Paper · 2019 · International Journal of Biosciences (IJB)

A comparative study of total phenolic contents and antioxidant potential of seeds of Peganum harmala

Zafar Iqbal; Mohsin Javed; Ghazala Rafique; et al.

12
claims
28
verbatim quotes
2
citations
1
research areas
✓
Open access

Abstract

Various solvents including methanol, hexane, benzene, chloroform and dichloromethane were utilized for the extraction of phenolic contents from the seeds of pegnum harmala. Total phenolic contents (TPC) were determined by using Folin-Ciocalteu reagent method against gallic acid as standard by UV-Vis spectrophotometer at 765 nm while DPPH free radical scavenging activity of the extracts was measure by using UV-Vis spectrophotometer and taking reading at 517 nm and ascorbic acid as standard. Total phenolic contents 27.7 mg GA/g, 22.2 mg GA/g, 26.4 mg GA /g, 30.7 mg GA /g and 17.3 mg GA /g were for dichloromethane, benzene, chloroform, methanol and hexane extracts respectively. Methanol extract showed high TPC content and high antioxidant activity (72%) followed by dichloromethane extract (67%), chloroform extract (63 %), benzene extract (52 %) and hexane extract (48 %) respectively for 50 L of each sample. The antioxidant activity was concentrated dependent for all the solvents and high TPC contents showed higher antioxidant activity.

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Claims 12

Observationobservedoriginal resultmeasured

Claim · model-written summary

Dichloromethane seed extract had total phenolics of 27.7 mg GA/g.

Taxon
Peganum harmalaPeganum harmalat0
Treatment
dichloromethanee0
Outcome
Total phenolic contentse0
Value
27.7 mg GA/ge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Peganum harmala
Peganum harmala · TaxonYuriy75 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Total phenolic contents 27.7 mg GA/g, 22.2 mg GA/g, 26.4 mg GA /g, 30.7 mg GA /g and 17.3 mg GA /g were for dichloromethane, benzene, chloroform, methanol and hexane extracts respectively”

✓ located verbatim in the source · abstract, characters 495–682 · show in text ↑
t0

“Peganum harmala”

✓ located verbatim in the source · title, characters 85–100 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Benzene seed extract had total phenolics of 22.2 mg GA/g.

Taxon
Peganum harmalaPeganum harmalat0
Treatment
benzenee0
Outcome
Total phenolic contentse0
Value
22.2 mg GA/ge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Peganum harmala
Peganum harmala · TaxonYuriy75 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Total phenolic contents 27.7 mg GA/g, 22.2 mg GA/g, 26.4 mg GA /g, 30.7 mg GA /g and 17.3 mg GA /g were for dichloromethane, benzene, chloroform, methanol and hexane extracts respectively”

✓ located verbatim in the source · abstract, characters 495–682 · show in text ↑
t0

“Peganum harmala”

✓ located verbatim in the source · title, characters 85–100 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Chloroform seed extract had total phenolics of 26.4 mg GA /g.

Taxon
Peganum harmalaPeganum harmalat0
Treatment
chloroforme0
Outcome
Total phenolic contentse0
Value
26.4 mg GA /ge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Peganum harmala
Peganum harmala · TaxonYuriy75 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Total phenolic contents 27.7 mg GA/g, 22.2 mg GA/g, 26.4 mg GA /g, 30.7 mg GA /g and 17.3 mg GA /g were for dichloromethane, benzene, chloroform, methanol and hexane extracts respectively”

✓ located verbatim in the source · abstract, characters 495–682 · show in text ↑
t0

“Peganum harmala”

✓ located verbatim in the source · title, characters 85–100 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Methanol seed extract had total phenolics of 30.7 mg GA /g, the highest among solvents.

Taxon
Peganum harmalaPeganum harmalat0
Treatment
methanole0
Outcome
Total phenolic contentse0
Value
30.7 mg GA /ge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Peganum harmala
Peganum harmala · TaxonYuriy75 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Total phenolic contents 27.7 mg GA/g, 22.2 mg GA/g, 26.4 mg GA /g, 30.7 mg GA /g and 17.3 mg GA /g were for dichloromethane, benzene, chloroform, methanol and hexane extracts respectively”

✓ located verbatim in the source · abstract, characters 495–682 · show in text ↑
t0

“Peganum harmala”

✓ located verbatim in the source · title, characters 85–100 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Hexane seed extract had total phenolics of 17.3 mg GA /g.

