Abstract
During somatic embryogenesis (SE), somatic cells initiate embryogenic development under appropriate conditions. Uniconazole, a plant growth regulator, was found to inhibit the proliferation of callus but promoted the conversion of callus into an embryogenic callus (EC) in cotton. The supplementation of uniconazole in the culture medium significantly suppressed the endogenous auxin [indole acetic acid (IAA)] level in callus tissues in both the callus initiation and proliferation stage but enhanced the abscisic acid (ABA) level only in the callus proliferation stage. Exogenous ABA and uniconazole showed cooperative effects on promoting the differentiation rate of callus into EC. These findings were verified by RNA-seq analysis, which elucidated that the genes involved in the IAA biosynthesis, metabolism, and signaling, and ABA metabolism pathways were regulated by uniconazole during the callus development and SE. Overall, the results suggest that uniconazole could modulate callus proliferation and callus differentiation rate by regulating the endogenous levels of IAA and ABA.
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Claims 7
Claim · model-written summary
Uniconazole inhibited cotton callus proliferation.
- Taxon
- Cotton (Gossypium hirsutum L.)
Gossypium hirsutumt0 - Treatment
- Uniconazolee0
- Outcome
- proliferation of calluse0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Uniconazole, a plant growth regulator, was found to inhibit the proliferation of callus”
t0“Cotton (Gossypium hirsutum L.)”
Claim · model-written summary
Uniconazole promoted conversion of cotton callus to embryogenic callus.
- Taxon
- Cotton (Gossypium hirsutum L.)Gossypium hirsutumt0
- Treatment
- Uniconazolee1
- Outcome
- conversion of callus into an embryogenic callus (EC)e0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promoted the conversion of callus into an embryogenic callus (EC) in cotton.”
e1“Uniconazole, a plant growth regulator”
t0“Cotton (Gossypium hirsutum L.)”
Claim · model-written summary
Uniconazole suppressed IAA level during callus initiation.
- Taxon
- Cotton (Gossypium hirsutum L.)Gossypium hirsutumt0
- Organ / stage
- callus initiatione0
- Treatment
- uniconazolee0
- Outcome
- endogenous auxin [indole acetic acid (IAA)] levele0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The supplementation of uniconazole in the culture medium significantly suppressed the endogenous auxin [indole acetic acid (IAA)] level in callus tissues in both the callus initiation and proliferation stage”
t0“Cotton (Gossypium hirsutum L.)”
Claim · model-written summary
Uniconazole suppressed IAA level during callus proliferation.
- Taxon
- Cotton (Gossypium hirsutum L.)Gossypium hirsutumt0
- Organ / stage
- proliferation stagee0
- Treatment
- uniconazolee0
- Outcome
- endogenous auxin [indole acetic acid (IAA)] levele0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The supplementation of uniconazole in the culture medium significantly suppressed the endogenous auxin [indole acetic acid (IAA)] level in callus tissues in both the callus initiation and proliferation stage”
t0“Cotton (Gossypium hirsutum L.)”
Claim · model-written summary
Uniconazole enhanced ABA level only at the callus proliferation stage.
- Taxon
- Cotton (Gossypium hirsutum L.)Gossypium hirsutumt0
- Organ / stage
- callus proliferation stagee0
- Treatment
- uniconazolee1
- Outcome
- abscisic acid (ABA) levele0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhanced the abscisic acid (ABA) level only in the callus proliferation stage”
e1“uniconazole in the culture medium”
t0“Cotton (Gossypium hirsutum L.)”
Claim · model-written summary
Exogenous ABA and uniconazole cooperatively promoted differentiation of callus to EC.
- Taxon
- Cotton (Gossypium hirsutum L.)Gossypium hirsutumt0
- Treatment
- Exogenous ABA and uniconazolee0
- Outcome
- differentiation rate of callus into ECe0
- Direction
- promotinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous ABA and uniconazole showed cooperative effects on promoting the differentiation rate of callus into EC.”
t0“Cotton (Gossypium hirsutum L.)”
Claim · model-written summary
Uniconazole regulated genes in IAA biosynthesis, metabolism and signaling and ABA metabolism pathways.
- Taxon
- Cotton (Gossypium hirsutum L.)Gossypium hirsutumt0
- Treatment
- uniconazolee0
- Outcome
- genes involved in the IAA biosynthesis, metabolism, and signaling, and ABA metabolism pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the genes involved in the IAA biosynthesis, metabolism, and signaling, and ABA metabolism pathways were regulated by uniconazole during the callus development and SE.”
t0“Cotton (Gossypium hirsutum L.)”
The same questions in other papers
(+)-abscisic acid · growth · Gossypium hirsutum (cotton)Positive and negative results
5 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.