Abstract
Phospholipid signaling plays an important role in plant immune responses. Here, we isolated two phospholipase C4 (PLC4) orthologs in the Nicotiana benthamiana genome, designated as N. benthamiana PLC4-1 and PLC4-2 (NbPLC4-1 and NbPLC4-2). We created NbPLC4-1- and NbPLC4-2- silenced plants. Induction of the hypersensitive response (HR), including HR cell death and bacterial population reduction, was accelerated in both NbPLC4-1- and NbPLC4-2-silenced plants challenged with N. benthamiana-incompatible Ralstonia solanacearum 8107. The NbPLC4-1- and NbPLC4-2-silenced plants also showed enhanced expression of Nbhin1, a HR marker gene. Expressions of genes for salicylic acid (SA) and jasmonic acid (JA) signaling were drastically increased in NbPLC4-1- and NbPLC4-2-silenced plants by R. solanacearum inoculation. In addition, NbPLC4-1 and NbPLC4-2 silencing triggered reactive oxygen species (ROS) hyper-production. These results suggest that NbPLC4s are closely associated with JA, SA, and ROS signaling and act as negative regulators of the HR in N. benthamiana.
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Claims 7
Claim · model-written summary
Two PLC4 orthologs were isolated from the Nicotiana benthamiana genome.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Gene
- NbPLC4-1 and NbPLC4-2e1
- Outcome
- phospholipase C4 (PLC4) orthologse1
- Value
- twoe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e1“we isolated two phospholipase C4 (PLC4) orthologs in the Nicotiana benthamiana genome, designated as N. benthamiana PLC4-1 and PLC4-2 (NbPLC4-1 and NbPLC4-2)”
Claim · model-written summary
NbPLC4-1 and NbPLC4-2 silenced plants showed accelerated hypersensitive response, cell death and bacterial population reduction when challenged with incompatible Ralstonia solanacearum 8107.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbPLC4-1- and NbPLC4-2-silencede1
- Interactor
- Ralstonia solanacearum 8107
Ralstonia solanacearume1pathogen - Outcome
- HR cell death and bacterial population reductione1
- Direction
- acceleratede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e1“Induction of the hypersensitive response (HR), including HR cell death and bacterial population reduction, was accelerated in both NbPLC4-1- and NbPLC4-2-silenced plants challenged with N. benthamiana-incompatible Ralstonia solanacearum 8107”
Claim · model-written summary
NbPLC4-1 and NbPLC4-2 silenced plants showed enhanced expression of the HR marker gene Nbhin1.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbPLC4-1- and NbPLC4-2-silencede1
- Gene
- Nbhin1e1
- Outcome
- expression of Nbhin1e1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e1“The NbPLC4-1- and NbPLC4-2-silenced plants also showed enhanced expression of Nbhin1, a HR marker gene”
Claim · model-written summary
Silenced plants inoculated with R. solanacearum showed drastically increased expression of genes for salicylic acid and jasmonic acid signaling.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbPLC4-1- and NbPLC4-2-silencede1
- Interactor
- R. solanacearumRalstonia solanacearume1pathogen
- Outcome
- genes for salicylic acid (SA) and jasmonic acid (JA) signalinge1
- Direction
- drastically increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e1“Expressions of genes for salicylic acid (SA) and jasmonic acid (JA) signaling were drastically increased in NbPLC4-1- and NbPLC4-2-silenced plants by R. solanacearum inoculation”
Claim · model-written summary
NbPLC4-1 and NbPLC4-2 silencing triggered reactive oxygen species hyper-production.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbPLC4-1 and NbPLC4-2 silencinge1
- Gene
- NbPLC4-1 and NbPLC4-2e1
- Outcome
- reactive oxygen species (ROS) hyper-productione1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e1“In addition, NbPLC4-1 and NbPLC4-2 silencing triggered reactive oxygen species (ROS) hyper-production”
Claim · model-written summary
NbPLC4 orthologs are closely associated with JA, SA and ROS signaling.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbPLC4se1
- Outcome
- JA, SA, and ROS signalinge1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e1“These results suggest that NbPLC4s are closely associated with JA, SA, and ROS signaling and act as negative regulators of the HR in N. benthamiana”
Claim · model-written summary
NbPLC4s act as negative regulators of the hypersensitive response.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbPLC4se1
- Outcome
- the HRe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e1“These results suggest that NbPLC4s are closely associated with JA, SA, and ROS signaling and act as negative regulators of the HR in N. benthamiana”
The same questions in other papers
loss of function · physiology: oxidative · Nicotiana benthamianaPositive and negative results
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- negative
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- negative
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