Abstract
Marker-free transgenic cucumber (Cucumis sativus L.) cv. Poinsett 76 SR plants were produced by Agrobacterium mediated transformation. A transformation efficiency of 1.62 was observed on using Agrobacterium tumefaciens strain LBA4404 harbouring Arabidopsis cbf1 gene driven by the inducible promoter RD29A in a binary vector system pCAMBIA. Transgene integration and single copy insert in transgenic cucumber was confirmed by polymerase chain reaction (PCR) and Southern blot analysis in T0 lines and also confirmed marker-free status in T1 generation. Transgene expression was confirmed by reverse transcription (RT)-PCR in T1 generation transgenic cucumber and advanced to T2 generation. Upon exposure to chilling stress (4 °C), the T2 generation transgenic plants survived up to 36 h; however, wild-type plants could not survive and gradually died. A significant decrease in membrane injury index (MII), increase in activities of antioxidant enzymes (SOD and CAT), free proline content and relative water content (RWC) in the leaves were observed in transgenic cucumber as compared to wild-type under chilling stress. Thus, the transgenic cucumber plants expressing Arabidopsis cbf1 gene conferred protection against chilling stress.
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Claims 8
Claim · model-written summary
Transformation efficiency was 1.62 using strain LBA4404.
- Taxon
- cucumber
Cucumis sativust0 - Gene
- Arabidopsis cbf1 genee0
- Outcome
- transformation efficiencye0
- Value
- 1.62e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A transformation efficiency of 1.62 was observed on using Agrobacterium tumefaciens strain LBA4404 harbouring Arabidopsis cbf1 gene”
t0“Marker-free transgenic cucumber expressing Arabidopsis CBF1 gene confers chilling stress tolerance”
Claim · model-written summary
T0 cucumber lines had integrated single-copy transgenes and T1 plants were marker-free.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- T0 linese0
- Outcome
- Transgene integration and single copy inserte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgene integration and single copy insert in transgenic cucumber was confirmed by polymerase chain reaction (PCR) and Southern blot analysis in T0 lines and also confirmed marker-free status in T1 generation”
Claim · model-written summary
Transgene expression was confirmed in T1 cucumber plants.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- T1 generatione0
- Outcome
- Transgene expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgene expression was confirmed by reverse transcription (RT)-PCR in T1 generation transgenic cucumber”
Claim · model-written summary
T2 transgenic cucumber survived chilling for up to 36 hours whereas wild type died.
- Taxon
- cucumberCucumis sativust0
- Treatment
- chilling stress (4 °C)e0
- Outcome
- transgenic plants survivede0
- Value
- 36 he0
- Comparator
- wild-type plantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Upon exposure to chilling stress (4 °C), the T2 generation transgenic plants survived up to 36 h; however, wild-type plants could not survive and gradually died.”
t0“Marker-free transgenic cucumber expressing Arabidopsis CBF1 gene confers chilling stress tolerance”
Claim · model-written summary
Transgenic cucumber had a lower membrane injury index under chilling than wild type.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- transgenic cucumbere0
- Outcome
- membrane injury index (MII)e0
- Direction
- decreasee0
- Qualifier
- under chilling stresse0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A significant decrease in membrane injury index (MII), increase in activities of antioxidant enzymes (SOD and CAT), free proline content and relative water content (RWC) in the leaves were observed in transgenic cucumber as compared to wild-type under chilling stress.”
Claim · model-written summary
Transgenic cucumber had higher SOD and CAT activities under chilling than wild type.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- transgenic cucumbere0
- Outcome
- activities of antioxidant enzymes (SOD and CAT)e0
- Direction
- increasee0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A significant decrease in membrane injury index (MII), increase in activities of antioxidant enzymes (SOD and CAT), free proline content and relative water content (RWC) in the leaves were observed in transgenic cucumber as compared to wild-type under chilling stress.”
Claim · model-written summary
Transgenic cucumber had more free proline under chilling than wild type.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- transgenic cucumbere0
- Outcome
- free proline contente0
- Direction
- increasee0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A significant decrease in membrane injury index (MII), increase in activities of antioxidant enzymes (SOD and CAT), free proline content and relative water content (RWC) in the leaves were observed in transgenic cucumber as compared to wild-type under chilling stress.”
Claim · model-written summary
Transgenic cucumber had greater leaf relative water content under chilling than wild type.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- leavese0
- Treatment
- transgenic cucumbere0
- Outcome
- relative water content (RWC)e0
- Direction
- increasee0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A significant decrease in membrane injury index (MII), increase in activities of antioxidant enzymes (SOD and CAT), free proline content and relative water content (RWC) in the leaves were observed in transgenic cucumber as compared to wild-type under chilling stress.”