abstract, characters 707–790 · show in text ↑
t0

“Malus sylvestris (L.) Mill.”

✓ located verbatim in the source · title, characters 90–117 · show in text ↑
t1

“Malus sylvestris (L.) Mill.”

✓ located verbatim in the source · title, characters 90–117 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The double phase system with 500 mg l-­1 activated charcoal had maximum root length of 13.16 cm.

Taxon
Malus sylvestris (L.) Mill.Malus sylvestrist0
Treatment
activated charcoal at 500 mg l-­1e0
Outcome
roots lengthe0
Direction
higheste0
Value
13.16 cme0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus sylvestris
Malus sylvestris · TaxonPer Arvid Åsen · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the highest value of roots length (13.16 cm) was in the DPS system supplemented with activated charcoal at 500 mg l-­1”

✓ located verbatim in the source · abstract, characters 800–918 · show in text ↑
t0

“Malus sylvestris (L.) Mill.”

✓ located verbatim in the source · title, characters 90–117 · show in text ↑
t1

“Malus sylvestris (L.) Mill.”

✓ located verbatim in the source · title, characters 90–117 · show in text ↑
Method / resourceauthor interpretationoriginal resultmeasured

Claim · model-written summary

The authors considered DPS a low-cost and time-saving technique for micropropagation.

Taxon
Malus sylvestris (L.) Mill.Malus sylvestrist0
Outcome
low-­cost and time­-saving techniquee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus sylvestris
Malus sylvestris · TaxonPer Arvid Åsen · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The DPS culture system represents a promising low-­cost and time­-saving technique which may improve micropropagation efficiency”

✓ located verbatim in the source · abstract, characters 920–1048 · show in text ↑
t0

“Malus sylvestris (L.) Mill.”

✓ located verbatim in the source · title, characters 90–117 · show in text ↑
t1

“Malus sylvestris (L.) Mill.”

✓ located verbatim in the source · title, characters 90–117 · show in text ↑