Abstract
In a screen for recessive mutations in Arabidopsis that cause hypersensitivity to abscisic acid (ABA) in germination assays, Hugovieux et al. identified a gene (ABH1) encoding a protein with sequence similarity to messenger RNA cap binding proteins (CBP). In yeast and humans, CBPs are a heterodimeric complex of CBP80 and CBP20. ABH1 interacted with a second Arabidopsis protein called AtCBP20, indicating that the plant CBPs have structural similarity to yeast and human CBPs. Cap-binding activity was measured in a gel-shift assay and depended on expression of both AtCBP20 and ABH1. The abh1 mutants were not significantly altered for auxin, ethylene, brassinosteroid, or jasmonate signaling. DNA array analysis demonstrated that in abh1 mutants, the expression of only a small subset of genes is affected, including a negative regulator of ABA signaling, AtPP2C. Several of the known genes that are down-regulated in abh1 are implicated in signaling pathways for stress and oxidative responses, consistent with known pathways involving ABA. Finally, analysis of guard cell responsiveness to drought and ABA showed that the abh1 mutants were more drought tolerant and exhibited increased calcium transients in response to ABA, consistent with increased sensitivity to ABA. The exact mechanism by which ABH1 regulates ABA signaling remains to be determined, but a CBP that selectively controls the expression of a subset of genes represents a potentially new mechanism for posttranscriptional regulation.V, Hugovieux, J. M. Kwak, J. I. Schroeder, An mRNA cap binding protein, ABH1, modulates early abscisic acid signal transduction in Arabidopsis. Cell 106, 477-487 (2001). [Online Journal]
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Claims 8
Claim · model-written summary
Hugovieux and colleagues identified ABH1 in an Arabidopsis mutation screen.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- ABH1e0
- Outcome
- sequence similarity to messenger RNA cap binding proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In a screen for recessive mutations in Arabidopsis that cause hypersensitivity to abscisic acid (ABA) in germination assays, Hugovieux et al. identified a gene (ABH1) encoding a protein with sequence similarity to messenger RNA cap binding proteins (CBP).”
Claim · model-written summary
ABH1 interacted with AtCBP20.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- ABH1e0
- Outcome
- interactede0
- Qualifier
- AtCBP20e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABH1 interacted with a second Arabidopsis protein called AtCBP20”
Claim · model-written summary
Cap binding required expression of AtCBP20 and ABH1.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- AtCBP20 and ABH1e0
- Outcome
- Cap-binding activitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cap-binding activity was measured in a gel-shift assay and depended on expression of both AtCBP20 and ABH1.”
t0“recessive mutations in Arabidopsis”
Claim · model-written summary
The abh1 mutants showed no significant alteration in four hormone signaling pathways.
- Taxon
- ArabidopsisArabidopsist0
- Material
- abh1 mutantse0
- Outcome
- auxin, ethylene, brassinosteroid, or jasmonate signalinge0
- Direction
- not significantly alterede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The abh1 mutants were not significantly altered for auxin, ethylene, brassinosteroid, or jasmonate signaling.”
t0“recessive mutations in Arabidopsis”
Claim · model-written summary
Only a small subset of genes changed expression in abh1 mutants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- abh1 mutantse0
- Gene
- AtPP2Ce0
- Outcome
- expression of only a small subset of genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in abh1 mutants, the expression of only a small subset of genes is affected, including a negative regulator of ABA signaling, AtPP2C.”
t0“recessive mutations in Arabidopsis”
Claim · model-written summary
Known stress and oxidative response genes were downregulated in abh1.
- Taxon
- ArabidopsisArabidopsist0
- Material
- abh1e0
- Outcome
- known genes that are down-regulatede0
- Direction
- down-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Several of the known genes that are down-regulated in abh1 are implicated in signaling pathways for stress and oxidative responses”
t0“recessive mutations in Arabidopsis”
Claim · model-written summary
The abh1 mutants were more drought tolerant.
- Taxon
- ArabidopsisArabidopsist0
- Material
- abh1 mutantse0
- Outcome
- drought tolerante0
- Direction
- moree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the abh1 mutants were more drought tolerant and exhibited increased calcium transients in response to ABA”
t0“recessive mutations in Arabidopsis”
Claim · model-written summary
The abh1 mutants showed increased calcium transients in response to ABA.
- Taxon
- ArabidopsisArabidopsist0
- Material
- abh1 mutantse0
- Treatment
- ABAe0
- Outcome
- calcium transientse0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the abh1 mutants were more drought tolerant and exhibited increased calcium transients in response to ABA”
t0“recessive mutations in Arabidopsis”