Abstract
Mitogenomes of most flowering plants evolve slowly in sequence, but rapidly in structure. The rearrangements in structure are mainly caused by repeat-mediated recombination. However, patterns of repeat-mediated recombination vary substantially among plants, and to provide a comprehensive picture, characterization of repeat-mediated recombination should extend to more plant species, including parasitic plants with a distinct heterotrophic lifestyle. Here we assembled the mitogenome of the holoparasitic plant Aeginetia indica (Orobanchaceae) using Illumina sequencing reads. The mitogenome was assembled into a circular chromosome of 420,362 bp, 18,734 bp longer than that of another individual of A. indica which was assembled before as a linear molecule. Synteny analysis between the two mitogenomes revealed numerous rearrangements, unique regions of each individual and 0.2% sequence divergence in their syntenic regions. The A. indica mitogenome contains a gene content typical of flowering plants (33 protein-coding, 3 rRNA, and 17 tRNA genes). Repetitive sequences >30 bp in size totals 57,060 bp, representing 13.6% of the mitogenome. We examined recombination mediated by repeats >100 bp in size and found highly active recombination for all the repeats, including a very large repeat of ~16 kb. Recombination between these repeats can form much smaller subgenomic circular chromosomes, which may lead to rapid replication of mitochondrial DNA and thus be advantageous for A. indica with a parasitic lifestyle. In addition, unlike some other parasitic plants, A. indica shows no evidence for horizontal gene transfer of protein-coding genes in its mitogenome.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
The Aeginetia indica mitogenome was assembled as a circular chromosome of 420,362 bp, 18,734 bp longer than a previously assembled individual.
- Taxon
- Aeginetia indica
Aeginetia indicae0 - Outcome
- assembled into a circular chromosomee1
- Value
- 420,362 bpe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we assembled the mitogenome of the holoparasitic plant Aeginetia indica (Orobanchaceae) using Illumina sequencing reads”
e1“The mitogenome was assembled into a circular chromosome of 420,362 bp, 18,734 bp longer than that of another individual of A. indica”
Claim · model-written summary
Synteny analysis between two A. indica mitogenomes revealed numerous rearrangements and unique regions of each individual.
- Taxon
- Aeginetia indicaAeginetia indicae0
- Outcome
- numerous rearrangements, unique regions of each individuale1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we assembled the mitogenome of the holoparasitic plant Aeginetia indica (Orobanchaceae) using Illumina sequencing reads”
e1“Synteny analysis between the two mitogenomes revealed numerous rearrangements, unique regions of each individual”
Claim · model-written summary
Syntenic regions of the two A. indica mitogenomes show 0.2% sequence divergence.
- Taxon
- Aeginetia indicaAeginetia indicae0
- Outcome
- sequence divergence in their syntenic regionse1
- Value
- 0.2%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we assembled the mitogenome of the holoparasitic plant Aeginetia indica (Orobanchaceae) using Illumina sequencing reads”
e1“Synteny analysis between the two mitogenomes revealed numerous rearrangements, unique regions of each individual and 0.2% sequence divergence in their syntenic regions”
Claim · model-written summary
The A. indica mitogenome has a gene content typical of flowering plants: 33 protein-coding, 3 rRNA and 17 tRNA genes.
- Taxon
- A. indicaAeginetia indicae0
- Outcome
- gene contente0
- Value
- 33 protein-coding, 3 rRNA, and 17 tRNA genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The A. indica mitogenome contains a gene content typical of flowering plants (33 protein-coding, 3 rRNA, and 17 tRNA genes)”
Claim · model-written summary
Repetitive sequences longer than 30 bp total 57,060 bp, representing 13.6% of the mitogenome.
- Taxon
- Aeginetia indicaAeginetia indicae0
- Outcome
- Repetitive sequences >30 bp in sizee1
- Value
- 57,060 bpe1
- Qualifier
- representing 13.6% of the mitogenomee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we assembled the mitogenome of the holoparasitic plant Aeginetia indica (Orobanchaceae) using Illumina sequencing reads”
e1“Repetitive sequences >30 bp in size totals 57,060 bp, representing 13.6% of the mitogenome”
Claim · model-written summary
Recombination mediated by repeats longer than 100 bp is highly active for all repeats, including a repeat of about 16 kb.
- Taxon
- Aeginetia indicaAeginetia indicae0
- Outcome
- highly active recombinatione1
- Qualifier
- a very large repeat of ~16 kbe1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we assembled the mitogenome of the holoparasitic plant Aeginetia indica (Orobanchaceae) using Illumina sequencing reads”
e1“We examined recombination mediated by repeats >100 bp in size and found highly active recombination for all the repeats, including a very large repeat of ~16 kb”
Claim · model-written summary
Recombination between repeats may form smaller subgenomic circular chromosomes, which may lead to rapid mitochondrial DNA replication.
- Taxon
- Aeginetia indicaAeginetia indicae0
- Outcome
- rapid replication of mitochondrial DNAe1
- Qualifier
- much smaller subgenomic circular chromosomese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we assembled the mitogenome of the holoparasitic plant Aeginetia indica (Orobanchaceae) using Illumina sequencing reads”
e1“Recombination between these repeats can form much smaller subgenomic circular chromosomes, which may lead to rapid replication of mitochondrial DNA”
Claim · model-written summary
The A. indica mitogenome shows no evidence for horizontal gene transfer of protein-coding genes.
- Taxon
- Aeginetia indicaAeginetia indicae0
- Outcome
- evidence for horizontal gene transfer of protein-coding genese1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we assembled the mitogenome of the holoparasitic plant Aeginetia indica (Orobanchaceae) using Illumina sequencing reads”
e1“A. indica shows no evidence for horizontal gene transfer of protein-coding genes in its mitogenome”