Abstract
Peptide transport is important for plant tissues where rapid proteolysis occurs, especially during germination and senescence, to enhance redistribution of organic nitrogen (N). However, the biological role of peptide transporters is poorly investigated in rice. We characterized the function of the peptide transporter OsNPF8.1 of rice nitrate transporter 1/peptide transporter family (NPF). Ectopic expression of OsNPF8.1 in yeast revealed that OsNPF8.1 encoded a high-affinity di-/tri-peptide transporter, and the osnpf8.1 mutants had a lower uptake rate of the fluorescent-labelled dipeptide c in leaves of rice seedlings. Histochemical assays showed that OsNPF8.1 was highly expressed in mesophyll cells and vascular parenchyma cells, but not detected in root hairs and epidermises. Expression of OsNPF8.1 was induced by N deficiency, drought, NaCl and abscisic acid, and kept at a high level in senescing leaves. Under N deficiency conditions, compared with the wild type Zhonghua 11, the osnpf8.1 mutants grew slower at the seedling stage, and had lower grain yield and lower N content in the grains. In contrast, OsNPF8.1-over-expressing rice (OsNPF8.1-OE) grew faster at the seedling stage and had a higher grain yield. The osnpf8.1 seedlings were less tolerant to salt and drought stresses. These results suggested that stress-induced organic N transportation mediated by OsNPF8.1 might contribute to balance plant growth and tolerate to salt/drought stress and N-deficiency.
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Claims 12
Claim · model-written summary
OsNPF8.1 encoded a high-affinity di-/tri-peptide transporter in yeast.
- Taxon
- Rice
Oryza sativat0 - Gene
- OsNPF8.1e0
- Outcome
- high-affinity di-/tri-peptide transportere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsNPF8.1 encoded a high-affinity di-/tri-peptide transporter”
t0“Rice”
Claim · model-written summary
Osnpf8.1 mutant rice seedlings had lower leaf uptake of fluorescent-labelled dipeptide c.
- Taxon
- riceOryza sativae0
- Material
- osnpf8.1 mutantse0
- Organ / stage
- leavese0
- Treatment
- osnpf8.1 mutantse0
- Outcome
- uptake rate of the fluorescent-labelled dipeptide ce0
- Direction
- lowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the osnpf8.1 mutants had a lower uptake rate of the fluorescent-labelled dipeptide c in leaves of rice seedlings”
Claim · model-written summary
OsNPF8.1 was highly expressed in mesophyll and vascular parenchyma cells.
- Taxon
- RiceOryza sativat0
- Organ / stage
- mesophyll cells and vascular parenchyma cellse0
- Gene
- OsNPF8.1e0
- Outcome
- highly expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsNPF8.1 was highly expressed in mesophyll cells and vascular parenchyma cells”
t0“Rice”
Claim · model-written summary
OsNPF8.1 expression was undetected in root hairs and epidermises.
- Taxon
- RiceOryza sativat1
- Organ / stage
- root hairs and epidermisese0
- Gene
- OsNPF8.1t0
- Outcome
- not detectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not detected in root hairs and epidermises”
t0“Peptide Transporter OsNPF8.1”
t1“Rice”
e1“OsNPF8.1 was highly expressed in mesophyll cells and vascular parenchyma cells”
Claim · model-written summary
N deficiency, drought, NaCl and abscisic acid induced OsNPF8.1 expression.
- Taxon
- RiceOryza sativat0
- Treatment
- N deficiency, drought, NaCl and abscisic acide0
- Gene
- OsNPF8.1e0
- Outcome
- Expression of OsNPF8.1e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsNPF8.1 was induced by N deficiency, drought, NaCl and abscisic acid”
t0“Rice”
Claim · model-written summary
OsNPF8.1 expression remained high in senescing leaves.
- Taxon
- RiceOryza sativat1
- Organ / stage
- senescing leavese0
- Gene
- OsNPF8.1t0
- Outcome
- high levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“kept at a high level in senescing leaves”
t0“Peptide Transporter OsNPF8.1”
t1“Rice”
Claim · model-written summary
Under nitrogen deficiency, osnpf8.1 mutants grew slower at seedling stage than wild type Zhonghua 11.
- Taxon
- RiceOryza sativat0
- Material
- osnpf8.1 mutantse0
- Organ / stage
- seedling stagee0
- Treatment
- N deficiency conditionse0
- Outcome
- grewe0
- Direction
- slowere0
- Comparator
- wild type Zhonghua 11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under N deficiency conditions, compared with the wild type Zhonghua 11, the osnpf8.1 mutants grew slower at the seedling stage”
t0“Rice”
Claim · model-written summary
Under nitrogen deficiency, osnpf8.1 mutants had lower grain yield than wild type Zhonghua 11.
- Taxon
- RiceOryza sativat0
- Material
- osnpf8.1 mutantse0
- Treatment
- N deficiency conditionse0
- Outcome
- grain yielde0
- Direction
- lowere0
- Comparator
- wild type Zhonghua 11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under N deficiency conditions, compared with the wild type Zhonghua 11, the osnpf8.1 mutants grew slower at the seedling stage, and had lower grain yield and lower N content in the grains”
t0“Rice”
Claim · model-written summary
Under nitrogen deficiency, osnpf8.1 mutants had lower grain N content than wild type Zhonghua 11.
- Taxon
- RiceOryza sativat0
- Material
- osnpf8.1 mutantse0
- Treatment
- N deficiency conditionse0
- Outcome
- N content in the grainse0
- Direction
- lowere0
- Comparator
- wild type Zhonghua 11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under N deficiency conditions, compared with the wild type Zhonghua 11, the osnpf8.1 mutants grew slower at the seedling stage, and had lower grain yield and lower N content in the grains”
t0“Rice”
Claim · model-written summary
OsNPF8.1 overexpressing rice grew faster at seedling stage.
- Taxon
- riceOryza sativae0
- Material
- OsNPF8.1-OEe0
- Treatment
- OsNPF8.1-over-expressinge0
- Outcome
- grewe0
- Direction
- fastere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsNPF8.1-over-expressing rice (OsNPF8.1-OE) grew faster at the seedling stage”
Claim · model-written summary
OsNPF8.1 overexpressing rice had higher grain yield.
- Taxon
- riceOryza sativae0
- Material
- OsNPF8.1-OEe0
- Treatment
- OsNPF8.1-over-expressinge0
- Outcome
- grain yielde0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsNPF8.1-over-expressing rice (OsNPF8.1-OE) grew faster at the seedling stage and had a higher grain yield”
Claim · model-written summary
Osnpf8.1 seedlings were less tolerant to salt and drought stress.
- Taxon
- RiceOryza sativat0
- Material
- osnpf8.1 seedlingse0
- Treatment
- salt and drought stressese0
- Outcome
- tolerante0
- Direction
- lesse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The osnpf8.1 seedlings were less tolerant to salt and drought stresses”
t0“Rice”
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