Abstract
Screening and identification of tolerant genotypes using osmotic materials under in vitro culture could be rapid, easy, and even accurate. In this research, three Iranian landraces of Cucurbita sp. included Tanbal Ajili (Cucurbita maxima Duch.), Ajili Razan (Cucurbita pepo L.), and Balghabakhi (Cucurbita moschata Duch.) seeds were cultured in ¼ MS medium. After germination, plantlets were transferred to MS media containing mannitol and PEG 6000. Mannitol and PEG at three concentrations of 0.1, 0.2, and 0.4 M and 0.009, 0.012, and 0.015 M, respectively, were added into the MS medium, while the MS medium without any adding was used as control. Our findings revealed that osmotic treatments significantly increased shoot and root dry weight (DW), malondialdehyde (MDA), and proline content, but significantly reduced coleoptile length, shoot and root fresh weight, and photosynthesis pigments content. Protein content, phenols, and flavonoids content, enzymatic and non-enzymatic antioxidant including ascorbate peroxidase (APX), guaiacol peroxidase (GPX), superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) activity, reduced ascorbate (AsA), reduced ascorbate/dehydroascorbic acid (AsA/DHA), reduced glutathione (GSH), dehydroascorbic acid (DHA) and oxidized glutathione (GSSG), and reduced glutathione/oxidized glutathione (GSH/GSSG) were significantly increased at moderate osmotic stress induced by mannitol and PEG. In contrast, the previous physiological parameters were significantly reduced at higher water deficit conditions. With respect to most attributes and concentrations, mannitol simulated osmotic stress better than PEG. Our results revealed that applying PEG and mannitol under in vitro conditions could be an efficient way to evaluate and screen cucurbit genotypes for future breeding programs.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 12
Claim · model-written summary
Osmotic treatments significantly increased shoot and root dry weight.
CucurbitaNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“osmotic treatments significantly increased shoot and root dry weight (DW), malondialdehyde (MDA), and proline content”
t0“Cucurbita sp.”
Claim · model-written summary
Osmotic treatments significantly increased malondialdehyde and proline content.
- Treatment
- osmotic treatmentse0
- Outcome
- malondialdehyde (MDA), and proline contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“osmotic treatments significantly increased shoot and root dry weight (DW), malondialdehyde (MDA), and proline content”
t0“Cucurbita sp.”
Claim · model-written summary
Osmotic treatments significantly reduced coleoptile length and shoot and root fresh weight.
- Treatment
- osmotic treatmentse0
- Outcome
- coleoptile length, shoot and root fresh weighte0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“osmotic treatments significantly increased shoot and root dry weight (DW), malondialdehyde (MDA), and proline content, but significantly reduced coleoptile length, shoot and root fresh weight, and photosynthesis pigments content”
t0“Cucurbita sp.”
Claim · model-written summary
Osmotic treatments significantly reduced photosynthesis pigments content.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“osmotic treatments significantly increased shoot and root dry weight (DW), malondialdehyde (MDA), and proline content, but significantly reduced coleoptile length, shoot and root fresh weight, and photosynthesis pigments content”
t0“Cucurbita sp.”
Claim · model-written summary
Moderate osmotic stress significantly increased protein, phenol, and flavonoid content.
- Treatment
- moderate osmotic stress induced by mannitol and PEGe0
- Outcome
- Protein content, phenols, and flavonoids contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protein content, phenols, and flavonoids content, enzymatic and non-enzymatic antioxidant including ascorbate peroxidase (APX), guaiacol peroxidase (GPX), superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) activity, reduced ascorbate (AsA), reduced ascorbate/dehydroascorbic acid (AsA/DHA), reduced glutathione (GSH), dehydroascorbic acid (DHA) and oxidized glutathione (GSSG), and reduced glutathione/oxidized glutathione (GSH/GSSG) were significantly increased at moderate osmotic stress induced by mannitol and PEG”
t0“Cucurbita sp.”
Claim · model-written summary
Moderate stress significantly increased APX, GPX, and SOD antioxidant activity.
