Abstract
Carbenicillin and cefotaxime, two antibiotics routinely employed to exclude Agrobacterium tumefaciens during plant transformation, were studied for their effects on callus growth and the formation of somatic embryos. Antibiotics were tested to see how they responded to the regeneration of Malaysian indica rice cv. MR219. In a preliminary study, callus fresh weight gain appeared to be improved in media containing 200 mg/L carbenicillin and 300-400 mg/L cefotaxime but was severely hindered in media containing higher concentrations. Somatic embryos were discovered in greater numbers in the medium containing 200 mgl-1 carbenicillin or 300 mgl-1 cefotaxime, at 76 and 71, respectively. It was possible that these antibiotics showed a positive response on somatic embryos development when compared to those without antibiotics. Carbenicillin and cefotaxime concentrations greater than 300 mg/L should not be utilized for the process
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Claims 6
Claim · model-written summary
Carbenicillin at 200 mg/L improved rice callus fresh weight gain.
- Taxon
- rice
Oryza sativae0 - Material
- MR219e0
- Treatment
- 200 mg/L carbenicilline1
- Outcome
- callus fresh weight gaine1
- Direction
- improvede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malaysian indica rice cv. MR219”
e1“callus fresh weight gain appeared to be improved in media containing 200 mg/L carbenicillin”
Claim · model-written summary
Cefotaxime at 300-400 mg/L improved rice callus fresh weight gain.
- Taxon
- riceOryza sativae0
- Material
- MR219e0
- Treatment
- 300-400 mg/L cefotaximee1
- Outcome
- callus fresh weight gaine1
- Direction
- improvede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malaysian indica rice cv. MR219”
e1“callus fresh weight gain appeared to be improved in media containing 200 mg/L carbenicillin and 300-400 mg/L cefotaxime”
Claim · model-written summary
Higher antibiotic concentrations severely hindered callus fresh weight gain.
- Taxon
- riceOryza sativae0
- Treatment
- higher concentrationse1
- Outcome
- callus fresh weight gaine1
- Direction
- hinderede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malaysian indica rice cv. MR219”
e1“callus fresh weight gain appeared to be improved in media containing 200 mg/L carbenicillin and 300-400 mg/L cefotaxime but was severely hindered in media containing higher concentrations”
Claim · model-written summary
The 200 mgl-1 carbenicillin medium yielded 76 somatic embryos, more than media without antibiotics.
- Taxon
- riceOryza sativae0
- Treatment
- 200 mgl-1 carbenicilline1
- Outcome
- Somatic embryose1
- Direction
- greater numberse1
- Value
- 76e1
- Comparator
- those without antibioticse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malaysian indica rice cv. MR219”
e1“Somatic embryos were discovered in greater numbers in the medium containing 200 mgl-1 carbenicillin or 300 mgl-1 cefotaxime, at 76 and 71, respectively.”
e2“when compared to those without antibiotics”
Claim · model-written summary
The 300 mgl-1 cefotaxime medium yielded 71 somatic embryos, more than media without antibiotics.
- Taxon
- riceOryza sativae0
- Treatment
- 300 mgl-1 cefotaximee1
- Outcome
- Somatic embryose1
- Direction
- greater numberse1
- Value
- 71e1
- Comparator
- those without antibioticse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malaysian indica rice cv. MR219”
e1“Somatic embryos were discovered in greater numbers in the medium containing 200 mgl-1 carbenicillin or 300 mgl-1 cefotaxime, at 76 and 71, respectively.”
e2“when compared to those without antibiotics”
Claim · model-written summary
The authors recommend avoiding carbenicillin and cefotaxime concentrations above 300 mg/L.
- Taxon
- riceOryza sativae0
- Treatment
- Carbenicillin and cefotaxime concentrations greater than 300 mg/Le1
- Outcome
- should not be utilized for the processe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malaysian indica rice cv. MR219”
e1“Carbenicillin and cefotaxime concentrations greater than 300 mg/L should not be utilized for the process”
The same questions in other papers
cefotaxime · growth · Oryza sativa (rice)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.