Abstract
Genes encoding nodulation and nitrogen fixation are grouped on the symbiotic plasmid (pSym) in a region that contains genes necessary for betaine catabolism. We used insertional mutagenesis to map genes involved in the demethylation of stachydrine (proline betaine) to a 3.5 kb region to the right of nodD2, a nodulation regulatory gene. Two insertions into this region (stc) created transcriptional fusions that were induced by stachydrine. In both of these, the lacZ reporter was oriented opposite nod box 6, which lies between nodD2 and the stc region, indicating that induction is not dependent of this nod box. All of the Stc− insertional mutants exhibited delayed and reduced nodulation. We take this as evidence for a functional role of stc genes in nodulation.
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Claims 4
Claim · model-written summary
Genes involved in stachydrine demethylation were mapped to a 3.5 kb region to the right of nodD2 in Rhizobium meliloti.
- Taxon
- Rhizobium meliloti
Sinorhizobium melilotit0 - Gene
- nodD2e1
- Outcome
- genes involved in the demethylation of stachydrinee1
- Qualifier
- 3.5 kb region to the right of nodD2e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“required for efficient nodulation by Rhizobium meliloti”
e1“We used insertional mutagenesis to map genes involved in the demethylation of stachydrine (proline betaine) to a 3.5 kb region to the right of nodD2”
Claim · model-written summary
Two insertions in the stc region created transcriptional fusions induced by stachydrine in Rhizobium meliloti.
- Taxon
- Rhizobium melilotiSinorhizobium melilotit0
- Treatment
- stachydrinee2
- Outcome
- transcriptional fusionse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“required for efficient nodulation by Rhizobium meliloti”
e2“Two insertions into this region (stc) created transcriptional fusions that were induced by stachydrine”
Claim · model-written summary
Stc− insertional mutants of Rhizobium meliloti showed reduced nodulation, with delayed nodulation.
- Taxon
- Rhizobium melilotiSinorhizobium melilotit0
- Treatment
- Stc− insertional mutantse3
- Outcome
- nodulatione3
- Direction
- reducede3
- Qualifier
- delayede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“required for efficient nodulation by Rhizobium meliloti”
e3“All of the Stc− insertional mutants exhibited delayed and reduced nodulation”
Claim · model-written summary
The authors interpret the stc genes as having a functional role in nodulation by Rhizobium meliloti.
- Taxon
- Rhizobium melilotiSinorhizobium melilotit0
- Gene
- stc genese4
- Outcome
- nodulatione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“required for efficient nodulation by Rhizobium meliloti”
e4“We take this as evidence for a functional role of stc genes in nodulation”