Abstract
NUCLEAR FACTOR Y subunit alpha (NF-YA), together with NF-YB and NF-YC, regulates plant growth and development, as well as plant responses to biotic and abiotic stresses. Although extensive studies have examined the functions of NF-YAs in Arabidopsis thaliana , the roles of NF- YAs in Glycinme max are poorly understood. In this study, we identified a phosphorus (P) starvation-responsive NF-YA8 in soybean. The expression of GmNF-YA8 is induced by low P or low nitrogen in leaves, but not by potassium or iron starvation, respectively. GmNF-YA8 is localized in the nucleus and plasma membrane. Ectopic expression of GmNF-YA8 inhibits plant growth and delayed flowering in Arabidopsis . Exogenous application of gibberellic acid (GA) rescues the delayed flowering phenotype in Arabidopsis overexpressing GmNF-YA8 lines GmNF-YA8OE-05 and GmNF-YA8OE-20. Moreover, quantitative real time PCR (qRT-PCR) verified that overexpression of GmNF-YA8 downregulates GA20ox2 and GA3ox2 expression, but upregulates GA2ox2 and GA2ox3 that encode enzymes, which inactive bioactive GAs. Consistent with the late flowering phenotype of Arabidopsis trangenic lines that overexpress GmNF-YA8 , the transcript levels of flowering-promoting genes AP1 , CO , LFY , and SOC1 are reduced. In addition, overexpression of GmNF-YA8 promotes the emergence of lateral root (LR) primordium from epidermis rather than the initiation of LR in low P, and increases the LR density in low nitrogen. Our results provide insights into the roles of GmNF-YA8 .
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Claims 14
Claim · model-written summary
GmNF-YA8 expression in soybean leaves is induced by low phosphorus.
- Taxon
- soybean
Glycine maxe1 - Organ / stage
- leavese2
- Treatment
- low Pe2
- Gene
- GmNF-YA8e2
- Outcome
- expressione2
- Direction
- inducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In this study, we identified a phosphorus (P) starvation-responsive NF-YA8 in soybean”
e2“The expression of GmNF-YA8 is induced by low P or low nitrogen in leaves, but not by potassium or iron starvation, respectively”
Claim · model-written summary
GmNF-YA8 expression in soybean leaves is induced by low nitrogen.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- leavese2
- Treatment
- low nitrogene2
- Gene
- GmNF-YA8e2
- Outcome
- expressione2
- Direction
- inducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In this study, we identified a phosphorus (P) starvation-responsive NF-YA8 in soybean”
e2“The expression of GmNF-YA8 is induced by low P or low nitrogen in leaves, but not by potassium or iron starvation, respectively”
Claim · model-written summary
GmNF-YA8 expression in soybean leaves is not induced by potassium starvation.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- leavese2
- Treatment
- potassiume2
- Gene
- GmNF-YA8e2
- Outcome
- expressione2
- Direction
- no_changeinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In this study, we identified a phosphorus (P) starvation-responsive NF-YA8 in soybean”
e2“The expression of GmNF-YA8 is induced by low P or low nitrogen in leaves, but not by potassium or iron starvation, respectively”
Claim · model-written summary
GmNF-YA8 expression in soybean leaves is not induced by iron starvation.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- leavese2
- Treatment
- iron starvatione2
- Gene
- GmNF-YA8e2
- Outcome
- expressione2
- Direction
- no_changeinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In this study, we identified a phosphorus (P) starvation-responsive NF-YA8 in soybean”
e2“The expression of GmNF-YA8 is induced by low P or low nitrogen in leaves, but not by potassium or iron starvation, respectively”
Claim · model-written summary
GmNF-YA8 is localized in the nucleus and plasma membrane.
- Taxon
- soybeanGlycine maxe1
- Gene
- GmNF-YA8e3
- Outcome
- localized in the nucleus and plasma membranee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In this study, we identified a phosphorus (P) starvation-responsive NF-YA8 in soybean”
e3“GmNF-YA8 is localized in the nucleus and plasma membrane”
Claim · model-written summary
Ectopic expression of GmNF-YA8 inhibits plant growth in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise4 - Treatment
- Ectopic expression of GmNF-YA8e4
- Outcome
- plant growthe4
- Direction
- inhibitse4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Ectopic expression of GmNF-YA8 inhibits plant growth and delayed flowering in Arabidopsis”
Claim · model-written summary
Ectopic expression of GmNF-YA8 delays flowering in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise4
- Treatment
- Ectopic expression of GmNF-YA8e4
- Outcome
- floweringe4
- Direction
- delayede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Ectopic expression of GmNF-YA8 inhibits plant growth and delayed flowering in Arabidopsis”
Claim · model-written summary
Exogenous gibberellic acid rescues the delayed flowering phenotype of Arabidopsis overexpressing GmNF-YA8 lines.
