Abstract
Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is a serious fungal wheat disease of wheat worldwide. Host resistance is considered to be the most environmentally friendly and efficient approach against this disease. Wheat breeding line GR18-1 showed resistance to powdery mildew at both seedling and adult stages for several years. Genetic analysis indicated that a single dominant gene, tentatively designated as PmGR-18, conferred powdery mildew resistance in GR18-1. Bulked segregant analysis and marker analysis showed that PmGR-18 was located in the Pm4 interval on chromosome arm 2AL and was flanked by the markers Xwgrc763 and Xwgrc872, respectively, with genetic distances of 0.5 and 1.0 cM corresponding to a physical interval of 1.13 Mb based on the Chinese Spring reference genome sequence v2.1. Using homology-based cloning and Sanger sequencing, we found that the sequence of PmGR-18 was totally consistent with that of Pm4d. qRT-PCR analysis showed that the expression levels of two splicing variants Pm4d_V1 and Pm4d_V2 in GR18-1 were significantly upregulated after inoculating with Bgt isolate E09, and the level of Pm4d_V2 was significantly lower than that of Pm4d_V1 at most of the time points, suggesting a different resistance pattern may be involved in the genotype. To facilitate the transfer of PmGR-18 in marker-assisted selection (MAS) breeding, the flanked markers Xwgrc763 and Xwgrc872 and the functional marker JS717/JS718 were tested and confirmed to enable the tracking of PmGR-18 when it transferred into those susceptible cultivars.
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Claims 9
Claim · model-written summary
GR18-1 resisted powdery mildew at seedling and adult stages.
- Taxon
- Wheat
Triticum aestivumt0 - Material
- GR18-1e0
- Organ / stage
- seedling and adult stagese0
- Interactor
- Blumeria graminis f. sp. tritici
Blumeria graminis f. sp. triticie1pathogent0 - Outcome
- resistance to powdery mildewe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wheat breeding line GR18-1 showed resistance to powdery mildew at both seedling and adult stages for several years.”
t0“Wheat”
e1“Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt)”
Claim · model-written summary
A single dominant PmGR-18 gene conferred mildew resistance in GR18-1.
- Taxon
- WheatTriticum aestivumt0
- Material
- GR18-1e0
- Gene
- PmGR-18e0
- Outcome
- powdery mildew resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Genetic analysis indicated that a single dominant gene, tentatively designated as PmGR-18, conferred powdery mildew resistance in GR18-1.”
t0“Wheat”
Claim · model-written summary
PmGR-18 mapped to the Pm4 interval on chromosome arm 2AL.
- Taxon
- WheatTriticum aestivumt0
- Gene
- PmGR-18e0
- Outcome
- located in the Pm4 interval on chromosome arm 2ALe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PmGR-18 was located in the Pm4 interval on chromosome arm 2AL”
t0“Wheat”
Claim · model-written summary
Xwgrc763 and Xwgrc872 flank PmGR-18 at 0.5 and 1.0 cM, defining a 1.13 Mb interval.
- Taxon
- WheatTriticum aestivumt0
- Gene
- PmGR-18e1
- Outcome
- genetic distancese0
- Value
- 0.5 and 1.0 cMe0
- Qualifier
- physical interval of 1.13 Mbe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“flanked by the markers Xwgrc763 and Xwgrc872, respectively, with genetic distances of 0.5 and 1.0 cM corresponding to a physical interval of 1.13 Mb”
t0“Wheat”
e1“Genetic analysis indicated that a single dominant gene, tentatively designated as PmGR-18, conferred powdery mildew resistance in GR18-1.”
Claim · model-written summary
PmGR-18 sequence matched Pm4d exactly.
- Taxon
- WheatTriticum aestivumt0
- Gene
- PmGR-18e0
- Outcome
- sequence of PmGR-18e0
- Direction
- totally consistente0
- Comparator
- Pm4de0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the sequence of PmGR-18 was totally consistent with that of Pm4d”
t0“Wheat”
Claim · model-written summary
After Bgt E09 inoculation, Pm4d_V1 expression rose significantly.
- Taxon
- WheatTriticum aestivumt0
- Material
- GR18-1e0
- Treatment
- inoculating with Bgt isolate E09e0
- Interactor
- Bgt isolate E09e0pathogen
- Gene
- Pm4d_V1e0
- Outcome
- expression levelse0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression levels of two splicing variants Pm4d_V1 and Pm4d_V2 in GR18-1 were significantly upregulated after inoculating with Bgt isolate E09”
t0“Wheat”
Claim · model-written summary
After Bgt E09 inoculation, Pm4d_V2 expression rose significantly.
- Taxon
- WheatTriticum aestivumt0
- Material
- GR18-1e0
- Treatment
- inoculating with Bgt isolate E09e0
- Interactor
- Bgt isolate E09e0pathogen
- Gene
- Pm4d_V2e0
- Outcome
- expression levelse0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression levels of two splicing variants Pm4d_V1 and Pm4d_V2 in GR18-1 were significantly upregulated after inoculating with Bgt isolate E09”
t0“Wheat”
Claim · model-written summary
Pm4d_V2 expression was significantly lower than Pm4d_V1 at most time points.
- Taxon
- WheatTriticum aestivumt0
- Gene
- Pm4d_V2e0
- Outcome
- level of Pm4d_V2e0
- Direction
- lowere0
- Qualifier
- at most of the time pointse0
- Comparator
- Pm4d_V1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the level of Pm4d_V2 was significantly lower than that of Pm4d_V1 at most of the time points”
t0“Wheat”
Claim · model-written summary
Flanking and functional markers enabled tracking PmGR-18 into susceptible cultivars.
- Taxon
- WheatTriticum aestivumt0
- Gene
- PmGR-18e0
- Outcome
- tracking of PmGR-18e0
- Comparator
- susceptible cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the flanked markers Xwgrc763 and Xwgrc872 and the functional marker JS717/JS718 were tested and confirmed to enable the tracking of PmGR-18 when it transferred into those susceptible cultivars”
t0“Wheat”
The same questions in other papers
inoculation · molecular: expression · Triticum aestivum (wheat)Positive and negative results
37 papers in the same direction as this paper · 9 in the opposite direction
- no effect
- no effect
- no effect
43 papers more in this group
See all 47 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.