Abstract
Ustilago maydis expresses a number of proteases during its pathogenic lifecycle. Some of the proteases including both intracellular and extracellular ones have previously been shown to influence the virulence of the pathogen. However, any role of secreted proteases in the sporulation process of U. maydis have not been explored earlier. In this study we have investigated the biological function of one such secreted protease, Ger1 belonging to aspartic protease A1 family. An assessment of the real time expression of ger1 revealed an infection specific expression of the protein especially during late phases of infection. We also evaluated any contribution of the protein in the pathogenicity of the fungus. Our data revealed an involvement of Ger1 in the sporulation and spore germination processes of U. maydis. Ger1 also showed positive influence on the pathogenicity of the fungus and accordingly the ger1 deletion mutant exhibited reduced pathogenicity. The study also demonstrated the protease activity associated with Ger1 to be essential for its biological function. Fluorescence microscopy of maize plants infected with U. maydis cells expressing Ger1-mcherry-HA also revealed that Ger1 is efficiently secreted within maize apoplast.
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Claims 6
Claim · model-written summary
Ger1 expression was specific to infection and especially late infection.
- Taxon
- Ustilago maydis
Mycosarcoma maydist0 - Organ / stage
- late phases of infectione0
- Gene
- Ger1t0
- Outcome
- infection specific expressione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ger1 is a secreted aspartic acid protease essential for spore germination in Ustilago maydis”
e0“an infection specific expression of the protein especially during late phases of infection”
Claim · model-written summary
Ger1 was involved in sporulation and spore germination.
- Taxon
- Ustilago maydisMycosarcoma maydist0
- Gene
- Ger1e0
- Outcome
- sporulation and spore germination processese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ger1 is a secreted aspartic acid protease essential for spore germination in Ustilago maydis”
e0“an involvement of Ger1 in the sporulation and spore germination processes of U. maydis”
Claim · model-written summary
Ger1 positively influenced fungal pathogenicity.
- Taxon
- Ustilago maydisMycosarcoma maydist0
- Gene
- Ger1e0
- Outcome
- pathogenicity of the funguse0
- Direction
- positive influencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ger1 is a secreted aspartic acid protease essential for spore germination in Ustilago maydis”
e0“Ger1 also showed positive influence on the pathogenicity of the fungus”
Claim · model-written summary
The ger1 deletion mutant had reduced pathogenicity.
- Taxon
- Ustilago maydisMycosarcoma maydist0
- Material
- ger1 deletion mutante0
- Outcome
- pathogenicitye0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ger1 is a secreted aspartic acid protease essential for spore germination in Ustilago maydis”
e0“the ger1 deletion mutant exhibited reduced pathogenicity”
Claim · model-written summary
Ger1 protease activity was essential for its biological function.
- Taxon
- Ustilago maydisMycosarcoma maydist0
- Gene
- Ger1e0
- Outcome
- biological functione0
- Qualifier
- protease activitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ger1 is a secreted aspartic acid protease essential for spore germination in Ustilago maydis”
e0“the protease activity associated with Ger1 to be essential for its biological function”
Claim · model-written summary
Ger1 was secreted into the apoplast of infected maize.
- Organ / stage
- maize apoplaste0
- Interactor
- U. maydisMycosarcoma maydise0pathogen
- Gene
- Ger1-mcherry-HAe0
- Outcome
- Ger1 is efficiently secreted within maize apoplaste0
Zea maysNot reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maize plants infected with U. maydis cells expressing Ger1-mcherry-HA also revealed that Ger1 is efficiently secreted within maize apoplast”