Abstract
Leaf analysis is essential for diagnosing nutritional status and guiding fertilizer application. The present study aimed to investigate the appropriate time for leaf sampling and the effect of genotype on olive nutrition. We determined leaf nutrient concentrations of calcium (Ca), iron (Fe), and manganese (Mn) in five Greek (‘Amfissis’, ‘Chondrolia Chalkidikis’, ‘Koroneiki’, ‘Mastoidis’, and ‘Kalamon’) and one Spanish (‘Picual’) variety from May 2019 to April 2020. The concentrations of Ca, Fe, and Mn were significantly affected by genotype and season. The highest concentrations for all nutrients were determined in April, while the lowest were in May, June, and October. Leaf Ca concentration significantly increased progressively from May to September for all the varieties. Leaves of ‘Koroneiki’ had the highest Ca concentration. Iron concentrations were within the sufficiency thresholds for all the varieties during the whole experimental period, and ‘Mastoidis’ showed the highest concentration. Leaf Mn concentration for all the varieties increased from May to September with an instant decrease in June, apart from ‘Amfissis’. The varieties ‘Kalamon’ and ‘Chondrolia Chalkidikis’ were found to be above the Mn sufficiency threshold throughout the year. Variations among season and genotype depict the complexity of nutrient transportation in leaf tissues.
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Claims 10
Claim · model-written summary
Leaf Ca, Fe, and Mn concentrations were significantly affected by genotype and season.
- Taxon
- Olive
Olea europaeat0 - Material
- genotypee0
- Outcome
- concentrations of Ca, Fe, and Mne0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The concentrations of Ca, Fe, and Mn were significantly affected by genotype and season”
t0“Olive”
Claim · model-written summary
All three nutrient concentrations peaked in April.
- Taxon
- Olive
Olea europaeat0 - Outcome
- concentrations for all nutrientse0
- Direction
- higheste0
- Qualifier
- Aprile0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The highest concentrations for all nutrients were determined in April”
t0“Olive”
Claim · model-written summary
All three nutrient concentrations were lowest in May, June, and October.
- Taxon
- Olive
Olea europaeat0 - Outcome
- concentrationse0
- Direction
- loweste0
- Qualifier
- May, June, and Octobere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The highest concentrations for all nutrients were determined in April, while the lowest were in May, June, and October”
t0“Olive”
Claim · model-written summary
Leaf Ca increased significantly from May to September across varieties.
- Taxon
- Olive
Olea europaeat0 - Outcome
- Leaf Ca concentratione0
- Direction
- increasede0
- Qualifier
- May to Septembere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Leaf Ca concentration significantly increased progressively from May to September for all the varieties”
t0“Olive”
Claim · model-written summary
Koroneiki had the highest leaf Ca concentration.
- Taxon
- Olive
Olea europaeat0 - Material
- ‘Koroneiki’e0
- Outcome
- Ca concentratione0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Leaves of ‘Koroneiki’ had the highest Ca concentration”
t0“Olive”
Claim · model-written summary
Iron concentrations remained within sufficiency thresholds for every variety throughout the study.
- Taxon
- Olive
Olea europaeat0 - Outcome
- Iron concentrationse0
- Qualifier
- within the sufficiency thresholdse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Iron concentrations were within the sufficiency thresholds for all the varieties during the whole experimental period”
t0“Olive”
Claim · model-written summary
Mastoidis had the highest iron concentration.
- Taxon
- Olive
Olea europaeat0 - Material
- ‘Mastoidis’e0
- Outcome
- concentratione0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“‘Mastoidis’ showed the highest concentration”
t0“Olive”
Claim · model-written summary
Leaf Mn increased from May to September, with a June decrease, except in Amfissis.
- Taxon
- Olive
Olea europaeat0 - Outcome
- Leaf Mn concentratione0
- Direction
- increasede0
- Qualifier
- apart from ‘Amfissis’e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Leaf Mn concentration for all the varieties increased from May to September with an instant decrease in June, apart from ‘Amfissis’”
t0“Olive”
Claim · model-written summary
Kalamon exceeded the Mn sufficiency threshold throughout the year.
- Taxon
- Olive
Olea europaeat0 - Material
- ‘Kalamon’e0
- Outcome
- Mn sufficiency thresholde0
- Direction
- abovee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The varieties ‘Kalamon’ and ‘Chondrolia Chalkidikis’ were found to be above the Mn sufficiency threshold throughout the year”
t0“Olive”
Claim · model-written summary
Chondrolia Chalkidikis exceeded the Mn sufficiency threshold throughout the year.
- Taxon
- Olive
Olea europaeat0 - Material
- ‘Chondrolia Chalkidikis’e0
- Outcome
- Mn sufficiency thresholde0
- Direction
- abovee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The varieties ‘Kalamon’ and ‘Chondrolia Chalkidikis’ were found to be above the Mn sufficiency threshold throughout the year”
t0“Olive”