Abstract
In the present investigation, optimization of the best concentration combinations of BAP (Benzyl Amino Purine), TDZ (Thidiazuron) and Silver Nitrate (AgNO3) for micropropagation of carnation (Dianthus caryophyllus L.) cv. Irene was performed. Genetic fidelity in the microplants of different generation was determined with the help of ISSR markers. Results of the present investigation showed that the MS gelled media supplemented with BAP (0.5 mg L–1) and AgNO3 (1.5 mg L–1) showed maximum shoot proliferation, good shoot length and reduced hyperhydricity significantly. Our study also indicates that hyperhydricity decreased by substantially by increasing the concentration of AgNO3 compared to control. High level of AgNO3 in MS medium did not helped to reduce hyperhydricity. Another chemical combination of TDZ (1.0 mg L–1) and AgNO3 (0.5 mg L–1) in MS gelled media gave the maximum shoot proliferation, shoot length with minimum hyperhydricity which showed normal growth and development compared to control and other treatments. This concentration combination proved effective to reduce hyperhydricity and to enhance shoot proliferation. Because TDZ with AgNO3 had pronounced effect to reduce hyperhydricity, therefore, this concentration combination was used to advance the progeny up to subculture (SC10) using donor mother microplants (SC1) selected from the best concentration response to know the genetic fidelity among the different generations. Microplants which were non hyperhydrified were used to assess the genetic fidelity using ISSR markers. Fourteen ISSR primers were used that gave good amplification and produced 616 bands with an average of 44 amplicon per primer from in vitro raised microplants including mother plant where the banding patterns exhibited uniform and homogeneous banding patterns. This study may help us to know applicability of ISSR markers to establish genetic fidelity among in vitro—raised microplants of carnation.
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Claims 11
Claim · model-written summary
BAP and AgNO3 media produced maximum carnation shoot proliferation.
- Taxon
- Carnation
Dianthus caryophyllust0 - Treatment
- BAP (0.5 mg L–1) and AgNO3 (1.5 mg L–1)e0
- Outcome
- shoot proliferatione0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS gelled media supplemented with BAP (0.5 mg L–1) and AgNO3 (1.5 mg L–1) showed maximum shoot proliferation, good shoot length and reduced hyperhydricity significantly”
t0“Carnation (Dianthus caryophyllus L.)”
Claim · model-written summary
BAP and AgNO3 media produced good carnation shoot length.
- Taxon
- CarnationDianthus caryophyllust0
- Treatment
- BAP (0.5 mg L–1) and AgNO3 (1.5 mg L–1)e0
- Outcome
- shoot lengthe0
- Direction
- goode0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS gelled media supplemented with BAP (0.5 mg L–1) and AgNO3 (1.5 mg L–1) showed maximum shoot proliferation, good shoot length and reduced hyperhydricity significantly”
t0“Carnation (Dianthus caryophyllus L.)”
Claim · model-written summary
BAP and AgNO3 media significantly reduced carnation hyperhydricity.
- Taxon
- CarnationDianthus caryophyllust0
- Treatment
- BAP (0.5 mg L–1) and AgNO3 (1.5 mg L–1)e0
- Outcome
- hyperhydricitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS gelled media supplemented with BAP (0.5 mg L–1) and AgNO3 (1.5 mg L–1) showed maximum shoot proliferation, good shoot length and reduced hyperhydricity significantly”
t0“Carnation (Dianthus caryophyllus L.)”
Claim · model-written summary
Increasing AgNO3 concentration substantially decreased hyperhydricity versus control.
- Taxon
- CarnationDianthus caryophyllust0
- Treatment
- increasing the concentration of AgNO3e0
- Outcome
- hyperhydricitye0
- Direction
- decreasede0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hyperhydricity decreased by substantially by increasing the concentration of AgNO3 compared to control”
t0“Carnation (Dianthus caryophyllus L.)”