- Treatment
- moderate osmotic stress induced by mannitol and PEGe0
- Outcome
- ascorbate peroxidase (APX), guaiacol peroxidase (GPX), superoxide dismutase (SOD)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protein content, phenols, and flavonoids content, enzymatic and non-enzymatic antioxidant including ascorbate peroxidase (APX), guaiacol peroxidase (GPX), superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) activity, reduced ascorbate (AsA), reduced ascorbate/dehydroascorbic acid (AsA/DHA), reduced glutathione (GSH), dehydroascorbic acid (DHA) and oxidized glutathione (GSSG), and reduced glutathione/oxidized glutathione (GSH/GSSG) were significantly increased at moderate osmotic stress induced by mannitol and PEG”
t0“Cucurbita sp.”
Claim · model-written summary
Moderate stress significantly increased CAT and GR activity.
- Treatment
- moderate osmotic stress induced by mannitol and PEGe0
- Outcome
- catalase (CAT), glutathione reductase (GR) activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protein content, phenols, and flavonoids content, enzymatic and non-enzymatic antioxidant including ascorbate peroxidase (APX), guaiacol peroxidase (GPX), superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) activity, reduced ascorbate (AsA), reduced ascorbate/dehydroascorbic acid (AsA/DHA), reduced glutathione (GSH), dehydroascorbic acid (DHA) and oxidized glutathione (GSSG), and reduced glutathione/oxidized glutathione (GSH/GSSG) were significantly increased at moderate osmotic stress induced by mannitol and PEG”
t0“Cucurbita sp.”
Claim · model-written summary
Moderate stress significantly increased several ascorbate and glutathione redox measures.
- Treatment
- moderate osmotic stress induced by mannitol and PEGe0
- Outcome
- reduced ascorbate (AsA), reduced ascorbate/dehydroascorbic acid (AsA/DHA), reduced glutathione (GSH)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protein content, phenols, and flavonoids content, enzymatic and non-enzymatic antioxidant including ascorbate peroxidase (APX), guaiacol peroxidase (GPX), superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) activity, reduced ascorbate (AsA), reduced ascorbate/dehydroascorbic acid (AsA/DHA), reduced glutathione (GSH), dehydroascorbic acid (DHA) and oxidized glutathione (GSSG), and reduced glutathione/oxidized glutathione (GSH/GSSG) were significantly increased at moderate osmotic stress induced by mannitol and PEG”
t0“Cucurbita sp.”
Claim · model-written summary
Moderate stress significantly increased DHA, GSSG, and GSH/GSSG.
- Treatment
- moderate osmotic stress induced by mannitol and PEGe0
- Outcome
- dehydroascorbic acid (DHA) and oxidized glutathione (GSSG), and reduced glutathione/oxidized glutathione (GSH/GSSG)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protein content, phenols, and flavonoids content, enzymatic and non-enzymatic antioxidant including ascorbate peroxidase (APX), guaiacol peroxidase (GPX), superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) activity, reduced ascorbate (AsA), reduced ascorbate/dehydroascorbic acid (AsA/DHA), reduced glutathione (GSH), dehydroascorbic acid (DHA) and oxidized glutathione (GSSG), and reduced glutathione/oxidized glutathione (GSH/GSSG) were significantly increased at moderate osmotic stress induced by mannitol and PEG”
t0“Cucurbita sp.”
Claim · model-written summary
The preceding physiological parameters significantly declined at higher water deficit.
- Treatment
- higher water deficit conditionse0
- Outcome
- previous physiological parameterse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the previous physiological parameters were significantly reduced at higher water deficit conditions”
t0“Cucurbita sp.”
Claim · model-written summary
Mannitol simulated osmotic stress better than PEG for most attributes and concentrations.
- Treatment
- mannitole0
- Outcome
- simulated osmotic stresse0
- Direction
- bettere0
- Qualifier
- most attributes and concentrationse0
- Comparator
- PEGe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With respect to most attributes and concentrations, mannitol simulated osmotic stress better than PEG”
t0“Cucurbita sp.”
Claim · model-written summary
PEG and mannitol in vitro were judged efficient for screening cucurbit genotypes for breeding.
Not reportedComparatorValue
Verbatim
e0“applying PEG and mannitol under in vitro conditions could be an efficient way to evaluate and screen cucurbit genotypes for future breeding programs”
The same questions in other papers
drought · quality: protein · Cucurbita (pumpkins)Positive and negative results
1 in the opposite direction
- negative
drought · physiology: antioxidant enzymes · Cucurbita (pumpkins)Positive and negative results
1 in the opposite direction
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.