- Taxon
- ArabidopsisArabidopsise5
- Material
- GmNF-YA8OE-05 and GmNF-YA8OE-20e5
- Treatment
- gibberellic acide5
- Gene
- GmNF-YA8e5
- Outcome
- delayed flowering phenotypee5
- Direction
- rescuese5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Exogenous application of gibberellic acid (GA) rescues the delayed flowering phenotype in Arabidopsis overexpressing GmNF-YA8 lines GmNF-YA8OE-05 and GmNF-YA8OE-20”
Claim · model-written summary
Overexpression of GmNF-YA8 downregulates GA20ox2 and GA3ox2 expression in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise4
- Treatment
- overexpression of GmNF-YA8e6
- Gene
- GA20ox2 and GA3ox2e6
- Outcome
- expressione6
- Direction
- downregulatese6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Ectopic expression of GmNF-YA8 inhibits plant growth and delayed flowering in Arabidopsis”
e6“overexpression of GmNF-YA8 downregulates GA20ox2 and GA3ox2 expression, but upregulates GA2ox2 and GA2ox3 that encode enzymes, which inactive bioactive GAs”
Claim · model-written summary
Overexpression of GmNF-YA8 upregulates GA2ox2 and GA2ox3 expression in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise4
- Treatment
- overexpression of GmNF-YA8e6
- Gene
- GA2ox2 and GA2ox3e6
- Outcome
- expressione6
- Direction
- upregulatese6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Ectopic expression of GmNF-YA8 inhibits plant growth and delayed flowering in Arabidopsis”
e6“overexpression of GmNF-YA8 downregulates GA20ox2 and GA3ox2 expression, but upregulates GA2ox2 and GA2ox3 that encode enzymes, which inactive bioactive GAs”
Claim · model-written summary
Transcript levels of the flowering-promoting genes AP1, CO and LFY are reduced in lines that overexpress GmNF-YA8.
- Taxon
- ArabidopsisArabidopsise7
- Treatment
- lines that overexpress GmNF-YA8e7
- Gene
- AP1, CO, LFYe7
- Outcome
- transcript levelse7
- Direction
- reducede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Consistent with the late flowering phenotype of Arabidopsis trangenic lines that overexpress GmNF-YA8, the transcript levels of flowering-promoting genes AP1, CO, LFY, and SOC1 are reduced”
Claim · model-written summary
Transcript level of the flowering-promoting gene SOC1 is reduced in lines that overexpress GmNF-YA8.
- Taxon
- ArabidopsisArabidopsise7
- Treatment
- lines that overexpress GmNF-YA8e7
- Gene
- SOC1e7
- Outcome
- transcript levelse7
- Direction
- reducede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Consistent with the late flowering phenotype of Arabidopsis trangenic lines that overexpress GmNF-YA8, the transcript levels of flowering-promoting genes AP1, CO, LFY, and SOC1 are reduced”
Claim · model-written summary
Overexpression of GmNF-YA8 promotes emergence of lateral root primordium from the epidermis rather than lateral root initiation in low P.
- Taxon
- ArabidopsisArabidopsise4
- Treatment
- overexpression of GmNF-YA8e5
- Outcome
- emergence of lateral root (LR) primordium from epidermise5
- Direction
- promotese5
- Qualifier
- in low Pe5
- Comparator
- initiation of LRe5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Ectopic expression of GmNF-YA8 inhibits plant growth and delayed flowering in Arabidopsis”
e5“overexpression of GmNF-YA8 promotes the emergence of lateral root (LR) primordium from epidermis rather than the initiation of LR in low P, and increases the LR density in low nitrogen”
Claim · model-written summary
Overexpression of GmNF-YA8 increases lateral root density in low nitrogen.
- Taxon
- ArabidopsisArabidopsise4
- Treatment
- overexpression of GmNF-YA8e5
- Outcome
- LR densitye5
- Direction
- increasese5
- Qualifier
- in low nitrogene5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Ectopic expression of GmNF-YA8 inhibits plant growth and delayed flowering in Arabidopsis”
e5“overexpression of GmNF-YA8 promotes the emergence of lateral root (LR) primordium from epidermis rather than the initiation of LR in low P, and increases the LR density in low nitrogen”
The same questions in other papers
iron atom · molecular: expression · Glycine max (soybean)Positive and negative results
6 papers in the same direction as this paper · 4 in the opposite direction
This paper is about lacking the nutrient, so it counts the other way round here.
- positive
- positive
- positive
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.