Abstract
The soybean Rpp1 locus confers resistance to Phakopsora pachyrhizi, causal agent of rust, and resistance is usually dominant over susceptibility. However, dominance of Rpp1-mediated resistance is lost when a resistant genotype (Rpp1 or Rpp1b) is crossed with susceptible line TMG06_0011, and the mechanism of this dominant susceptibility (DS) is unknown. Sequencing the Rpp1 region reveals that the TMG06_0011 Rpp1 locus has a single nucleotide-binding site leucine-rich repeat (NBS-LRR) gene (DS-R), whereas resistant PI 594760B (Rpp1b) is similar to PI 200492 (Rpp1) and has three NBS-LRR resistance gene candidates. Evidence that DS-R is the cause of DS was reflected in virus-induced gene silencing of DS-R in Rpp1b/DS-R or Rpp1/DS-R heterozygous plants with resistance partially restored. In heterozygous Rpp1b/DS-R plants, expression of Rpp1b candidate genes was not significantly altered, indicating no effect of DS-R on transcription. Physical interaction of the DS-R protein with candidate Rpp1b resistance proteins was supported by yeast two-hybrid studies and in silico modeling. Thus, we conclude that suppression of resistance most likely does not occur at the transcript level, but instead probably at the protein level, possibly with Rpp1 function inhibited by binding to the DS-R protein. The DS-R gene was found in other soybean lines, with an estimated allele frequency of 6% in a diverse population, and also found in wild soybean (Glycine soja). The identification of a dominant susceptible NBS-LRR gene provides insight into the behavior of NBS-LRR proteins and serves as a reminder to breeders that the dominance of an R gene can be influenced by a susceptibility allele.
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Claims 9
Claim · model-written summary
Crosses with TMG06_0011 lost dominant Rpp1 resistance.
- Taxon
- soybean
Glycine maxt0 - Material
- TMG06_0011e0
- Interactor
- Phakopsora pachyrhiziPhakopsora pachyrhizit0pathogen
- Gene
- Rpp1e0
- Outcome
- dominance of Rpp1-mediated resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dominance of Rpp1-mediated resistance is lost when a resistant genotype (Rpp1 or Rpp1b) is crossed with susceptible line TMG06_0011”
t0“Phakopsora pachyrhizi in soybean”
Claim · model-written summary
TMG06_0011 carried one NBS-LRR gene at Rpp1.
- Taxon
- soybeanGlycine maxt0
- Material
- TMG06_0011e0
- Gene
- DS-Re0
- Outcome
- single nucleotide-binding site leucine-rich repeat (NBS-LRR) genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the TMG06_0011 Rpp1 locus has a single nucleotide-binding site leucine-rich repeat (NBS-LRR) gene (DS-R)”
t0“soybean”
Claim · model-written summary
PI 594760B had three NBS-LRR resistance gene candidates.
- Taxon
- soybeanGlycine maxt0
- Material
- PI 594760Be0
- Gene
- Rpp1be0
- Outcome
- NBS-LRR resistance gene candidatese0
- Value
- threee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“resistant PI 594760B (Rpp1b) is similar to PI 200492 (Rpp1) and has three NBS-LRR resistance gene candidates”
t0“soybean”
Claim · model-written summary
Silencing DS-R partially restored resistance in heterozygous plants.
- Taxon
- soybeanGlycine maxt0
- Treatment
- virus-induced gene silencing of DS-Re0
- Gene
- DS-Re0
- Outcome
- resistancee0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“virus-induced gene silencing of DS-R in Rpp1b/DS-R or Rpp1/DS-R heterozygous plants with resistance partially restored”
t0“soybean”
Claim · model-written summary
DS-R did not significantly change Rpp1b candidate gene expression.
- Taxon
- soybeanGlycine maxt0
- Gene
- Rpp1be0
- Outcome
- expression of Rpp1b candidate genese0
- Direction
- not significantly alterede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In heterozygous Rpp1b/DS-R plants, expression of Rpp1b candidate genes was not significantly altered”
t0“soybean”
Claim · model-written summary
DS-R physically interacted with candidate Rpp1b proteins in supporting analyses.
- Taxon
- soybeanGlycine maxt0
- Gene
- DS-R proteine0
- Outcome
- Physical interaction of the DS-R protein with candidate Rpp1b resistance proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Physical interaction of the DS-R protein with candidate Rpp1b resistance proteins was supported by yeast two-hybrid studies and in silico modeling.”
t0“soybean”
Claim · model-written summary
Authors propose that DS-R suppresses resistance at the protein level, possibly through binding.
- Taxon
- soybeanGlycine maxt0
- Gene
- DS-R proteine0
- Outcome
- suppression of resistancee0
- Qualifier
- probably at the protein levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suppression of resistance most likely does not occur at the transcript level, but instead probably at the protein level, possibly with Rpp1 function inhibited by binding to the DS-R protein”
t0“soybean”
Claim · model-written summary
DS-R occurred in other soybean lines at an estimated 6% allele frequency.
- Taxon
- soybeanGlycine maxe0
- Gene
- DS-R genee0
- Outcome
- allele frequencye0
- Value
- 6%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The DS-R gene was found in other soybean lines, with an estimated allele frequency of 6% in a diverse population”
Claim · model-written summary
DS-R was found in wild soybean.
- Taxon
- wild soybean (Glycine soja)
Glycine sojae0 - Gene
- DS-Rt0
- Outcome
- found in wild soybean (Glycine soja)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“and also found in wild soybean (Glycine soja)”
t0“The DS-R gene was found in other soybean lines”
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