Abstract
The effectiveness of a double phase (solid/liquid) culture system (DPS) in comparison to a conventional (solid) system (CS) as well as, the role of various concentrations of activated charcoal in both systems on the enhancement of micropropagation of Malus sylvestris (L.) Mill. were investigated. In this study, lateral shoots were used as primary explants and a comparison for shoots regeneration and rooting abilities was assessed between DPS and CS micropropagation systems. Also, the effect of activated charcoal concentration (0, 250, 500, 1000 mg l-1) during rooting stage was evaluated for both micropropagation systems. All assessed biometric parameters were higher in the DPS propagation system. The addition of activated charcoal induced effectively rhizogenesis in both systems, whereas the highest value of roots length (13.16 cm) was in the DPS system supplemented with activated charcoal at 500 mg l-1. The DPS culture system represents a promising low-cost and time-saving technique which may improve micropropagation efficiency in producing a large quantity of homogenous wild apple plants.
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Claims 4
Claim · model-written summary
All biometric parameters were higher in the double phase system.
- Taxon
- Malus sylvestris (L.) Mill.
Malus sylvestrist0 - Treatment
- DPS propagation systeme0
- Outcome
- assessed biometric parameterse0
- Direction
- highere0
- Comparator
- conventional (solid) system (CS)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tre.jpg?width=500)
Verbatim
e0“All assessed biometric parameters were higher in the DPS propagation system”
t0“Malus sylvestris (L.) Mill.”
t1“Malus sylvestris (L.) Mill.”
e1“conventional (solid) system (CS)”
Claim · model-written summary
Activated charcoal induced rhizogenesis in both systems.
- Taxon
- Malus sylvestris (L.) Mill.
Malus sylvestrist0 - Treatment
- activated charcoale0
- Outcome
- rhizogenesise0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tre.jpg?width=500)
Verbatim
e0“The addition of activated charcoal induced effectively rhizogenesis in both systems”
t0“Malus sylvestris (L.) Mill.”
t1“Malus sylvestris (L.) Mill.”
Claim · model-written summary
The double phase system with 500 mg l-1 activated charcoal had maximum root length of 13.16 cm.
- Taxon
- Malus sylvestris (L.) Mill.
Malus sylvestrist0 - Treatment
- activated charcoal at 500 mg l-1e0
- Outcome
- roots lengthe0
- Direction
- higheste0
- Value
- 13.16 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tre.jpg?width=500)
Verbatim
e0“the highest value of roots length (13.16 cm) was in the DPS system supplemented with activated charcoal at 500 mg l-1”
t0“Malus sylvestris (L.) Mill.”
t1“Malus sylvestris (L.) Mill.”
Claim · model-written summary
The authors considered DPS a low-cost and time-saving technique for micropropagation.
- Taxon
- Malus sylvestris (L.) Mill.
Malus sylvestrist0 - Outcome
- low-cost and time-saving techniquee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tre.jpg?width=500)
Verbatim
e0“The DPS culture system represents a promising low-cost and time-saving technique which may improve micropropagation efficiency”
t0“Malus sylvestris (L.) Mill.”
t1“Malus sylvestris (L.) Mill.”