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Organism: Mycosarcoma maydis ✕

Observationobservedoriginal resultmeasured

Claim · model-written summary

About one third of fungal genes affected by Nit2 during biotrophy participate in nitrogen metabolism and transport, with minor overlap to sporidia.

Taxon
Ustilago maydisMycosarcoma maydise1
Gene
Nit2e0
Outcome
fungal genes affected by Nit2e0
Value
About one thirde0
Qualifier
involved in nitrogen metabolism and transport, only showing minor overlap to saprotrophic sporidiae0

Verbatim

e0

“About one third of the fungal genes affected by Nit2 during biotrophy were involved in nitrogen metabolism and transport, only showing minor overlap to saprotrophic sporidia.”

✓ located verbatim in the source · abstract, characters 687–861
e1

“corn smut fungus Ustilago maydis”

✓ located verbatim in the source · abstract, characters 231–263

Title

Ustilago maydis Nit2 Regulates Nitrate Utilisation During Biotrophy and Affects Amino Acid Metabolism of Galls Under Nitrogen Depletion

Philipp L. Lopinski; Christin Schulz; Alicia Fischer; et al. Molecular Plant Pathology 2025. doi:10.1111/mpp.70148

Other claims from this paper (6)
Mechanismobservedoriginal resultmeasured

Claim · model-written summary

Induction of the nitrate assimilation cluster during biotrophy is completely Nit2-dependent.

Taxon
Ustilago maydisMycosarcoma maydise1
Gene
Nit2e0
Outcome
Induction of the nitrate assimilation clustere0
Qualifier
during biotrophye0

Verbatim

e0

“Induction of the nitrate assimilation cluster was completely dependent on Nit2 during biotrophy.”

✓ located verbatim in the source · abstract, characters 862–958
e1

“corn smut fungus Ustilago maydis”

✓ located verbatim in the source · abstract, characters 231–263

Title

Ustilago maydis Nit2 Regulates Nitrate Utilisation During Biotrophy and Affects Amino Acid Metabolism of Galls Under Nitrogen Depletion

Philipp L. Lopinski; Christin Schulz; Alicia Fischer; et al. Molecular Plant Pathology 2025. doi:10.1111/mpp.70148

Other claims from this paper (6)
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Pathogenicity was comparable between fungal genotypes in both nitrogen regimes.

Taxon
Ustilago maydisMycosarcoma maydise3
Treatment
Δnit2e1
Interactor
cornZea mays subsp. mayse3host
Outcome
pathogenicitye0
Direction
comparablee0
Qualifier
in both nitrogen regimese0
Comparator
wild-typee2

Verbatim

e0

“However, total protein content in galls and pathogenicity were comparable between fungal genotypes in both nitrogen regimes.”

✓ located verbatim in the source · abstract, characters 1165–1289
e1

“Δnit2 leaf galls”

✓ located verbatim in the source · abstract, characters 992–1008
e2

“wild-type galls”

✓ located verbatim in the source · abstract, characters 1148–1163
e3

“corn smut fungus Ustilago maydis”

✓ located verbatim in the source · abstract, characters 231–263

Title

Ustilago maydis Nit2 Regulates Nitrate Utilisation During Biotrophy and Affects Amino Acid Metabolism of Galls Under Nitrogen Depletion

Philipp L. Lopinski; Christin Schulz; Alicia Fischer; et al. Molecular Plant Pathology 2025. doi:10.1111/mpp.70148

Other claims from this paper (6)
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The authors conclude nitrate utilisation is dispensable during U. maydis biotrophy and likely compensated by increased organic nitrogen utilisation, partly dependent on Nit2.

Taxon
U. maydisMycosarcoma maydise0
Gene
Nit2e0
Compound
asparagine, GABA and glutaminee0
Outcome
nitrate utilisation is dispensablee0
Qualifier
can likely be actively compensated by increased utilisation of abundant organic nitrogen sourcese0

Verbatim

e0

“nitrate utilisation is dispensable for U. maydis during biotrophy and can likely be actively compensated by increased utilisation of abundant organic nitrogen sources, like asparagine, GABA and glutamine in a partially Nit2-dependent fashion.”

✓ located verbatim in the source · abstract, characters 1369–1611

Title

Ustilago maydis Nit2 Regulates Nitrate Utilisation During Biotrophy and Affects Amino Acid Metabolism of Galls Under Nitrogen Depletion

Philipp L. Lopinski; Christin Schulz; Alicia Fischer; et al. Molecular Plant Pathology 2025. doi:10.1111/mpp.70148

Other claims from this paper (6)
Observationobservedoriginal resultmeasured

Claim · model-written summary

Comparative secretome analysis of 11 Ustilaginaceae species identified 53 core homologous groups.

