Abstract
The effect of Bacillus subtilis bacteria in combination with salicylic (SA) and jasmonic (JA) acids on changes in the proteome of potato leaves during infection with Phytophthora infestans (Mont.) de Bary and moisture deficit was studied. Plants grown from microtubers of the Rannyaya Roza variety were sprayed with a suspension of B. subtilis (108 cells/mL) and a mixture of bacteria with SA (10–6 M), JA (10–7 M), and SA + JA. The plants were infected with P. infestans (105 spores/mL) 3 days after treatment and cultivated under conditions of artificial soil drought by reducing watering. When the soil moisture reached 40 ± 5% of the total water capacity (7 days after infection), the plants were fixed in liquid nitrogen to isolate proteins and analyze them by two-dimensional electrophoresis and mass spectrometry. A decrease in the degree of infestation with P. infestans was shown when potato leaves were treated with B. subtilis in combination with SA and JA. The leaf proteome shows differences in the content of 14 proteins in the pI range from 4.0 to 9.0 with molecular masses from 30 to 125 kDa. The most significant changes in the spectrum of plant proteins were found in healthy plants treated with B. subtilis and in infected plants in the combination of B. subtilis with JA. Qualitative and quantitative changes were observed for proteins involved in the processes of respiration and hypersensitivity reactions (HSR), energy metabolism, synthesis of secondary metabolites, and protective proteins that affect plant resistance to abiotic and biotic stress. Proteomic analysis has identified important proteins involved in the mechanism of potato responses to treatment with B. subtilis and infection with P. infestans, although the exact functions of the identified proteins and their potential impact on potato plant resistance to P. infestans and moisture deficit remain to be elucidated.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 4
Claim · model-written summary
B. subtilis combined with SA and JA reduced P. infestans infestation of potato leaves.
- Taxon
- potato
Solanum tuberosume0 - Treatment
- B. subtilis in combination with SA and JAe0
- Interactor
- P. infestans
Phytophthora infestanse0pathogen - Second interactor
- B. subtilis
Bacillus subtilise0biocontrol_agent - Outcome
- degree of infestation with P. infestanse0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A decrease in the degree of infestation with P. infestans was shown when potato leaves were treated with B. subtilis in combination with SA and JA”
Claim · model-written summary
The leaf proteome differed in content of 14 proteins.
- Taxon
- PotatoSolanum tuberosumt0
- Organ / stage
- Leavest0
- Outcome
- differences in the content of 14 proteinse0
- Value
- 14 proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The leaf proteome shows differences in the content of 14 proteins in the pI range from 4.0 to 9.0 with molecular masses from 30 to 125 kDa”
t0“Potato Leaves”
Claim · model-written summary
The strongest protein spectrum changes occurred in healthy plants treated with B. subtilis.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- B. subtilise0
- Interactor
- B. subtilisBacillus subtilise0biocontrol_agent
- Outcome
- changes in the spectrum of plant proteinse0
- Direction
- most significante0
- Qualifier
- healthy plantse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The most significant changes in the spectrum of plant proteins were found in healthy plants treated with B. subtilis and in infected plants in the combination of B. subtilis with JA”
t0“Potato Leaves”
Claim · model-written summary
The strongest protein spectrum changes also occurred in infected plants given B. subtilis with JA.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- B. subtilis with JAe0
- Interactor
- B. subtilisBacillus subtilise0biocontrol_agent
- Outcome
- changes in the spectrum of plant proteinse0
- Direction
- most significante0
- Qualifier
- infected plantse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The most significant changes in the spectrum of plant proteins were found in healthy plants treated with B. subtilis and in infected plants in the combination of B. subtilis with JA”
t0“Potato Leaves”
The same questions in other papers
salicylic acid · Phytophthora infestans · Solanum tuberosum (potato)Positive and negative results
9 papers in the same direction as this paper · 2 in the opposite direction
- positive
- no effect
- negative
8 papers more in this group
See all 12 papers →jasmonic acid · Phytophthora infestans · Solanum tuberosum (potato)Results agree
8 papers in the same direction as this paper
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Bacillus subtilis · Phytophthora infestans · Solanum tuberosum (potato)Results agree
13 papers in the same direction as this paper
- positive
- positive
- positive
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.