Abstract
To determine whether Azospirillum brasilense mitigates water stress and lowers nitrogen fertilizer consumption in maize, physiology (pigments, nitrate reductase gas exchange, and water potential), macro/micronutrients, root shape, and biomass were studied. The experiment was carried out in a greenhouse and included seven treatments and four replications. Plants under water deficits were inoculated with Azospirillum strains (Az1 and Az2) under two nitrogen fertilization conditions (20 and 120 kg ha−1) and compared to control plants (with irrigation and fertilization of 120 kg ha−1 without inoculation). Inoculation with Az1 increased the pigment content under water stress, mainly in the 120 kg ha−1 nitrogen treatment. The maize root system showed the best response to inoculation with A. brasilense. The plants with inoculant Az1 at 120 kg ha−1 N responded similar to the control plants, which were under ideal growing conditions for length, surface area, root diameter and shoot and root dry mass. Inoculation with A. brasilense Az1 and 120 kg ha−1 nitrogen under water stress improved the physiology and root morphology of maize. However, the use of inoculation does not replace nitrogen fertilization in maize, and the possible reasons and consequences are discussed. Inoculation of plants with A. brasilense is an effective strategy that should be applied in maize production.
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Claims 5
Claim · model-written summary
Az1 inoculation increased pigment content under water stress.
- Treatment
- Inoculation with Az1e0
- Interactor
- Az1e0symbiont
- Outcome
- pigment contente0
- Direction
- increasede0
- Qualifier
- under water stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Inoculation with Az1 increased the pigment content under water stress, mainly in the 120 kg ha−1 nitrogen treatment.”
t0“maize”
Claim · model-written summary
Az1 with 120 kg ha−1 nitrogen gave root and dry mass responses similar to irrigated controls.
- Treatment
- inoculant Az1 at 120 kg ha−1 Ne0
- Interactor
- Az1e0symbiont
- Outcome
- length, surface area, root diameter and shoot and root dry masse0
- Direction
- similare0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The plants with inoculant Az1 at 120 kg ha−1 N responded similar to the control plants, which were under ideal growing conditions for length, surface area, root diameter and shoot and root dry mass.”
t0“maize”
Claim · model-written summary
Az1 inoculation with 120 kg ha−1 nitrogen improved maize physiology and root morphology under water stress.
- Treatment
- Inoculation with A. brasilense Az1 and 120 kg ha−1 nitrogene0
- Interactor
- A. brasilense Az1
Azospirillum brasilensee0symbiont - Outcome
- physiology and root morphologye0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.


Verbatim
e0“Inoculation with A. brasilense Az1 and 120 kg ha−1 nitrogen under water stress improved the physiology and root morphology of maize.”
Claim · model-written summary
Inoculation did not replace nitrogen fertilization in maize.
- Outcome
- does not replace nitrogen fertilizatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the use of inoculation does not replace nitrogen fertilization in maize”
Claim · model-written summary
The authors recommend A. brasilense inoculation in maize production.
- Interactor
- A. brasilense
Azospirillum brasilensee0symbiont - Outcome
- should be applied in maize productione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.


Verbatim
e0“Inoculation of plants with A. brasilense is an effective strategy that should be applied in maize production.”
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