Abstract
Herbaspirillum seropedicae is an endophytic diazotrophic bacterium, which associates with important agricultural plants. In the present study, we have investigated the attachment to and internal colonization of Phaseolus vulgaris roots by the H. seropedicae wild-type strain SMR1 and by a strain of H. seropedicae expressing a red fluorescent protein (DsRed) to track the bacterium in the plant tissues. Two-day-old P. vulgaris roots were incubated at 30°C for 15 min with 6 x 10(8) CFU/mL H. seropedicae SMR1 or RAM4. Three days after inoculation, 4 x 10(4) cells of endophytic H. seropedicae SMR1 were recovered per gram of fresh root, and 9 days after inoculation the number of endophytes increased to 4 x 10(6) CFU/g. The identity of the recovered bacteria was confirmed by amplification and sequencing of the 16SrRNA gene. Furthermore, confocal microscopy of P. vulgaris roots inoculated with H. seropedicae RAM4 showed that the bacterial cells were attached to the root surface 15 min after inoculation; fluorescent bacteria were visible in the internal tissues after 24 h and were found in the central cylinder after 72 h, showing that H. seropedicae RAM4 is capable of colonizing the roots of the dicotyledon P. vulgaris. Determination of dry weight of common bean inoculated with H. seropedicae SMR1 suggested that this bacterium has a negative effect on the growth of P. vulgaris.
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Claims 6
Claim · model-written summary
Endophytic H. seropedicae SMR1 recovery was 4 x 10(4) cells per gram of fresh root three days after inoculation.
- Taxon
- Phaseolus vulgaris
Phaseolus vulgarise0 - Organ / stage
- rootse0
- Interactor
- H. seropedicae SMR1Herbaspirillum seropedicaee1symbiont
- Outcome
- cells of endophytic H. seropedicae SMR1 were recovered per gram of fresh roote1
- Value
- 4 x 10(4) cellse1
- Qualifier
- Three days after inoculatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phaseolus vulgaris roots”
e1“Three days after inoculation, 4 x 10(4) cells of endophytic H. seropedicae SMR1 were recovered per gram of fresh root”
Claim · model-written summary
Endophytic H. seropedicae abundance rose to 4 x 10(6) CFU/g nine days after inoculation.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Interactor
- H. seropedicae SMR1Herbaspirillum seropedicaee2symbiont
- Outcome
- number of endophytese1
- Direction
- increasede1
- Value
- 4 x 10(6) CFU/ge1
- Qualifier
- 9 days after inoculatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phaseolus vulgaris roots”
e1“9 days after inoculation the number of endophytes increased to 4 x 10(6) CFU/g”
e2“endophytic H. seropedicae SMR1 were recovered”
Claim · model-written summary
H. seropedicae RAM4 attached to P. vulgaris root surfaces 15 minutes after inoculation.
- Taxon
- P. vulgarisPhaseolus vulgarise0
- Organ / stage
- root surfacee0
- Interactor
- H. seropedicae RAM4Herbaspirillum seropedicaee0symbiont
- Outcome
- bacterial cells were attached to the root surfacee0
- Qualifier
- 15 min after inoculatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P. vulgaris roots inoculated with H. seropedicae RAM4 showed that the bacterial cells were attached to the root surface 15 min after inoculation”
Claim · model-written summary
H. seropedicae RAM4 was visible in internal P. vulgaris root tissues after 24 hours.
- Taxon
- P. vulgarisPhaseolus vulgarise0
- Organ / stage
- internal tissuese1
- Interactor
- H. seropedicae RAM4Herbaspirillum seropedicaee0symbiont
- Outcome
- fluorescent bacteria were visible in the internal tissuese1
- Qualifier
- after 24 he1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P. vulgaris roots inoculated with H. seropedicae RAM4”
e1“fluorescent bacteria were visible in the internal tissues after 24 h”
Claim · model-written summary
H. seropedicae RAM4 reached the central cylinder of P. vulgaris roots after 72 hours.
- Taxon
- P. vulgarisPhaseolus vulgarise0
- Organ / stage
- central cylindere1
- Interactor
- H. seropedicae RAM4Herbaspirillum seropedicaee0symbiont
- Outcome
- found in the central cylindere1
- Qualifier
- after 72 he1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P. vulgaris roots inoculated with H. seropedicae RAM4”
e1“were found in the central cylinder after 72 h”
Claim · model-written summary
H. seropedicae SMR1 may reduce P. vulgaris dry weight.
- Taxon
- P. vulgarisPhaseolus vulgarise0
- Treatment
- inoculated with H. seropedicae SMR1e0
- Interactor
- H. seropedicae SMR1Herbaspirillum seropedicaee0symbiont
- Outcome
- dry weight of common beane0
- Direction
- negative effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dry weight of common bean inoculated with H. seropedicae SMR1 suggested that this bacterium has a negative effect on the growth of P. vulgaris”
The same questions in other papers
Herbaspirillum seropedicae · growth · Phaseolus vulgaris (common bean)Results agree
1 paper in the same direction as this paper
- negative
inoculation · growth · Phaseolus vulgaris (common bean)Results differ
19 papers in the same direction as this paper · 106 in the opposite direction
- positive
- positive
- positive
122 papers more in this group
See all 126 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.