Abstract
We have been isolating and characterizing Ralstonia solanacearum-responsive genes (RsRGs) in Nicotiana plants. In this study we focused on RsRG308, which we renamed NbTCTP (N. benthamiana translationally controlled tumor protein) because it encodes a polypeptide showing similarity to translationally controlled tumor proteins. Induction of the hypersensitive response (HR) was accelerated in NbTCTP-silenced N. benthamiana plants challenged with R. solanacearum 8107 (Rs8107). The Rs8107 population decreased significantly, whereas hin1 gene expression was enhanced in the silenced plant. Accelerated induction of HR was observed in NbTCTP-silenced plants inoculated with Pseudomonas cichorii and P. syringae pv. syringae. Silencing of NbTCTP also accelerated the induction of HR cell death by Agrobacterium-mediated transient expression of HR inducers, such as AvrA, BAX, INF1 and NbMEK2(DD). NbTCTP silencing enhanced NbrbohB- and NbMEK2-mediated reactive oxygen species production, leading to HR. Transient expression of both the full-length sequence and the Bcl-xL domain of NbTCTP decreased HR cell death induced by Agrobacterium-mediated transient expression of HR inducers. NbTCTP-silenced plants also showed slightly dwarf phenotypes. Therefore, NbTCTP might have a role in cell death regulation during HR to fine-tune programmed cell death-associated plant defense responses.
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Claims 10
Claim · model-written summary
NbTCTP silencing accelerated HR after Ralstonia challenge.
- Taxon
- N. benthamiana
Nicotiana benthamianae0 - Treatment
- NbTCTP-silencede0
- Interactor
- R. solanacearum 8107
Ralstonia solanacearume0pathogen - Gene
- NbTCTPe0
- Outcome
- Induction of the hypersensitive response (HR)e0
- Direction
- acceleratede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Induction of the hypersensitive response (HR) was accelerated in NbTCTP-silenced N. benthamiana plants challenged with R. solanacearum 8107 (Rs8107).”
Claim · model-written summary
Ralstonia population fell significantly in NbTCTP-silenced plants.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- silenced plante0
- Interactor
- Rs8107e0pathogen
- Outcome
- Rs8107 populatione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Rs8107 population decreased significantly, whereas hin1 gene expression was enhanced in the silenced plant.”
t0“Nicotiana benthamiana”
Claim · model-written summary
hin1 expression increased in the silenced plant.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- silenced plante0
- Gene
- hin1e0
- Outcome
- hin1 gene expressione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Rs8107 population decreased significantly, whereas hin1 gene expression was enhanced in the silenced plant.”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbTCTP silencing accelerated HR after Pseudomonas cichorii inoculation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbTCTP-silencede0
- Interactor
- Pseudomonas cichorii
.jpg?width=60)
Pseudomonas cichoriie0pathogen - Outcome
- induction of HRe0
- Direction
- Acceleratede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Accelerated induction of HR was observed in NbTCTP-silenced plants inoculated with Pseudomonas cichorii and P. syringae pv. syringae.”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbTCTP silencing accelerated HR after P. syringae inoculation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbTCTP-silencede0
- Interactor
- P. syringae pv. syringae
Pseudomonas syringae pv. syringaee0pathogen - Outcome
- induction of HRe0
- Direction
- Acceleratede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Accelerated induction of HR was observed in NbTCTP-silenced plants inoculated with Pseudomonas cichorii and P. syringae pv. syringae.”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbTCTP silencing accelerated HR cell death induced by transient expression of HR inducers.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Silencing of NbTCTPe0
- Outcome
- induction of HR cell deathe0
- Direction
- acceleratede0
- Qualifier
- AvrA, BAX, INF1 and NbMEK2(DD)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of NbTCTP also accelerated the induction of HR cell death by Agrobacterium-mediated transient expression of HR inducers, such as AvrA, BAX, INF1 and NbMEK2(DD).”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbTCTP silencing enhanced NbrbohB- and NbMEK2-mediated ROS production.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbTCTP silencinge0
- Outcome
- NbrbohB- and NbMEK2-mediated reactive oxygen species productione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbTCTP silencing enhanced NbrbohB- and NbMEK2-mediated reactive oxygen species production, leading to HR.”
t0“Nicotiana benthamiana”
Claim · model-written summary
Transient expression of full-length NbTCTP decreased inducer-triggered HR cell death.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- full-length sequencee0
- Gene
- NbTCTPe0
- Outcome
- HR cell deathe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of both the full-length sequence and the Bcl-xL domain of NbTCTP decreased HR cell death induced by Agrobacterium-mediated transient expression of HR inducers.”
t0“Nicotiana benthamiana”
Claim · model-written summary
Transient expression of the NbTCTP Bcl-xL domain decreased inducer-triggered HR cell death.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Bcl-xL domain of NbTCTPe0
- Outcome
- HR cell deathe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of both the full-length sequence and the Bcl-xL domain of NbTCTP decreased HR cell death induced by Agrobacterium-mediated transient expression of HR inducers.”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbTCTP-silenced plants were slightly dwarf.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Material
- NbTCTP-silenced plantse0
- Outcome
- slightly dwarf phenotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbTCTP-silenced plants also showed slightly dwarf phenotypes.”
t0“Nicotiana benthamiana”
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