Taxon
Peganum harmalaPeganum harmalat0
Treatment
hexanee0
Outcome
Total phenolic contentse0
Value
17.3 mg GA /ge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Peganum harmala
Peganum harmala · TaxonYuriy75 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Total phenolic contents 27.7 mg GA/g, 22.2 mg GA/g, 26.4 mg GA /g, 30.7 mg GA /g and 17.3 mg GA /g were for dichloromethane, benzene, chloroform, methanol and hexane extracts respectively”

✓ located verbatim in the source · abstract, characters 495–682 · show in text ↑
t0

“Peganum harmala”

✓ located verbatim in the source · title, characters 85–100 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Methanol extract showed 72% antioxidant activity.

Taxon
Peganum harmalaPeganum harmalat0
Treatment
Methanole0
Outcome
antioxidant activitye0
Value
72%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Peganum harmala
Peganum harmala · TaxonYuriy75 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Methanol extract showed high TPC content and high antioxidant activity (72%)”

✓ located verbatim in the source · abstract, characters 684–760 · show in text ↑
t0

“Peganum harmala”

✓ located verbatim in the source · title, characters 85–100 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Dichloromethane extract showed 67% antioxidant activity.

Taxon
Peganum harmalaPeganum harmalat0
Treatment
dichloromethanee0
Outcome
antioxidant activityt1
Value
67%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Peganum harmala
Peganum harmala · TaxonYuriy75 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“followed by dichloromethane extract (67%), chloroform extract (63 %), benzene extract (52 %) and hexane extract (48 %) respectively for 50 L of each sample”

✓ located verbatim in the source · abstract, characters 761–916 · show in text ↑
t0

“Peganum harmala”

✓ located verbatim in the source · title, characters 85–100 · show in text ↑
t1

“high antioxidant activity (72%)”

✓ located verbatim in the source · abstract, characters 729–760 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Chloroform extract showed 63% antioxidant activity.

Taxon
Peganum harmalaPeganum harmalat0
Treatment
chloroforme0
Outcome
antioxidant activityt1
Value
63 %e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Peganum harmala
Peganum harmala · TaxonYuriy75 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“followed by dichloromethane extract (67%), chloroform extract (63 %), benzene extract (52 %) and hexane extract (48 %) respectively for 50 L of each sample”

✓ located verbatim in the source · abstract, characters 761–916 · show in text ↑
t0

“Peganum harmala”

✓ located verbatim in the source · title, characters 85–100 · show in text ↑
t1

“high antioxidant activity (72%)”

✓ located verbatim in the source · abstract, characters 729–760 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Benzene extract showed 52% antioxidant activity.

Taxon
Peganum harmalaPeganum harmalat0
Treatment
benzenee0
Outcome
antioxidant activityt1
Value
52 %e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Peganum harmala
Peganum harmala · TaxonYuriy75 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“followed by dichloromethane extract (67%), chloroform extract (63 %), benzene extract (52 %) and hexane extract (48 %) respectively for 50 L of each sample”

✓ located verbatim in the source · abstract, characters 761–916 · show in text ↑
t0

“Peganum harmala”

✓ located verbatim in the source · title, characters 85–100 · show in text ↑
t1

“high antioxidant activity (72%)”

✓ located verbatim in the source · abstract, characters 729–760 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Hexane extract showed 48% antioxidant activity.

Taxon
Peganum harmalaPeganum harmalat0
Treatment
hexanee0
Outcome
antioxidant activityt1
Value
48 %e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Peganum harmala
Peganum harmala · TaxonYuriy75 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“followed by dichloromethane extract (67%), chloroform extract (63 %), benzene extract (52 %) and hexane extract (48 %) respectively for 50 L of each sample”

✓ located verbatim in the source · abstract, characters 761–916 · show in text ↑
t0

“Peganum harmala”

✓ located verbatim in the source · title, characters 85–100 · show in text ↑
t1

“high antioxidant activity (72%)”

✓ located verbatim in the source · abstract, characters 729–760 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Antioxidant activity depended on extract concentration.

Taxon
Peganum harmalaPeganum harmalat0
Treatment
all the solventse0
Outcome
antioxidant activitye0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Peganum harmala
Peganum harmala · TaxonYuriy75 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The antioxidant activity was concentrated dependent for all the solvents”

✓ located verbatim in the source · abstract, characters 918–990 · show in text ↑
t0

“Peganum harmala”

✓ located verbatim in the source · title, characters 85–100 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Higher total phenolic content was associated with higher antioxidant activity.

Taxon
Peganum harmalaPeganum harmalat0
Outcome
antioxidant activitye0
Direction
highere0
Qualifier
high TPC contentse0

Not reportedCompound

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Peganum harmala
Peganum harmala · TaxonYuriy75 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“high TPC contents showed higher antioxidant activity”

✓ located verbatim in the source · abstract, characters 995–1047 · show in text ↑
t0

“Peganum harmala”

✓ located verbatim in the source · title, characters 85–100 · show in text ↑