Paper · 2013 · Sinai Journal of Applied Sciences/Sinai Journal of Applied Sciences

Genetic Evaluation and Molecular Markers for Heat Tolerance in Tomato (Lycopersicon esculentum Mill.)

M Kamel; S Soliman; A Mandour; et al.

15
claims
30
verbatim quotes
8
citations
4
research areas
✓
Open access

Abstract

Genetic evaluation was performed on twenty three genotypes of tomato (Lycopersicon esculentum Mill.) under high temperature at summer season to determine the variation between them for heat tolerance. Heat tolerance related criteria, i.e., pollen viability, fruit setting, osmotic pressure and fruit yield per plant. LSSS1, Homestead 24, Black Russian Plum, Super Marmand and Money Maker possess more tolerance of heat. In contrast, Super Stain B, Castle Rock, Cherokee Purple, Moskvich and Nicholevna Pink were more susceptible of heat. The pollen grain viability and fruit setting criteria consider as suitable morphological markers for heat tolerance than other heat tolerant related criteria as osmotic pressure. Heritability was high and moderately whereas, the genetic improvement of new strains could be done. From previous evaluation, Lsss1 as tolerant line and Super Strain B as sensitive cultivar of heat tolerance was crossed for study of molecular markers related to heat tolerance by using bulk segregate analysis (BSA). Crossing was carried out between these two genotypes to obtain the F1seeds which were left for selfing to obtain the F2 seeds. The two selected genotypes, their F1 and F2 plants were evaluated for their response to heat stress by recording some heat stress related traits. Bulk of the two extremely F2 plants (most tolerant and most sensitive F2 groups), the two contrasting parents and their F1, were used to develop some molecular genetic markers associated with heat tolerance in tomato by using ten RAPD and six ISSR primers. two RAPD markers (with molecular sizes of 100 bp for primers A16 and 500 bp for primer Z13) and one ISSR marker (with molecular size of 650 bp) were considered as reliable markers for heat tolerance as well as susceptible genotypes possessed eight RAPD markers (with molecular sizes 500 and 1500 bp for primer C02, 1750 and750 bp for primer C03, 2400 bp for primer C05, 550 bp for primer C08, 400 bp for primer C14 and 650 bp for primer C15). [Journal of American Science 2010; 6(12):364-374]. (ISSN: 1545-1003).

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Claims 15

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

LSSS1 was more tolerant of heat in tomato.

Material
LSSS1e2
Outcome
tolerance of heate2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Genetic evaluation was performed on twenty three genotypes of tomato (Lycopersicon esculentum Mill.) under high temperature at summer season”

✓ located verbatim in the source · abstract, characters 0–140 · show in text ↑
e2

“LSSS1, Homestead 24, Black Russian Plum, Super Marmand and Money Maker possess more tolerance of heat”

✓ located verbatim in the source · abstract, characters 317–418 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Homestead 24 was more tolerant of heat in tomato.

Material
Homestead 24e2
Outcome
tolerance of heate2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Genetic evaluation was performed on twenty three genotypes of tomato (Lycopersicon esculentum Mill.) under high temperature at summer season”

✓ located verbatim in the source · abstract, characters 0–140 · show in text ↑
e2

“LSSS1, Homestead 24, Black Russian Plum, Super Marmand and Money Maker possess more tolerance of heat”

✓ located verbatim in the source · abstract, characters 317–418 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Black Russian Plum was more tolerant of heat in tomato.

Material
Black Russian Plume2
Outcome
tolerance of heate2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Genetic evaluation was performed on twenty three genotypes of tomato (Lycopersicon esculentum Mill.) under high temperature at summer season”

✓ located verbatim in the source · abstract, characters 0–140 · show in text ↑
e2

“LSSS1, Homestead 24, Black Russian Plum, Super Marmand and Money Maker possess more tolerance of heat”

✓ located verbatim in the source · abstract, characters 317–418 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Super Marmand was more tolerant of heat in tomato.

Material
Super Marmande2
Outcome
tolerance of heate2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Genetic evaluation was performed on twenty three genotypes of tomato (Lycopersicon esculentum Mill.) under high temperature at summer season”

✓ located verbatim in the source · abstract, characters 0–140 · show in text ↑
e2

“LSSS1, Homestead 24, Black Russian Plum, Super Marmand and Money Maker possess more tolerance of heat”

✓ located verbatim in the source · abstract, characters 317–418 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Money Maker was more tolerant of heat in tomato.

Material
Money Makere2
Outcome
tolerance of heate2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Genetic evaluation was performed on twenty three genotypes of tomato (Lycopersicon esculentum Mill.) under high temperature at summer season”

✓ located verbatim in the source · abstract, characters 0–140 · show in text ↑
e2

“LSSS1, Homestead 24, Black Russian Plum, Super Marmand and Money Maker possess more tolerance of heat”

✓ located verbatim in the source · abstract, characters 317–418 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Super Stain B was more susceptible to heat in tomato.

Material
Super Stain Be2
Outcome
susceptible of heate2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Genetic evaluation was performed on twenty three genotypes of tomato (Lycopersicon esculentum Mill.) under high temperature at summer season”

✓ located verbatim in the source · abstract, characters 0–140 · show in text ↑
e2

“Super Stain B, Castle Rock, Cherokee Purple, Moskvich and Nicholevna Pink were more susceptible of heat”

✓ located verbatim in the source · abstract, characters 433–536 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Castle Rock was more susceptible to heat in tomato.