Claim · model-written summary
High AgNO3 levels did not reduce hyperhydricity.
- Taxon
- CarnationDianthus caryophyllust0
- Treatment
- High level of AgNO3 in MS mediume0
- Outcome
- hyperhydricitye0
- Direction
- did not helped to reducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High level of AgNO3 in MS medium did not helped to reduce hyperhydricity”
t0“Carnation (Dianthus caryophyllus L.)”
Claim · model-written summary
TDZ and AgNO3 media maximized carnation shoot proliferation relative to control and other treatments.
- Taxon
- CarnationDianthus caryophyllust0
- Treatment
- TDZ (1.0 mg L–1) and AgNO3 (0.5 mg L–1)e0
- Outcome
- shoot proliferatione0
- Direction
- maximume0
- Comparator
- control and other treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TDZ (1.0 mg L–1) and AgNO3 (0.5 mg L–1) in MS gelled media gave the maximum shoot proliferation, shoot length with minimum hyperhydricity which showed normal growth and development compared to control and other treatments”
t0“Carnation (Dianthus caryophyllus L.)”
Claim · model-written summary
TDZ and AgNO3 media maximized carnation shoot length.
- Taxon
- CarnationDianthus caryophyllust0
- Treatment
- TDZ (1.0 mg L–1) and AgNO3 (0.5 mg L–1)e0
- Outcome
- shoot lengthe0
- Direction
- maximume0
- Comparator
- control and other treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TDZ (1.0 mg L–1) and AgNO3 (0.5 mg L–1) in MS gelled media gave the maximum shoot proliferation, shoot length with minimum hyperhydricity which showed normal growth and development compared to control and other treatments”
t0“Carnation (Dianthus caryophyllus L.)”
Claim · model-written summary
TDZ and AgNO3 media minimized carnation hyperhydricity.
- Taxon
- CarnationDianthus caryophyllust0
- Treatment
- TDZ (1.0 mg L–1) and AgNO3 (0.5 mg L–1)e0
- Outcome
- hyperhydricitye0
- Direction
- minimume0
- Comparator
- control and other treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TDZ (1.0 mg L–1) and AgNO3 (0.5 mg L–1) in MS gelled media gave the maximum shoot proliferation, shoot length with minimum hyperhydricity which showed normal growth and development compared to control and other treatments”
t0“Carnation (Dianthus caryophyllus L.)”
Claim · model-written summary
TDZ and AgNO3 media produced normal carnation growth and development.
- Taxon
- CarnationDianthus caryophyllust0
- Treatment
- TDZ (1.0 mg L–1) and AgNO3 (0.5 mg L–1)e0
- Outcome
- normal growth and developmente0
- Comparator
- control and other treatmentse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TDZ (1.0 mg L–1) and AgNO3 (0.5 mg L–1) in MS gelled media gave the maximum shoot proliferation, shoot length with minimum hyperhydricity which showed normal growth and development compared to control and other treatments”
t0“Carnation (Dianthus caryophyllus L.)”
Claim · model-written summary
ISSR primers produced 616 bands, averaging 44 amplicons per primer in microplants.
- Taxon
- CarnationDianthus caryophyllust0
- Outcome
- bandse0
- Value
- 616 bandse0
- Qualifier
- average of 44 amplicon per primere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fourteen ISSR primers were used that gave good amplification and produced 616 bands with an average of 44 amplicon per primer from in vitro raised microplants including mother plant”
t0“Carnation (Dianthus caryophyllus L.)”
Claim · model-written summary
ISSR banding patterns were uniform and homogeneous among microplants and mother plant.
- Taxon
- CarnationDianthus caryophyllust0
- Material
- microplantse1
- Outcome
- uniform and homogeneous banding patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“banding patterns exhibited uniform and homogeneous banding patterns”
e1“from in vitro raised microplants including mother plant”
t0“Carnation (Dianthus caryophyllus L.)”
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