Abstract
Polyethylene glycol (PEG-6000) mediated drought stress was examined in vitro culture under triplicate completely randomized deign. Three drought treatments i.e. T 1 = Zero (Control), T 2 = -0.35 MPa and T 3 = -1.33 MPa were developed. Seeds of the accession were grown on half strength MS media and three explants (leaf, stem, coleoptiles) were used for the callus culture. Stress was applied at the time of the media preparation for callus. Fifty petri plates of each accession were prepared and divided into two groups (Group I, Group II) containing 25 plated each. Petri plates of Group I were maintained for callus culture while of Group II were used for regeneration. Data were recorded from these groups on the traits i.e. relative fresh weight (g), proline content (g), relative membrane leakage (g), total soluble sugar contents (g), coleoptiles length (mm), root length (mm), root count, fresh and dry root weight (g) and fresh and dry shoot weight (g). On the basis of the above parameters, the accessions A-23 and HBRS-1 were drought tolerant and 017577 as drought sensitive. In addition, the results of this study illustrate the potential of using in vitro culture for evaluation of drought tolerance at cellular level.
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Claims 4
Claim · model-written summary
Sunflower accession A-23 was drought tolerant under PEG-mediated in vitro drought stress.
- Taxon
- Sunflower
Helianthus annuust0 - Material
- A-23e0
- Treatment
- Polyethylene glycol (PEG-6000) mediated drought stresse1
- Outcome
- drought tolerante0
- Setting
- in vitro culturee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sunflower (Helianthus Annuus L.) Accessions”
e0“the accessions A-23 and HBRS-1 were drought tolerant and 017577 as drought sensitive”
e1“Polyethylene glycol (PEG-6000) mediated drought stress was examined in vitro culture”
Claim · model-written summary
Sunflower accession HBRS-1 was drought tolerant under PEG-mediated in vitro drought stress.
- Taxon
- SunflowerHelianthus annuust0
- Material
- HBRS-1e0
- Treatment
- Polyethylene glycol (PEG-6000) mediated drought stresse1
- Outcome
- drought tolerante0
- Setting
- in vitro culturee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sunflower (Helianthus Annuus L.) Accessions”
e0“the accessions A-23 and HBRS-1 were drought tolerant and 017577 as drought sensitive”
e1“Polyethylene glycol (PEG-6000) mediated drought stress was examined in vitro culture”
Claim · model-written summary
Sunflower accession 017577 was drought sensitive under PEG-mediated in vitro drought stress.
- Taxon
- SunflowerHelianthus annuust0
- Material
- 017577e0
- Treatment
- Polyethylene glycol (PEG-6000) mediated drought stresse1
- Outcome
- drought sensitivee0
- Setting
- in vitro culturee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sunflower (Helianthus Annuus L.) Accessions”
e0“the accessions A-23 and HBRS-1 were drought tolerant and 017577 as drought sensitive”
e1“Polyethylene glycol (PEG-6000) mediated drought stress was examined in vitro culture”
Claim · model-written summary
The authors conclude that in vitro culture has potential for evaluating drought tolerance at the cellular level.
- Taxon
- SunflowerHelianthus annuust0
- Outcome
- evaluation of drought tolerance at cellular levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sunflower (Helianthus Annuus L.) Accessions”
e0“the results of this study illustrate the potential of using in vitro culture for evaluation of drought tolerance at cellular level”