Abstract
Ustilago esculenta is a fungus of two morphological forms, among the filamentous dikaryon that can induce the plant stem to expand to form fleshy stem. In order to establish biotrophy with Zizania latifolia which belongs to the tribe Oryzeae (Poaceae), U. esculenta firstly needs to secrete a bunch of effectors, among them being cell wall degrading enzymes (CWDEs). We have isolated a gene, UeEgl1, which was differentially expressed in MT-type and T-type U. esculenta at an early stage of infection, and specifically induced in the filamentous growth of the T-type. Bioinformatics analysis and enzyme activity assay indicated that UeEgl1 functions outside the cell as a β-1,4-endoglucanase with a conserved domain of the glycosyl hydrolase family 45 (GH45) which targets the main component of the plant cell wall β-1,4 linked glycosidic bonds. The phenotype analysis of UeEgl1 deletion mutants and UeEgl1 over-expression transformants showed that UeEgl1 had no significant effect on the budding, cell fusion, and filamentous growth of U. esculenta in vitro. Further study found that over-expression of UeEgl1 promoted the proliferation of mycelia inside Z. latifolia, and raised plant defense responses. The above results show that the UeEgl1 gene may play an important role in the early stage of infection through the decomposition of the plant cell wall.
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Claims 7
Claim · model-written summary
UeEgl1 was differentially expressed between MT-type and T-type fungus early in infection.
- Taxon
- U. esculenta
Ustilago esculentae0 - Gene
- UeEgl1e0
- Outcome
- differentially expressede0
- Qualifier
- early stage of infectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UeEgl1, which was differentially expressed in MT-type and T-type U. esculenta at an early stage of infection”
Claim · model-written summary
UeEgl1 was induced in filamentous growth of T-type fungus.
- Taxon
- U. esculentaUstilago esculentae1
- Outcome
- specifically inducede0
- Qualifier
- filamentous growth of the T-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“specifically induced in the filamentous growth of the T-type”
e1“UeEgl1, which was differentially expressed in MT-type and T-type U. esculenta at an early stage of infection”
Claim · model-written summary
UeEgl1 encodes an extracellular β-1,4-endoglucanase targeting plant cell-wall linkages.
- Gene
- UeEgl1e0
- Outcome
- functions outside the cell as a β-1,4-endoglucanasee0
- Qualifier
- β-1,4 linked glycosidic bondse0
Not reportedTaxon
Verbatim
e0“UeEgl1 functions outside the cell as a β-1,4-endoglucanase with a conserved domain of the glycosyl hydrolase family 45 (GH45) which targets the main component of the plant cell wall β-1,4 linked glycosidic bonds”
Claim · model-written summary
UeEgl1 deletion or overexpression had no significant effect on fungal budding, fusion or filamentous growth in vitro.
- Taxon
- U. esculentaUstilago esculentae0
- Treatment
- UeEgl1 deletion mutantse0
- Outcome
- budding, cell fusion, and filamentous growthe0
- Direction
- no significant effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UeEgl1 deletion mutants and UeEgl1 over-expression transformants showed that UeEgl1 had no significant effect on the budding, cell fusion, and filamentous growth of U. esculenta in vitro”
Claim · model-written summary
UeEgl1 overexpression promoted fungal mycelial proliferation inside the host.
- Taxon
- Z. latifolia
.jpg?width=60)
Zizania latifoliae0 - Treatment
- over-expression of UeEgl1e0
- Interactor
- UeEgl1e0pathogen
- Outcome
- proliferation of myceliae0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“over-expression of UeEgl1 promoted the proliferation of mycelia inside Z. latifolia”
Claim · model-written summary
UeEgl1 overexpression raised host defense responses.
- Taxon
- Z. latifoliaZizania latifoliae0
- Treatment
- over-expression of UeEgl1e0
- Outcome
- plant defense responsese0
- Direction
- raisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“over-expression of UeEgl1 promoted the proliferation of mycelia inside Z. latifolia, and raised plant defense responses”
Claim · model-written summary
UeEgl1 may aid early infection by decomposing the plant cell wall.
Not reportedTaxon
Verbatim
e0“UeEgl1 gene may play an important role in the early stage of infection through the decomposition of the plant cell wall”