Abstract
Partially purified preparations of lipoxygenase from the germinating barley embryos converted linoleic acid to 9- and 13-hydroperoxy linoleic acids in the ratio of approximately 3:1, while the similar preparations from the ungerminated embryos converted linoleic acid mainly to 9-hydroperoxy linoleic acid. Isoelectric focusing of the partially purified preparations of the germinating embryos revealed the presence of the two lipoxygenase active peaks, having isoelectric point at pH 4.9 and 6.6, respectively. The former peak (barley lipoxygenase-1) was identical to lipoxygenase of the ungerminated embryos, but the latter peak (barley lipoxygenase-2) was found only in the germinating embryos. The newly found isoenzyme, barley lipoxygenase-2, converted linoleic acid mainly to 13-hydroperoxy linoleic acid, and could oxidize esterified derivatives of linoleic acid (methyl linoleate and trilinolein) much strongly than barley lipoxygenase-1.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Germinating barley embryo preparations produced 9- and 13-hydroperoxy linoleic acids at approximately 3:1.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Organ / stage
- germinating barley embryose0
- Gene
- lipoxygenasee0
- Outcome
- 9- and 13-hydroperoxy linoleic acidse0
- Value
- approximately 3:1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“lipoxygenase from the germinating barley embryos converted linoleic acid to 9- and 13-hydroperoxy linoleic acids in the ratio of approximately 3:1”
Claim · model-written summary
Ungerminated embryo preparations converted linoleic acid mainly to 9-hydroperoxy linoleic acid.
- Taxon
- BarleyHordeum vulgaret0
- Organ / stage
- ungerminated embryose0
- Outcome
- 9-hydroperoxy linoleic acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ungerminated embryos converted linoleic acid mainly to 9-hydroperoxy linoleic acid”
t0“Barley”
Claim · model-written summary
Germinating embryo lipoxygenase had two active peaks at pH 4.9 and 6.6.
- Taxon
- BarleyHordeum vulgaret0
- Organ / stage
- germinating embryose0
- Gene
- lipoxygenasee0
- Outcome
- two lipoxygenase active peakse0
- Value
- pH 4.9 and 6.6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“germinating embryos revealed the presence of the two lipoxygenase active peaks, having isoelectric point at pH 4.9 and 6.6, respectively”
t0“Barley”
Claim · model-written summary
Barley lipoxygenase-1 was identical to the enzyme in ungerminated embryos.
- Taxon
- barleyHordeum vulgaree0
- Organ / stage
- ungerminated embryose0
- Gene
- barley lipoxygenase-1e0
- Outcome
- identical to lipoxygenase of the ungerminated embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“barley lipoxygenase-1) was identical to lipoxygenase of the ungerminated embryos”
Claim · model-written summary
Barley lipoxygenase-2 was found only in germinating embryos.
- Taxon
- barleyHordeum vulgaree0
- Organ / stage
- germinating embryose0
- Gene
- barley lipoxygenase-2e0
- Outcome
- found only in the germinating embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“barley lipoxygenase-2) was found only in the germinating embryos”
Claim · model-written summary
Barley lipoxygenase-2 converted linoleic acid mainly to the 13-hydroperoxy product.
- Taxon
- barleyHordeum vulgaree0
- Gene
- barley lipoxygenase-2e0
- Outcome
- 13-hydroperoxy linoleic acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“barley lipoxygenase-2, converted linoleic acid mainly to 13-hydroperoxy linoleic acid”
Claim · model-written summary
Barley lipoxygenase-2 oxidized methyl linoleate and trilinolein more strongly than lipoxygenase-1.
- Taxon
- barleyHordeum vulgaree1
- Gene
- barley lipoxygenase-2e1
- Outcome
- oxidize esterified derivatives of linoleic acid (methyl linoleate and trilinolein)e0
- Direction
- much stronglye0
- Comparator
- barley lipoxygenase-1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“could oxidize esterified derivatives of linoleic acid (methyl linoleate and trilinolein) much strongly than barley lipoxygenase-1”
e1“isoenzyme, barley lipoxygenase-2”