Abstract
Target of rapamycin (TOR) regulates essential processes associated with plant growth, development, and cell death by modulating metabolic activities and translation in response to environmental signals. The ATP-competitive TOR inhibitor AZD8055 suppressed the hypersensitive response (HR) cell death in Nicotiana benthamiana infected with the incompatible Ralstonia solanacearum. The induced expression of the HR marker gene hin1 was also inhibited by the AZD8055 treatment. To further clarify the mechanisms underlying TOR-regulated HR cell death, we focused on TOR-related ErbB3-binding protein 1 (EBP1) in N. benthamiana (NbEBP1). We found four EBP1 orthologs in the N. benthamiana genome. The expression levels of all four EBP1 orthologs in N. benthamiana were up-regulated by the R. solanacearum infection. The silencing of the four NbEBP1 orthologs suppressed the induction of HR cell death, hin1 expression, and the production of reactive oxygen species. These results suggest that the TOR signaling pathway helps regulate HR cell death along with reactive oxygen species-related signaling in N. benthamiana.
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Claims 6
Claim · model-written summary
AZD8055 suppressed hypersensitive response cell death in infected N. benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- AZD8055e0
- Interactor
- Ralstonia solanacearum
Ralstonia solanacearume0pathogen - Outcome
- hypersensitive response (HR) cell deathe0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ATP-competitive TOR inhibitor AZD8055 suppressed the hypersensitive response (HR) cell death in Nicotiana benthamiana infected with the incompatible Ralstonia solanacearum.”
Claim · model-written summary
AZD8055 inhibited induced hin1 expression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- AZD8055 treatmente0
- Gene
- hin1e0
- Outcome
- induced expression of the HR marker gene hin1e0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The induced expression of the HR marker gene hin1 was also inhibited by the AZD8055 treatment.”
t0“Nicotiana benthamiana”
Claim · model-written summary
Four EBP1 orthologs were found in the N. benthamiana genome.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Gene
- EBP1e0
- Outcome
- EBP1 orthologse0
- Value
- foure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We found four EBP1 orthologs in the N. benthamiana genome.”
Claim · model-written summary
R. solanacearum infection upregulated all four EBP1 orthologs.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- R. solanacearum infectione0
- Interactor
- R. solanacearumRalstonia solanacearume0pathogen
- Gene
- EBP1e0
- Outcome
- expression levels of all four EBP1 orthologse0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression levels of all four EBP1 orthologs in N. benthamiana were up-regulated by the R. solanacearum infection.”
Claim · model-written summary
Silencing NbEBP1 orthologs suppressed HR cell death, hin1 expression, and reactive oxygen species production.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- silencing of the four NbEBP1 orthologse0
- Gene
- NbEBP1e0
- Outcome
- induction of HR cell death, hin1 expression, and the production of reactive oxygen speciese0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The silencing of the four NbEBP1 orthologs suppressed the induction of HR cell death, hin1 expression, and the production of reactive oxygen species.”
t0“Nicotiana benthamiana”
Claim · model-written summary
The authors infer TOR signaling regulates HR cell death together with reactive oxygen species signaling.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Outcome
- HR cell deathe0
- Qualifier
- TOR signaling pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results suggest that the TOR signaling pathway helps regulate HR cell death along with reactive oxygen species-related signaling in N. benthamiana.”
The same questions in other papers
loss of function · disease · Nicotiana benthamianaPositive and negative results
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- positive
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