Abstract
Antennaria dioica ( Asteraceae family) – is a perennial herb, commonly found in dry grasslands and sandy or stony places from Eurasian areas. It is known in traditional medicine as antioxidant, diuretic, choleretic and anti-inflammatory remedy. This species should be reconsidered as possible sources of phenols, mainly flavonoids and hydroxycinnamic acids. Thus, the aim of this study was to validate a chromatographic method for detection of phenols and their identification in A. dioica herb. HPLC-DAD method was evaluated in terms of linearity, precision, repeatability, accuracy, LOD and LOQ. The calibration curves of thirteen reference substances were linear (R 2 > 0.99) over the range of 5–400 µg/mL, the LODs and the LOQs were in the range of 0.1–0.3 µg/mL and 0.2–1.0 µg/mL, respectively. During HPLC-DAD assay two flavones – luteolin, apigenin; flavonol – quercetin and three its glycosides – rutin, hyperoside and isoquercitrin; coumarins: coumarin and umbelliferone; five hydroxycinnamic acids – chlorogenic, caffeic, p -coumaric, trans -ferulic and rosmarinic were identified in A. dioica herb. This phytochemical study of A. dioica confirms that this plant material is a rich source of phenolic compounds.
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Claims 10
Claim · model-written summary
Calibration curves for thirteen reference substances were linear over 5–400 µg/mL.
- Taxon
- Antennaria dioica
Antennaria dioicat0 - Outcome
- calibration curvese0
- Value
- R 2 > 0.99e0
- Qualifier
- 5–400 µg/mLe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The calibration curves of thirteen reference substances were linear (R 2 > 0.99) over the range of 5–400 µg/mL”
t0“Antennaria dioica (L.) Gaertn”
Claim · model-written summary
The assay detection limits ranged from 0.1 to 0.3 µg/mL.
- Taxon
- Antennaria dioicaAntennaria dioicat0
- Outcome
- LODse0
- Value
- 0.1–0.3 µg/mLe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the LODs and the LOQs were in the range of 0.1–0.3 µg/mL and 0.2–1.0 µg/mL, respectively”
t0“Antennaria dioica (L.) Gaertn”
Claim · model-written summary
The assay quantification limits ranged from 0.2 to 1.0 µg/mL.
- Taxon
- Antennaria dioicaAntennaria dioicat0
- Outcome
- LOQse0
- Value
- 0.2–1.0 µg/mLe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the LODs and the LOQs were in the range of 0.1–0.3 µg/mL and 0.2–1.0 µg/mL, respectively”
t0“Antennaria dioica (L.) Gaertn”
Claim · model-written summary
Luteolin and apigenin were identified in the herb.
- Taxon
- A. dioicaAntennaria dioicae1
- Organ / stage
- herbe1
- Outcome
- luteolin, apigenine0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two flavones – luteolin, apigenin”
e1“identified in A. dioica herb”
Claim · model-written summary
Quercetin, rutin and hyperoside were identified in the herb.
- Taxon
- A. dioicaAntennaria dioicae1
- Organ / stage
- herbe1
- Outcome
- quercetin and three its glycosides – rutin, hyperosidee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“flavonol – quercetin and three its glycosides – rutin, hyperoside and isoquercitrin”
e1“identified in A. dioica herb”
Claim · model-written summary
Isoquercitrin was identified in the herb.
- Taxon
- A. dioicaAntennaria dioicae1
- Organ / stage
- herbe1
- Outcome
- isoquercitrine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rutin, hyperoside and isoquercitrin”
e1“identified in A. dioica herb”
Claim · model-written summary
Coumarin and umbelliferone were identified in the herb.
- Taxon
- A. dioicaAntennaria dioicae1
- Organ / stage
- herbe1
- Outcome
- coumarin and umbelliferonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“coumarins: coumarin and umbelliferone”
e1“identified in A. dioica herb”
Claim · model-written summary
Chlorogenic, caffeic and p-coumaric acids were identified in the herb.
- Taxon
- A. dioicaAntennaria dioicae1
- Organ / stage
- herbe1
- Outcome
- chlorogenic, caffeic, p -coumarice0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“five hydroxycinnamic acids – chlorogenic, caffeic, p -coumaric, trans -ferulic and rosmarinic”
e1“identified in A. dioica herb”
Claim · model-written summary
Trans-ferulic and rosmarinic acids were identified in the herb.
- Taxon
- A. dioicaAntennaria dioicae1
- Organ / stage
- herbe1
- Outcome
- trans -ferulic and rosmarinice0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“trans -ferulic and rosmarinic”
e1“identified in A. dioica herb”
Claim · model-written summary
The herb was characterized as a rich source of phenolic compounds.
- Taxon
- A. dioicaAntennaria dioicae0
- Outcome
- rich source of phenolic compoundse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A. dioica confirms that this plant material is a rich source of phenolic compounds”