Abstract
In this study, some isolates of Alternaria solani; the causative agent of potato early blight disease were isolated and identified from different Egyptian governorates. The pathogenicity of these isolates was examined on the potato cultivar (Lady-Rosetta). The genetic variation among these isolates were identified using the RAPD and SDS-PAGE techniques. Also, the antifungal activity of some biological agents against the highest aggressive isolate of El-Minia governorate (El-Borgia No.1) was evaluated in the laboratory, the greenhouse and the field. Five isolates were isolated from leaves and tubers of naturally infected potato plants, collected from fields planted with the varieties (Kara, Lady-Rosetta and Spunta) representing different localities of some governorates. The fungal isolates were symbolized from 1 to 5 according to their regions. Data showed that all tested isolates proved to be pathogenic to the tested potato (cv. Lady-Rosetta) causing symptoms of early blight comparing with the control. The fungal isolate No. 1 which was obtained from (El-Borgia, Minia governorate) was the most aggressive isolate with the highest significant values of disease severity (44.30 and 56.19%) of the two successive seasons, respectively. Regarding to, RAPD-PCR, SDS-PAGE protein patterns and phylogenetic analysis data showed that isolates 1, 2 and 3 that have the high pathogenicity were similar to each other and share the same cluster while the other fungal isolates 4 and 5 with the low pathogenicity were phylogenetically close to each other. Moreover, there were no relationship between the pathogenicity and the geographic distribution of all tested A. solani isolates. Finally, in vitro antagonistic test declared that Trichoderma harzianum was the best bioagent that gave the highest inhibition zone (67.33cm) against the most aggressive A. solani isolates as compared with all other bioagents. Spraying the sensitive potato plants (cv. Lady Rosetta) in the greenhouse and field antagonistic with T. harzianum suspension showed the best results and gave the lowest values of early blight disease severity during the two seasons (19.77, 11.25%) and (25.32 and 10.66%, respectively) as compared with the control and other treatments.
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Claims 9
Claim · model-written summary
All tested A. solani isolates caused early blight symptoms on Lady-Rosetta potato relative to control.
- Taxon
- potato
Solanum tuberosume0 - Material
- Lady-Rosettae0
- Treatment
- tested isolatese0
- Interactor
- isolatese0pathogen
- Outcome
- symptoms of early blighte0
- Comparator
- controle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all tested isolates proved to be pathogenic to the tested potato (cv. Lady-Rosetta) causing symptoms of early blight comparing with the control”
Claim · model-written summary
Isolate No. 1 from El-Borgia had the greatest disease severity, 44.30% in one season.
- Interactor
- fungal isolate No. 1e0pathogen
- Outcome
- disease severitye0
- Direction
- higheste0
- Value
- 44.30e0
- Setting
- El-Borgia, Minia governoratee0
Not reportedTaxon
Verbatim
e0“fungal isolate No. 1 which was obtained from (El-Borgia, Minia governorate) was the most aggressive isolate with the highest significant values of disease severity (44.30 and 56.19%) of the two successive seasons”
Claim · model-written summary
Isolate No. 1 had 56.19% disease severity in the second season.
Not reportedTaxon
Verbatim
e0“highest significant values of disease severity (44.30 and 56.19%) of the two successive seasons”
Claim · model-written summary
High-pathogenicity isolates 1, 2 and 3 clustered together.
Not reportedTaxon
Verbatim
e0“isolates 1, 2 and 3 that have the high pathogenicity were similar to each other and share the same cluster”
Claim · model-written summary
Low-pathogenicity isolates 4 and 5 were phylogenetically close.
Not reportedTaxon
Verbatim
e0“fungal isolates 4 and 5 with the low pathogenicity were phylogenetically close to each other”
Claim · model-written summary
Pathogenicity was unrelated to geographic distribution among tested isolates.
- Interactor
- A. solani
Alternaria solanie0pathogen - Outcome
- pathogenicitye0
- Setting
- geographic distributione0
Not reportedTaxonDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there were no relationship between the pathogenicity and the geographic distribution of all tested A. solani isolates”
Claim · model-written summary
Trichoderma harzianum gave the largest in vitro inhibition zone, 67.33cm, against aggressive A. solani.
- Treatment
- Trichoderma harzianume0
- Interactor
- A. solaniAlternaria solanie0pathogen
- Second interactor
- Trichoderma harzianum
Trichoderma harzianume0biocontrol_agent - Outcome
- inhibition zonee0
- Direction
- higheste0
- Value
- 67.33cme0
- Comparator
- all other bioagentse0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Trichoderma harzianum was the best bioagent that gave the highest inhibition zone (67.33cm) against the most aggressive A. solani isolates as compared with all other bioagents”
Claim · model-written summary
T. harzianum spraying gave the lowest early blight severity, 19.77 and 11.25% in greenhouse seasons.
- Taxon
- potatoSolanum tuberosume0
- Material
- Lady Rosettae0
- Treatment
- T. harzianum suspensione0
- Interactor
- early blighte0pathogen
- Second interactor
- T. harzianumTrichoderma harzianume0biocontrol_agent
- Outcome
- early blight disease severitye0
- Direction
- loweste0
- Value
- 19.77, 11.25%e0
- Comparator
- control and other treatmentse0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Spraying the sensitive potato plants (cv. Lady Rosetta) in the greenhouse and field antagonistic with T. harzianum suspension showed the best results and gave the lowest values of early blight disease severity during the two seasons (19.77, 11.25%) and (25.32 and 10.66%, respectively) as compared with the control and other treatments”
Claim · model-written summary
T. harzianum spraying gave the lowest field early blight severity, 25.32 and 10.66% in successive seasons.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- T. harzianum suspensione0
- Interactor
- early blighte0pathogen
- Second interactor
- T. harzianumTrichoderma harzianume0biocontrol_agent
- Outcome
- early blight disease severitye0
- Direction
- loweste0
- Value
- 25.32 and 10.66%e0
- Comparator
- control and other treatmentse0
- Setting
- fielde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Spraying the sensitive potato plants (cv. Lady Rosetta) in the greenhouse and field antagonistic with T. harzianum suspension showed the best results and gave the lowest values of early blight disease severity during the two seasons (19.77, 11.25%) and (25.32 and 10.66%, respectively) as compared with the control and other treatments”
The same questions in other papers
Trichoderma harzianum · Alternaria solani · Solanum tuberosum (potato)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Trichoderma harzianum · disease · Solanum tuberosum (potato)Results agree
8 papers in the same direction as this paper
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.