Abstract
This study evaluated the seasonal of two flavonoids, antioxidant activity, chromatographic profiles by Thin Layer Chromatography, and High-Performance Liquid Chromatography, in Erythroxylum suberosum A.St.-Hil. These variables were observed from August 2013 to May 2014 in correlation with climatic variables such as temperature, rainfall index and global radiation. The chromatographic profiles were similar in the aqueous and ethanol extracts, and the flavonoids hyperoside and isoquercitrin were identified and quantified. In the inhibition of the DPPH • radical, the most active was the aqueous extract from the 2 nd collection (IC 50 : 4.45 μg/mL). For the phosphomolybdenum complex reduction method, ethanol extract from the 1 st collection was active (206.39 μg/mL equivalent ascorbic acid). Regarding the environmental correlations, it was observed that the higher global radiation index has a strong influence on the concentrations of hyperoside and contributes to the antioxidant activity. On the other hand, higher temperatures contributed to higher isoquercitrin content in aqueous extracts. Therefore, the results indicated that August is the best collection period for Erythroxylum suberosum A.St.-Hil. leaves, in order to obtain higher isoquercitrin and hyperoside contents and, consequently, higher antioxidant activity.
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Claims 7
Claim · model-written summary
Aqueous and ethanol extracts had similar chromatographic profiles.
- Taxon
- Erythroxylum suberosum A. St.-Hil.
Erythroxylum suberosumt0 - Outcome
- chromatographic profilese0
- Direction
- similare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The chromatographic profiles were similar in the aqueous and ethanol extracts”
t0“Erythroxylum suberosum A. St.-Hil.”
Claim · model-written summary
Hyperoside and isoquercitrin were identified and quantified.
- Taxon
- Erythroxylum suberosum A. St.-Hil.Erythroxylum suberosumt0
- Outcome
- hyperoside and isoquercitrin were identified and quantifiede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the flavonoids hyperoside and isoquercitrin were identified and quantified”
t0“Erythroxylum suberosum A. St.-Hil.”
Claim · model-written summary
The aqueous extract from the second collection had the strongest DPPH radical inhibition.
- Taxon
- Erythroxylum suberosum A. St.-Hil.Erythroxylum suberosumt0
- Material
- aqueous extract from the 2 nd collectione0
- Outcome
- inhibition of the DPPH • radicale0
- Direction
- most activee0
- Value
- 4.45 μg/mLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the inhibition of the DPPH • radical, the most active was the aqueous extract from the 2 nd collection (IC 50 : 4.45 μg/mL)”
t0“Erythroxylum suberosum A. St.-Hil.”
Claim · model-written summary
The ethanol extract from the first collection was active in the phosphomolybdenum assay.
- Taxon
- Erythroxylum suberosum A. St.-Hil.Erythroxylum suberosumt0
- Material
- ethanol extract from the 1 st collectione0
- Outcome
- phosphomolybdenum complex reductione0
- Value
- 206.39 μg/mL equivalent ascorbic acide0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“For the phosphomolybdenum complex reduction method, ethanol extract from the 1 st collection was active (206.39 μg/mL equivalent ascorbic acid)”
t0“Erythroxylum suberosum A. St.-Hil.”
Claim · model-written summary
Higher global radiation was associated with hyperoside concentration and antioxidant activity.
- Taxon
- Erythroxylum suberosum A. St.-Hil.Erythroxylum suberosumt0
- Outcome
- concentrations of hyperoside and contributes to the antioxidant activitye0
- Setting
- global radiation indexe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the higher global radiation index has a strong influence on the concentrations of hyperoside and contributes to the antioxidant activity”
t0“Erythroxylum suberosum A. St.-Hil.”
Claim · model-written summary
Higher temperatures were associated with higher isoquercitrin content in aqueous extracts.
- Taxon
- Erythroxylum suberosum A. St.-Hil.Erythroxylum suberosumt0
- Material
- aqueous extractse0
- Outcome
- isoquercitrin contente0
- Direction
- highere0
- Setting
- higher temperaturese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher temperatures contributed to higher isoquercitrin content in aqueous extracts”
t0“Erythroxylum suberosum A. St.-Hil.”
Claim · model-written summary
The authors identify August as the best leaf collection period for higher flavonoid content and antioxidant activity.
- Taxon
- Erythroxylum suberosum A.St.-Hil.Erythroxylum suberosume0
- Organ / stage
- leavese0
- Outcome
- August is the best collection periode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“August is the best collection period for Erythroxylum suberosum A.St.-Hil. leaves, in order to obtain higher isoquercitrin and hyperoside contents and, consequently, higher antioxidant activity”