Abstract
Viruses are strict intracellular parasites that rely on the proteins encoded in their genomes for the effective manipulation of the infected cell that ultimately enables a successful infection. Viral proteins have to be produced during the cell invasion and takeover in sufficient amounts and in a timely manner. Silencing suppressor proteins evolved by plant viruses can boost the production of viral proteins; although, additional mechanisms for the regulation of viral protein production likely exist. The strongest silencing suppressor encoded by the geminivirus tomato yellow leaf curl virus (TYLCV) is V2: V2 suppresses both post-transcriptional and transcriptional gene silencing (PTGS and TGS), activities that are associated with its localization in punctate cytoplasmic structures and in the nucleus, respectively. However, V2 has been previously described to largely localize in the endoplasmic reticulum (ER), although the biological relevance of this distribution remains mysterious. Here, we confirm the association of V2 to the ER in Nicotiana benthamiana and assess the silencing suppression activity-independent impact of V2 on protein accumulation. Our results indicate that V2 has no obvious influence on the localization of ER-synthesized receptor-like kinases (RLKs) or ER quality control (ERQC)/ER-associated degradation (ERAD), but dramatically enhances the accumulation of the viral C4 protein, which is co-translationally myristoylated, possibly in proximity to the ER. By using the previously described V2C84S/86S mutant, in which the silencing suppression activity is abolished, we uncouple RNA silencing from the observed effect. Therefore, this work uncovers a novel function of V2, independent of its capacity to suppress silencing, in the promotion of the accumulation of another crucial viral protein.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
V2 associates with the endoplasmic reticulum in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Interactor
- Tomato Yellow Leaf Curl VirusTomato yellow leaf curl virust0pathogen
- Gene
- V2e1
- Outcome
- association of V2 to the ERe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“V2 Protein from the Geminivirus Tomato Yellow Leaf Curl Virus”
e1“Here, we confirm the association of V2 to the ER in Nicotiana benthamiana”
Claim · model-written summary
V2 had no obvious influence on the localization of ER-synthesized receptor-like kinases.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- V2e2
- Gene
- V2e2
- Outcome
- localization of ER-synthesized receptor-like kinasese2
- Direction
- no obvious influencee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here, we confirm the association of V2 to the ER in Nicotiana benthamiana”
e2“V2 has no obvious influence on the localization of ER-synthesized receptor-like kinases (RLKs) or ER quality control (ERQC)/ER-associated degradation (ERAD)”
Claim · model-written summary
V2 had no obvious influence on ER quality control or ER-associated degradation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- V2e2
- Gene
- V2e2
- Outcome
- ER quality control (ERQC)/ER-associated degradation (ERAD)e2
- Direction
- no obvious influencee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here, we confirm the association of V2 to the ER in Nicotiana benthamiana”
e2“V2 has no obvious influence on the localization of ER-synthesized receptor-like kinases (RLKs) or ER quality control (ERQC)/ER-associated degradation (ERAD)”
Claim · model-written summary
V2 dramatically enhanced the accumulation of the viral C4 protein.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- V2e2
- Gene
- C4e3
- Outcome
- accumulation of the viral C4 proteine3
- Direction
- dramatically enhancese3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here, we confirm the association of V2 to the ER in Nicotiana benthamiana”
e2“V2 has no obvious influence on the localization of ER-synthesized receptor-like kinases (RLKs) or ER quality control (ERQC)/ER-associated degradation (ERAD)”
e3“dramatically enhances the accumulation of the viral C4 protein”
Claim · model-written summary
The enhancement of C4 accumulation by V2 is independent of its silencing suppression activity.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Material
- V2C84S/86S mutante4
- Outcome
- observed effecte4
- Qualifier
- silencing suppression activity is abolishede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here, we confirm the association of V2 to the ER in Nicotiana benthamiana”
e4“By using the previously described V2C84S/86S mutant, in which the silencing suppression activity is abolished, we uncouple RNA silencing from the observed effect”