Taxon
Ustilago maydisMycosarcoma maydist0
Outcome
core homologous groupse0
Value
53e0
Qualifier
commonly present in this lineagee0

Verbatim

t0

“Contribute to the Virulence ofUstilago maydis”

✓ located verbatim in the source · title, characters 52–97
e0

“By analyzing the secretomes of 11 species within Ustilaginaceae, we identified 53 core homologous groups commonly present in this lineage”

✓ located verbatim in the source · abstract, characters 445–582

Title

Novel Secreted Effectors Conserved Among Smut Fungi Contribute to the Virulence ofUstilago maydis

Mariana Schuster; Gabriel Schweizer; Stefanie Reißmann; et al. Molecular Plant-Microbe Interactions 2024. doi:10.1094/mpmi-09-23-0139-fi

Other claims from this paper (4)
Observationobservedoriginal resultmeasured

Claim · model-written summary

Of 53 mutant strains lacking core effectors, 20 were affected in virulence.

Taxon
Ustilago maydisMycosarcoma maydist0
Material
mutant strainse1
Outcome
affected in virulencee1
Value
20 of these 53e1

Verbatim

t0

“Contribute to the Virulence ofUstilago maydis”

✓ located verbatim in the source · title, characters 52–97
e1

“Pathogenicity assays revealed that 20 of these 53 mutant strains were affected in virulence”

✓ located verbatim in the source · abstract, characters 799–890

Title

Novel Secreted Effectors Conserved Among Smut Fungi Contribute to the Virulence ofUstilago maydis

Mariana Schuster; Gabriel Schweizer; Stefanie Reißmann; et al. Molecular Plant-Microbe Interactions 2024. doi:10.1094/mpmi-09-23-0139-fi

Other claims from this paper (4)
Observationobservedoriginal resultmeasured

Claim · model-written summary

Among 33 mutants with no obvious phenotypic change, 13 carried additional sequence-divergent paralogs.

Taxon
Ustilago maydisMycosarcoma maydist0
Outcome
paralogse2
Value
13e2
Qualifier
33 mutants that had no obvious phenotypic changese2

Verbatim

t0

“Contribute to the Virulence ofUstilago maydis”

✓ located verbatim in the source · title, characters 52–97
e2

“Among the 33 mutants that had no obvious phenotypic changes, 13 carried additional, sequence-divergent, structurally similar paralogs”

✓ located verbatim in the source · abstract, characters 892–1025

Title

Novel Secreted Effectors Conserved Among Smut Fungi Contribute to the Virulence ofUstilago maydis

Mariana Schuster; Gabriel Schweizer; Stefanie Reißmann; et al. Molecular Plant-Microbe Interactions 2024. doi:10.1094/mpmi-09-23-0139-fi

Other claims from this paper (4)
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Seven previously uncharacterized single core effectors contribute to virulence of Ustilago maydis.

Taxon
Ustilago maydisMycosarcoma maydist0
Gene
previously uncharacterized single core effectorse3
Outcome
virulence contributione3
Value
sevene3

Verbatim

t0

“Contribute to the Virulence ofUstilago maydis”

✓ located verbatim in the source · title, characters 52–97
e3

“We report a virulence contribution of seven previously uncharacterized single core effectors and of one effector family”

✓ located verbatim in the source · abstract, characters 1027–1146

Title

Novel Secreted Effectors Conserved Among Smut Fungi Contribute to the Virulence ofUstilago maydis

Mariana Schuster; Gabriel Schweizer; Stefanie Reißmann; et al. Molecular Plant-Microbe Interactions 2024. doi:10.1094/mpmi-09-23-0139-fi

Other claims from this paper (4)
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

One conserved effector family contributes to virulence of Ustilago maydis.

Taxon
Ustilago maydisMycosarcoma maydist0
Gene
effector familye3
Outcome
virulence contributione3
Value
onee3

Verbatim

t0

“Contribute to the Virulence ofUstilago maydis”

✓ located verbatim in the source · title, characters 52–97
e3

“We report a virulence contribution of seven previously uncharacterized single core effectors and of one effector family”

✓ located verbatim in the source · abstract, characters 1027–1146

Title

Novel Secreted Effectors Conserved Among Smut Fungi Contribute to the Virulence ofUstilago maydis

Mariana Schuster; Gabriel Schweizer; Stefanie Reißmann; et al. Molecular Plant-Microbe Interactions 2024. doi:10.1094/mpmi-09-23-0139-fi

Other claims from this paper (4)
Observationobservedoriginal resultmeasured

Claim · model-written summary

Ger1 expression was specific to infection and especially late infection.

Taxon
Ustilago maydisMycosarcoma maydist0
Organ / stage
late phases of infectione0
Gene
Ger1t0
Outcome
infection specific expressione0

Not reportedDirection

Verbatim

t0

“Ger1 is a secreted aspartic acid protease essential for spore germination in Ustilago maydis”

✓ located verbatim in the source · title, characters 0–92
e0

“an infection specific expression of the protein especially during late phases of infection”

✓ located verbatim in the source · abstract, characters 534–624

Title

Ger1 is a secreted aspartic acid protease essential for spore germination in Ustilago maydis

Subhasish Mukherjee; Koustav Bhakta; Abhrajyoti Ghosh; et al. Yeast 2022. doi:10.1002/yea.3835

Other claims from this paper (5)

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