Abstract
Abstract Background Potato ( Solanum tuberosum L.) is one of the most economically important annual vegetable crops. Microtubers can be produced, stored around year and directly transported to market without transferring to fresh media and without acclimatization. Thus reducing the field cycle to obtain sufficient number of seed potatoes and a high level of healthy materials. The aim of this study was to investigate effects of different combinations and concentrations of growth regulators i.e., kinetin (2.5 mg/l) and coumarin (20, 40 and 60 mg/l) alone or in addition to varied concentrations of sucrose (30, 60 and 90 g/l) on in vitro tuberization of potato ( Solanum tuberosum ) cv. Diamount. Results The plantlets were propagated using single-node cuttings cultured on Murashige and Skoog medium containing 0.04 mg/l kinetin and 1.0 mg/l IAA. Shoots (6–7 nodes) from the previous step were cultured into medium supplemented with a factorial combination of sucrose concentrations (30, 60 and 90 g/l), coumarin concentrations (0, 20, 40, and 60 mg/l) and two concentrations of kinetin (0 and 2.5 mg/l). Our results showed that kinetin – induced medium have slightly effect to improve tuberization in vitro . But when kinetin was combined with sucrose, this effect was better when raised sucrose concentration from 30 or 60 to 90 g/l. The highest percentage of tuberization after 8 weeks, highest number of microtuber were obtained with high concentration of sucrose (90 g/l) together with dark condition, at 18–20 °C. Also, the highest concentration of sucrose significantly increased the fresh weight of microtuber. In case of coumarin, results revealed that it has important effect on tuber initiation especially with concentrations 20 mg/l and 40 mg/l; otherwise, the initiation period was minimized after adding kinetin. So, the plantlets must be continuously incubated in coumarin –induce medium plus kinetin for 8 weeks to affect the tuberization response. Also, all the other characters of tuberization process on coumarin-inducing medium could be improved by increasing sucrose concentration to 9 g/l. Conclusions Generally, results pointed out that the treatment which consists of kinetin (2.5 mg/l) plus 90 g/l sucrose and 20 mg/l coumarin has the best characteristics of in vitro micro-tuberization.
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Claims 10
Claim · model-written summary
Kinetin slightly improved potato tuberization in vitro.
- Taxon
- potato
Solanum tuberosumt0 - Treatment
- kinetine0
- Outcome
- tuberization in vitroe0
- Direction
- improvee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“kinetin – induced medium have slightly effect to improve tuberization in vitro”
t0“potato”
Claim · model-written summary
Combining kinetin with sucrose improved tuberization more at 90 than at 30 or 60 g/l sucrose.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- kinetin was combined with sucrosee0
- Outcome
- this effect was bettere0
- Direction
- bettere0
- Value
- 90 g/le0
- Comparator
- 30 or 60e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when kinetin was combined with sucrose, this effect was better when raised sucrose concentration from 30 or 60 to 90 g/l”
t0“potato”
Claim · model-written summary
At 90 g/l sucrose in dark conditions at 18–20 °C, the tuberization percentage after eight weeks was highest.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- high concentration of sucrose (90 g/l) together with dark condition, at 18–20 °Ce0
- Outcome
- percentage of tuberizatione0
- Direction
- higheste0
- Qualifier
- after 8 weekse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest percentage of tuberization after 8 weeks, highest number of microtuber were obtained with high concentration of sucrose (90 g/l) together with dark condition, at 18–20 °C”
t0“potato”
Claim · model-written summary
At 90 g/l sucrose in dark conditions at 18–20 °C, the microtuber number was highest.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- high concentration of sucrose (90 g/l) together with dark condition, at 18–20 °Ce0
- Outcome
- number of microtubere0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“highest number of microtuber were obtained with high concentration of sucrose (90 g/l) together with dark condition, at 18–20 °C”
t0“potato”
Claim · model-written summary
The highest sucrose concentration significantly increased microtuber fresh weight.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- highest concentration of sucrosee0
- Outcome
- fresh weight of microtubere0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest concentration of sucrose significantly increased the fresh weight of microtuber”
t0“potato”
Claim · model-written summary
Coumarin at 20 and 40 mg/l affected tuber initiation.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- coumarine0
- Outcome
- tuber initiatione0
- Qualifier
- 20 mg/l and 40 mg/le0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“coumarin, results revealed that it has important effect on tuber initiation especially with concentrations 20 mg/l and 40 mg/l”
t0“potato”
Claim · model-written summary
Adding kinetin shortened the tuber initiation period.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- adding kinetine0
- Outcome
- initiation periode0
- Direction
- minimizede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the initiation period was minimized after adding kinetin”
t0“potato”
Claim · model-written summary
Increasing sucrose to 9 g/l improved other tuberization characteristics on coumarin medium, as stated.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- increasing sucrose concentration to 9 g/le0
- Outcome
- all the other characters of tuberization processe0
- Direction
- improvede0
- Qualifier
- coumarin-inducing mediume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all the other characters of tuberization process on coumarin-inducing medium could be improved by increasing sucrose concentration to 9 g/l”
t0“potato”
Claim · model-written summary
The authors recommend eight weeks of incubation in medium with coumarin and kinetin for tuberization.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- continuously incubated in coumarin –induce medium plus kinetin for 8 weekse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the plantlets must be continuously incubated in coumarin –induce medium plus kinetin for 8 weeks to affect the tuberization response”
t0“potato”
Claim · model-written summary
The authors judged kinetin at 2.5 mg/l with 90 g/l sucrose and 20 mg/l coumarin the best microtuberization treatment.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- kinetin (2.5 mg/l) plus 90 g/l sucrose and 20 mg/l coumarine0
- Outcome
- best characteristics of in vitro micro-tuberizatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“kinetin (2.5 mg/l) plus 90 g/l sucrose and 20 mg/l coumarin has the best characteristics of in vitro micro-tuberization”
t0“potato”
The same questions in other papers
sucrose · growth · Solanum tuberosum (potato)Positive and negative results
12 papers in the same direction as this paper · 3 in the opposite direction · 1 reporting no difference from an alternative
- negative
- no effect
- negative
13 papers more in this group
See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.