Material
Castle Rocke2
Outcome
susceptible of heate2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Genetic evaluation was performed on twenty three genotypes of tomato (Lycopersicon esculentum Mill.) under high temperature at summer season”

✓ located verbatim in the source · abstract, characters 0–140 · show in text ↑
e2

“Super Stain B, Castle Rock, Cherokee Purple, Moskvich and Nicholevna Pink were more susceptible of heat”

✓ located verbatim in the source · abstract, characters 433–536 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Cherokee Purple was more susceptible to heat in tomato.

Material
Cherokee Purplee2
Outcome
susceptible of heate2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Genetic evaluation was performed on twenty three genotypes of tomato (Lycopersicon esculentum Mill.) under high temperature at summer season”

✓ located verbatim in the source · abstract, characters 0–140 · show in text ↑
e2

“Super Stain B, Castle Rock, Cherokee Purple, Moskvich and Nicholevna Pink were more susceptible of heat”

✓ located verbatim in the source · abstract, characters 433–536 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Moskvich was more susceptible to heat in tomato.

Material
Moskviche2
Outcome
susceptible of heate2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Genetic evaluation was performed on twenty three genotypes of tomato (Lycopersicon esculentum Mill.) under high temperature at summer season”

✓ located verbatim in the source · abstract, characters 0–140 · show in text ↑
e2

“Super Stain B, Castle Rock, Cherokee Purple, Moskvich and Nicholevna Pink were more susceptible of heat”

✓ located verbatim in the source · abstract, characters 433–536 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Nicholevna Pink was more susceptible to heat in tomato.

Material
Nicholevna Pinke2
Outcome
susceptible of heate2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Genetic evaluation was performed on twenty three genotypes of tomato (Lycopersicon esculentum Mill.) under high temperature at summer season”

✓ located verbatim in the source · abstract, characters 0–140 · show in text ↑
e2

“Super Stain B, Castle Rock, Cherokee Purple, Moskvich and Nicholevna Pink were more susceptible of heat”

✓ located verbatim in the source · abstract, characters 433–536 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Pollen grain viability and fruit setting are suitable morphological markers for heat tolerance in tomato, more than osmotic pressure.

Outcome
suitable morphological markers for heat tolerancee2
Qualifier
pollen grain viability and fruit setting criteriae2
Comparator
osmotic pressuree2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Genetic evaluation was performed on twenty three genotypes of tomato (Lycopersicon esculentum Mill.) under high temperature at summer season”

✓ located verbatim in the source · abstract, characters 0–140 · show in text ↑
e2

“The pollen grain viability and fruit setting criteria consider as suitable morphological markers for heat tolerance than other heat tolerant related criteria as osmotic pressure”

✓ located verbatim in the source · abstract, characters 538–715 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Heritability for heat tolerance traits in tomato was high and moderate.

Outcome
Heritabilitye2
Qualifier
high and moderatelye2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Genetic evaluation was performed on twenty three genotypes of tomato (Lycopersicon esculentum Mill.) under high temperature at summer season”

✓ located verbatim in the source · abstract, characters 0–140 · show in text ↑
e2

“Heritability was high and moderately whereas, the genetic improvement of new strains could be done”

✓ located verbatim in the source · abstract, characters 717–815 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Two RAPD markers were considered reliable markers for heat tolerance in tomato.

Outcome
reliable markers for heat tolerancee2
Value
100 bpe2
Qualifier
two RAPD markers (with molecular sizes of 100 bp for primers A16 and 500 bp for primer Z13)e2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Genetic evaluation was performed on twenty three genotypes of tomato (Lycopersicon esculentum Mill.) under high temperature at summer season”

✓ located verbatim in the source · abstract, characters 0–140 · show in text ↑
e2

“two RAPD markers (with molecular sizes of 100 bp for primers A16 and 500 bp for primer Z13) and one ISSR marker (with molecular size of 650 bp) were considered as reliable markers for heat tolerance”

✓ located verbatim in the source · abstract, characters 1564–1762 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

One ISSR marker was considered a reliable marker for heat tolerance in tomato.

Outcome
reliable markers for heat tolerancee2
Value
650 bpe2
Qualifier
one ISSR marker (with molecular size of 650 bp)e2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Genetic evaluation was performed on twenty three genotypes of tomato (Lycopersicon esculentum Mill.) under high temperature at summer season”

✓ located verbatim in the source · abstract, characters 0–140 · show in text ↑
e2

“two RAPD markers (with molecular sizes of 100 bp for primers A16 and 500 bp for primer Z13) and one ISSR marker (with molecular size of 650 bp) were considered as reliable markers for heat tolerance”

✓ located verbatim in the source · abstract, characters 1564–1762 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Susceptible tomato genotypes possessed eight RAPD markers.

Material
susceptible genotypese2
Outcome
RAPD markerse2
Value
eighte2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Genetic evaluation was performed on twenty three genotypes of tomato (Lycopersicon esculentum Mill.) under high temperature at summer season”

✓ located verbatim in the source · abstract, characters 0–140 · show in text ↑
e2

“susceptible genotypes possessed eight RAPD markers”

✓ located verbatim in the source · abstract, characters 1774–1824 · show in text ↑