Abstract
The lack of healthy planting materials is one of the major constraints of sweet potato cultivation in Benin. One of the solutions to this problem is the use of vegetal biotechnology through micropropagation. At the end of the production of vitroplants, a crucial phase consists in acclimatizing them before their transfer to the real environment. The type of substrate used plays an important role in the success of acclimatization. This study aims at evaluating the effect of the type of substrates on the survival and growth of sweet potato vitroplants in acclimatization of the two accessions (Mèché and Bombo vôvô). For this, three substrates were assessed. These are Potting soil alone, Potting soil + Sawdust, and potting soil + Charcoal powder in proportion 2:1. These substrates were first sterilized separately in an oven at 150°C for 2 hours. The experiment was conducted in the greenhouse of Central Laboratory of Vegetal Biotechnology and Plant Improvement of the Faculty of Sciences and Techniques of the University of Abomey-Calavi following a model of random complete blocks in split plot design. The results show that the type of substrate influences the success of acclimatization in terms of survival rate, the gain of size and the number of neoformed leaves (p= 0.0033; 0.0019 and 0.0001). The Potting soil + Charcoal powder substrate influenced these parameters better than the other two with 95%, 3.13 cm and 3.165 respectively for survival rate, size gain and the number of neoformed leaves. It was followed by the Potting soil + Sawdust substrate. Acclimatized plants have been successfully introduced into the field, proving that this technique is fruitful in the increase of the production of healthy cuttings and make them available to farmers. The protocol used in this study can be applied in the acclimatization of sweet potato vitroplants.
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Claims 4
Claim · model-written summary
Potting soil with charcoal powder gave a better survival rate (95%) than the other two substrates in sweet potato vitroplants.
- Taxon
- sweet potato
_Flower.jpg?width=60)
Ipomoea batatast0 - Material
- Mèché and Bombo vôvôe3
- Treatment
- Potting soil + Charcoal powder substratee2
- Outcome
- survival ratee2
- Direction
- bettere2
- Value
- 95%e2
- Qualifier
- p= 0.0033e1
- Comparator
- the other twoe2
- Setting
- greenhousee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Flower.jpg?width=500)
Verbatim
t0“Sweet Potato Vitroplants”
e1“The results show that the type of substrate influences the success of acclimatization in terms of survival rate, the gain of size and the number of neoformed leaves (p= 0.0033; 0.0019 and 0.0001)”
e2“The Potting soil + Charcoal powder substrate influenced these parameters better than the other two with 95%, 3.13 cm and 3.165 respectively for survival rate, size gain and the number of neoformed leaves”
e3“the two accessions (Mèché and Bombo vôvô)”
e4“The experiment was conducted in the greenhouse of Central Laboratory of Vegetal Biotechnology”
Claim · model-written summary
Potting soil with charcoal powder gave a better size gain (3.13 cm) than the other two substrates in sweet potato vitroplants.
- Taxon
- sweet potatoIpomoea batatast0
- Material
- Mèché and Bombo vôvôe3
- Treatment
- Potting soil + Charcoal powder substratee2
- Outcome
- size gaine2
- Direction
- bettere2
- Value
- 3.13 cme2
- Comparator
- the other twoe2
- Setting
- greenhousee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potato Vitroplants”
e1“The results show that the type of substrate influences the success of acclimatization in terms of survival rate, the gain of size and the number of neoformed leaves (p= 0.0033; 0.0019 and 0.0001)”
e2“The Potting soil + Charcoal powder substrate influenced these parameters better than the other two with 95%, 3.13 cm and 3.165 respectively for survival rate, size gain and the number of neoformed leaves”
e3“the two accessions (Mèché and Bombo vôvô)”
e4“The experiment was conducted in the greenhouse of Central Laboratory of Vegetal Biotechnology”
Claim · model-written summary
Potting soil with charcoal powder gave a better number of neoformed leaves (3.165) than the other two substrates in sweet potato vitroplants.
- Taxon
- sweet potatoIpomoea batatast0
- Material
- Mèché and Bombo vôvôe3
- Treatment
- Potting soil + Charcoal powder substratee2
- Outcome
- the number of neoformed leavese2
- Direction
- bettere2
- Value
- 3.165e2
- Comparator
- the other twoe2
- Setting
- greenhousee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potato Vitroplants”
e1“The results show that the type of substrate influences the success of acclimatization in terms of survival rate, the gain of size and the number of neoformed leaves (p= 0.0033; 0.0019 and 0.0001)”
e2“The Potting soil + Charcoal powder substrate influenced these parameters better than the other two with 95%, 3.13 cm and 3.165 respectively for survival rate, size gain and the number of neoformed leaves”
e3“the two accessions (Mèché and Bombo vôvô)”
e4“The experiment was conducted in the greenhouse of Central Laboratory of Vegetal Biotechnology”
Claim · model-written summary
Acclimatized sweet potato plants were successfully introduced into the field.
- Taxon
- sweet potatoIpomoea batatast0
- Outcome
- introduced into the fielde5
- Setting
- fielde5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potato Vitroplants”
e5“Acclimatized plants have been successfully introduced into the field”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.