Abstract
An antibiotic, cefotaxime (Omnatax) has been found to promote somatic embryogenesis and subsequent plant regeneration in vitro in indica-type basmati rice cultures. Response was highly genotype specific. The number, mass and morphology of the calli formed on the scutellar tissues were dependent on the growth medium (with or without cefotaxime). The embryogenic nature of nodular calli was confirmed through histological analysis and their plant regeneration ability. The calli of variety Pusa basmati 1 grown on medium supplemented with cefotaxime (100 mg/L) exhibited up to 70.5% plant regeneration as compared to control (51.51%). Plants regenerated from emryogenic calli were phenotypically normal and identical to seed-derived plants and exhibited normal fertility. A limited humidity and an optimal aeration of the culture tubes further enhanced the frequency of somatic embryogenesis and plant regeneration.
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Claims 9
Claim · model-written summary
Cefotaxime promotes somatic embryogenesis and subsequent plant regeneration in vitro in indica-type basmati rice cultures.
- Taxon
- indica-type basmati ricee0
- Treatment
- cefotaxime (Omnatax)e0
- Outcome
- somatic embryogenesis and subsequent plant regeneratione0
- Direction
- promotee0
- Setting
- in vitroe0
Not reportedComparator
Verbatim
e0“An antibiotic, cefotaxime (Omnatax) has been found to promote somatic embryogenesis and subsequent plant regeneration in vitro in indica-type basmati rice cultures”
Claim · model-written summary
Calli of Pusa basmati 1 on cefotaxime (100 mg/L) medium showed up to 70.5% plant regeneration, compared with 51.51% for control.
- Taxon
- rice
Oryza sativat0 - Material
- Pusa basmati 1e1
- Organ / stage
- callie1
- Treatment
- cefotaxime (100 mg/L)e1
- Outcome
- plant regeneratione1
- Direction
- higherinferrede1
- Value
- 70.5%e1
- Qualifier
- 51.51%e1
- Comparator
- controle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The calli of variety Pusa basmati 1 grown on medium supplemented with cefotaxime (100 mg/L) exhibited up to 70.5% plant regeneration as compared to control (51.51%)”
t0“indica rice”
Claim · model-written summary
The response of rice in this embryogenesis system was highly genotype specific.
- Taxon
- riceOryza sativat0
- Outcome
- Responsee2
- Qualifier
- highly genotype specifice2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Response was highly genotype specific”
t0“indica rice”
Claim · model-written summary
Number, mass and morphology of calli on scutellar tissues depended on the growth medium, with or without cefotaxime.
- Taxon
- riceOryza sativat0
- Organ / stage
- scutellar tissuese3
- Treatment
- growth medium (with or without cefotaxime)e3
- Outcome
- number, mass and morphology of the callie3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“The number, mass and morphology of the calli formed on the scutellar tissues were dependent on the growth medium (with or without cefotaxime)”
t0“indica rice”
Claim · model-written summary
The embryogenic nature of nodular calli was confirmed by histological analysis.
- Taxon
- riceOryza sativat0
- Outcome
- embryogenic nature of nodular callie4
- Qualifier
- through histological analysise4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“The embryogenic nature of nodular calli was confirmed through histological analysis and their plant regeneration ability”
t0“indica rice”
Claim · model-written summary
The plant regeneration ability of nodular calli was confirmed.
- Taxon
- riceOryza sativat0
- Organ / stage
- nodular callie4
- Outcome
- plant regeneration abilitye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“The embryogenic nature of nodular calli was confirmed through histological analysis and their plant regeneration ability”
t0“indica rice”
Claim · model-written summary
Plants regenerated from embryogenic calli were phenotypically normal and identical to seed-derived plants.
- Taxon
- riceOryza sativat0
- Organ / stage
- callie5
- Outcome
- phenotypically normale5
- Comparator
- seed-derived plantse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Plants regenerated from emryogenic calli were phenotypically normal and identical to seed-derived plants”
t0“indica rice”
Claim · model-written summary
Regenerated plants exhibited normal fertility.
- Taxon
- riceOryza sativat0
- Outcome
- fertilitye6
- Qualifier
- normale6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“exhibited normal fertility”
t0“indica rice”
Claim · model-written summary
A limited humidity and an optimal aeration of the culture tubes further enhanced the frequency of somatic embryogenesis and plant regeneration.
- Taxon
- riceOryza sativat0
- Treatment
- limited humidity and an optimal aeration of the culture tubese7
- Outcome
- frequency of somatic embryogenesis and plant regeneratione7
- Direction
- enhancede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“A limited humidity and an optimal aeration of the culture tubes further enhanced the frequency of somatic embryogenesis and plant regeneration”
t0“indica rice”
The same questions in other papers
cefotaxime · growth · Oryza sativa (rice)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
humidity · growth · Oryza sativa (rice)Positive and negative results
4 papers in the same direction as this paper · 8 in the opposite direction · 1 reporting no difference from an alternative
- negative
- no effect
- no effect
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.